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Single Particle Tracking (SPT) can aid in understanding complex spatio-temporal processes. However, quantifying diffusivity and forces from individual live cell trajectories is complicated by inter- & intra-trajectory kinetic heterogeneity,…

Quantitative Methods · Quantitative Biology 2016-05-19 Christopher P. Calderon

Positron Emission Particle Tracking (PEPT) is an imaging method that tracks individual radioactive particles. PEPT relies on the detection of back-to-back photon pairs emitted by positron annihilation. It requires an algorithm to locate the…

Instrumentation and Detectors · Physics 2021-08-25 Sam Manger , Antoine Renaud , Jacques Vanneste

Time course measurement of single molecules on a cell surface provides detailed information on the dynamics of the molecules, which is otherwise inaccessible. To extract the quantitative information, single particle tracking (SPT) is…

Quantitative Methods · Quantitative Biology 2016-01-06 Shunsuke Teraguchi , Yutaro Kumagai

Diffusion coefficient measurements are important for many biological and material investigations, such as particle dynamics, kinetics, and size determinations. Amongst current measurement methods, single particle tracking (SPT) offers the…

Biomolecules · Quantitative Biology 2011-07-12 Shannon Kian Zareh , Michael C. DeSantis , Jonathan Kessler , Je-Luen Li , Y. M. Wang

In Real-Time, Feedback-Driven Single Particle Tracking methods, measurements of the emission intensity from a labeled, nanometer-scale particle are used in a feedback loop to track the motion of the particle as it moves inside its native…

Optics · Physics 2025-12-03 Md Faysal Hossain , Sean B. Andersson

Real-time feedback-driven single-particle tracking (RT-FD-SPT) is a class of techniques in the field of single-particle tracking that uses feedback control to keep a particle of interest in a detection volume. These methods provide high…

Chemical Physics · Physics 2022-09-29 Bertus van Heerden , Nicholas A. Vickers , Tjaart P. J. Krüger , Sean B. Andersson

Working from a Poisson-Gaussian noise model, a multi-sample extension of the Photon Counting Histogram Expectation Maximization (PCH-EM) algorithm is derived as a general-purpose alternative to the Photon Transfer (PT) method. This…

Instrumentation and Detectors · Physics 2024-03-08 Aaron J. Hendrickson , David P. Haefner , Stanley H. Chan , Nicholas R. Shade , Eric R. Fossum

Data assimilation methods aim at estimating the state of a system by combining observations with a physical model. When sequential data assimilation is considered, the joint distribution of the latent state and the observations is described…

Methodology · Statistics 2018-04-23 Thi Tuyet Trang Chau , Pierre Ailliot , Valérie Monbet , Pierre Tandeo

Single-particle tracking (SPT) has become a popular tool to study the intracellular transport of molecules in living cells. Inferring the character of their dynamics is important, because it determines the organization and functions of the…

Quantitative Methods · Quantitative Biology 2020-09-09 Joanna Janczura , Patrycja Kowalek , Hanna Loch-Olszewska , Janusz Szwabiński , Aleksander Weron

Single-particle tracking (SPT) grants unprecedented insight into cellular function at the molecular scale [1]. Throughout the cell, the movement of single-molecules is generally heterogeneous and complex. Hence, there is an imperative to…

Quantitative Methods · Quantitative Biology 2015-06-24 Mohamed El Beheiry , Maxime Dahan , Jean-Baptiste Masson

Experimental methods based on single particle tracking (SPT) are being increasingly employed in the physical and biological sciences, where nanoscale objects are visualized with high temporal and spatial resolution. SPT can probe…

Statistical Mechanics · Physics 2015-06-12 Denis Boyer , David S. Dean , Carlos Mejía-Monasterio , Gleb Oshanin

We consider approximate maximum likelihood parameter estimation in nonlinear state-space models. We discuss both direct optimization of the likelihood and expectation--maximization (EM). For EM, we also give closed-form expressions for the…

Methodology · Statistics 2015-11-03 Juho Kokkala , Arno Solin , Simo Särkkä

We present a novel filtering algorithm that employs Bayesian transfer learning to address the challenges posed by mismatched intensity of the noise in a pair of sensors, each of which tracks an object using a nonlinear dynamic system model.…

Systems and Control · Electrical Eng. & Systems 2026-05-19 Omar Alotaibi , Brian L. Mark , Mohammad Reza Fasihi

We continue studies of the uncertainty quantification problem in emission tomographies such as PET or SPECT when additional multimodal data (e.g., anatomical MRI images) are available. To solve the aforementioned problem we adapt the…

Machine Learning · Statistics 2021-12-03 Fedor Goncharov , Éric Barat , Thomas Dautremer

Single-molecule tracking (SMT) methods are under considerable expansion in many fields of cell biology, as the dynamics of cellular components in biological mechanisms becomes increasingly relevant. Despite the development of SMT…

Quantitative Methods · Quantitative Biology 2015-06-08 Sylvain Tollis

The detection of suspended single particles (SSPs) in water is a crucial element for monitoring water ecosystems. Although there are a variety of sensory methods, it is still an unrealized goal to combine multi-wavelength polarized light…

Optics · Physics 2024-10-31 Tong Liu

In single-photon light detection and ranging (SP-LiDAR) systems, the histogram distortion due to hardware dead time fundamentally limits the precision of depth estimation. To compensate for the dead time effects, the photon registration…

Signal Processing · Electrical Eng. & Systems 2024-07-04 Weijian Zhang , Hashan K. Weerasooriya , Prateek Chennuri , Stanley H. Chan

The moment method is an image analysis technique for sub-pixel estimation of particle positions. The total error in the calculated particle position includes effects of pixel locking and random noise in each pixel. Pixel locking, also known…

Plasma Physics · Physics 2011-05-23 Yan Feng , J. Goree , Bin Liu

Heterogeneous diffusion processes occur in many different fields such as transport in living cells or diffusion in porous media. A characterization of the transport parameters of such processes can be achieved by ensemble-based methods,…

Data Analysis, Statistics and Probability · Physics 2011-10-17 Michael Bauer , Rustem Valiullin , Günter Radons , Jörg Kärger

Single-molecule localization microscopy allows practitioners to locate and track labeled molecules in biological systems. When extracting diffusion coefficients from the resulting trajectories, it is common practice to perform a linear fit…

Biological Physics · Physics 2024-06-19 Jakob Tómas Bullerjahn , Gerhard Hummer
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