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Early detection and identification of pathogenic bacteria such as Escherichia coli (E. coli) is an essential task for public health. The conventional culture-based methods for bacterial colony detection usually take >24 hours to get the…

Developing rapid methods for pathogen detection and growth monitoring at low cell and analyte concentrations is an important goal, which numerous technologies are working towards solving. Rapid biosensors have already made a dramatic impact…

A hallmark of bacterial populations cultured in vitro is their homogeneity of growth, where the majority of cells display identical growth rate, cell size and content. Recent insights, however, have revealed that even cells growing in…

Quantitative Methods · Quantitative Biology 2022-06-07 Santosh Pandey , Yunsoo Park , Ankita Ankita , Gregory J. Phillips

A Rapid and cost-effective method for detecting bacterial cells on surfaces is critical to protect public health from various aspects, including food safety, clinical hygiene, and pharmacy quality. Herein, we first established an optical…

Quantitative Methods · Quantitative Biology 2024-03-13 Yuzhen Zhang , Zili Gao , Lili He

The Annotated Germs for Automated Recognition (AGAR) dataset is an image database of microbial colonies cultured on agar plates. It contains 18000 photos of five different microorganisms as single or mixed cultures, taken under diverse…

Computer Vision and Pattern Recognition · Computer Science 2021-08-04 Sylwia Majchrowska , Jarosław Pawłowski , Grzegorz Guła , Tomasz Bonus , Agata Hanas , Adam Loch , Agnieszka Pawlak , Justyna Roszkowiak , Tomasz Golan , Zuzanna Drulis-Kawa

Antimicrobial susceptibility testing (AST) is a standard clinical procedure used to quantify antimicrobial resistance (AMR). Currently, the gold standard method requires incubation for 18-24 h and subsequent inspection for growth by a…

Automated recognition and classification of bacteria species from microscopic images have significant importance in clinical microbiology. Bacteria classification is usually carried out manually by biologists using different shapes and…

Quantitative Methods · Quantitative Biology 2019-12-19 Muhammed Talo

Sepsis is a life-threatening condition which requires rapid diagnosis and treatment. Traditional microbiological methods are time-consuming and expensive. In response to these challenges, deep learning algorithms were developed to identify…

A rapid, cost-effective and easy method that allows on-site determination of the concentration of live and dead bacterial cells using a fibre-based spectroscopic device (the optrode system) is proposed and demonstrated. Identification of…

Quantitative Methods · Quantitative Biology 2018-09-12 Fang Ou , Cushla McGoverin , Simon Swift , Frédérique Vanholsbeeck

Raman spectroscopy is a promising tool for microbial identification, yet its implementation in microbiology and clinical workflow is still restricted due to the accompanying additional preparation required to focus on microbial signals.…

Quantitative Methods · Quantitative Biology 2026-03-19 Jeong Hee Kim , Jia Dong , Marissa Morales , Loza Tadesse

A disconcerting ramification of water pollution caused by burgeoning populations, rapid industrialization and modernization of agriculture, has been the exponential increase in the incidence of algal growth across the globe. Harmful algal…

Computer Vision and Pattern Recognition · Computer Science 2018-11-28 Arabinda Samantaray , Baijian Yang , J. Eric Dietz , Byung-Cheol Min

We developed a digital inline holography (DIH) system integrated with deep learning algorithms for real-time detection of particulate matter (PM) and bacterial contamination in peritoneal dialysis (PD) fluids. The system comprises a…

Optics · Physics 2024-11-05 Nicholas Bravo-Frank , Nicolas Mesyngier , Lei Feng , Jiarong Hong

This work presents twelve fine-tuned deep learning architectures to solve the bacterial classification problem over the Digital Image of Bacterial Species Dataset. The base architectures were mainly published as mobile or efficient…

Computer Vision and Pattern Recognition · Computer Science 2021-06-14 R. Gallardo García , S. Jarquín Rodríguez , B. Beltrán Martínez , C. Hernández Gracidas , R. Martínez Torres

Bacterial infectious diseases are a major threat to human health. Timely and sensitive pathogenic bacteria detection is crucial in identifying the bacterial contaminations and preventing the spread of infectious diseases. Due to limitations…

We report here on the ability of elastic light scattering in discriminating Gram+, Gram-and yeasts at an early stage of growth (6h). Our technique is non-invasive, low cost and does require neither skilled operators nor reagents. Therefore…

Traditional microbial detection methods often rely on the overall property of microbial cultures and cannot resolve individual growth event at high spatiotemporal resolution. As a result, they require bacteria to grow to confluence and then…

We introduce a new image cytometer design for detection of very small particulate and demonstrate its capability in water analysis. The device is a compact microscope composed of off the shelf components, such as a light emitting diode…

Optics · Physics 2016-02-17 Juan Miguel Pérez , Marc Jofre , Pedro Martínez , Valerio Pruneri

Aim: To evaluate a flow cytometry protocol that uses reference beads for the enumeration of live and dead bacteria present in a mixture. Methods and Results: Mixtures of live and dead Escherichia coli with live:dead concentration ratios…

Quantitative Methods · Quantitative Biology 2019-01-24 Fang Ou , Cushla McGoverin , Simon Swift , Frederique Vanholsbeeck

Deep learning has dramatically improved the performance in many application areas such as image classification, object detection, speech recognition, drug discovery and etc since 2012. Where deep learning algorithms promise to discover the…

Image and Video Processing · Electrical Eng. & Systems 2020-03-25 X. F. Xu , S. Talbot , T. Selvaraja

Counting microbial colonies is a fundamental task in microbiology and has many applications in numerous industry branches. Despite this, current studies towards automatic microbial counting using artificial intelligence are hardly…

Computer Vision and Pattern Recognition · Computer Science 2022-09-07 Sylwia Majchrowska , Jarosław Pawłowski , Natalia Czerep , Aleksander Górecki , Jakub Kuciński , Tomasz Golan
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