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Interferometric scattering microscopy was widely applied in nanoscopic detection and tracking due to its high sensitivity and label-free manner. The sensitivity is limited by contrast. Oblique illumination provided high contrast with lower…

Optics · Physics 2025-11-11 Xiang Zhang , Wenfu Lin , Yatao Yang , Yifan Wang

We present an entanglement-based quantitative phase gradient microscopy technique that employs principles from quantum ghost imaging and ghost diffraction. In this method, a transparent sample is illuminated by both photons of an entangled…

Quantum Physics · Physics 2026-03-09 Yingwen Zhang , Paul-Antoine Moreau , Duncan England , Ebrahim Karimi , Benjamin Sussman

Quantitative phase imaging (QPI) is a label-free computational imaging technique that provides optical path length information of specimens. In modern implementations, the quantitative phase image of an object is reconstructed digitally…

Optics · Physics 2022-05-23 Deniz Mengu , Aydogan Ozcan

In this paper, we propose a new model to segment cells in phase contrast microscopy images. Cell images collected from the similar scenario share a similar background. Inspired by this, we separate cells from the background in images by…

Computer Vision and Pattern Recognition · Computer Science 2019-04-02 Lin Zhang

Ptychography promises diffraction limited resolution without the need for high resolution lenses. To achieve high resolution one has to solve the phase problem for many partially overlapping frames. Here we review some of the existing…

Optics · Physics 2012-09-24 C. Yang , J. Qian , A. Schirotzek , F. Maia , S. Marchesini

Ptychography is an imaging technique which involves a sample being illuminated by a coherent, localized probe of illumination. When the probe interacts with the sample, the light is diffracted and a diffraction pattern is detected. Then the…

Numerical Analysis · Mathematics 2023-01-31 Mark Iwen , Michael Perlmutter , Mark Philip Roach

Ptychography is a lensless, computational imaging method that utilises diffraction patterns to determine the amplitude and phase of an object. In transmission ptychography, the diffraction patterns are recorded by a detector positioned…

Image and Video Processing · Electrical Eng. & Systems 2023-01-04 Kira A. M. Maathuis , Jacob Seifert , Allard P. Mosk

We propose and experimentally demonstrate a differential microscopy method to obtain simultaneous amplitude, phase, and quantitative polarization gradient imaging in a single experimental embodiment. A full-field optical spatial…

Optics · Physics 2023-10-11 Jeeban Kumar Nayak , Niladri Modak , Sayan Ghosh , Nirmalya Ghosh

Quantum imaging is an advanced method for microscopy or investigating the optical properties of materials or bio-medical inspections with high accuracy, low noise, and extremely low photo-damage. In previous work, we proposed a quantum…

Optics · Physics 2025-05-09 Mousume Samad , Maki Shimizu , Yasuto Hijikata

A simple method of phase-and-amplitude extraction is derived that corrects for image blurring induced by partially spatially coherent incident illumination using only a single intensity image as input. The method is based on Fresnel…

Optics · Physics 2019-11-19 Mario A. Beltran , David M. Paganin , Daniele Pelliccia

It has been a general trend to develop low-voltage electron microscopes due to their high imaging contrast of the sample and low radiation damage. Atom-resolved transmission electron microscopes with voltages as low as 15-40 kV have been…

Instrumentation and Detectors · Physics 2015-12-29 Wei-Tse Chang , Chun-Yueh Lin , Wei-Hao Hsu , Mu-Tung Chang , Yi-Sheng Chen , En-Te Hwu , Ing-Shouh Hwang

Phase retrieval is a nonlinear inverse problem that arises in a wide range of imaging modalities, from electron microscopy to Fourier ptychography. In particular, the reconstruction is facilitated when the sensing matrix is i.i.d. random,…

Electron tomography is a technique used in both materials science and structural biology to image features well below optical resolution limit. In this work, we present a new algorithm for reconstructing the three-dimensional(3D)…

Signal Processing · Electrical Eng. & Systems 2018-07-12 David Ren , Michael Chen , Laura Waller , Colin Ophus

Sensing with undetected photons allows access to spectral regions with simultaneous detection of photons of another region and is based on nonlinear interferometry. To obtain the full information of a sample, the corresponding interferogram…

Different imaging modalities are used to extract the diverse information carried in an optical field. Two prominent modalities include bright field and phase contrast microscopy that can visualize the amplitude and phase features of a…

Imaging with quantum states of light promises advantages over classical approaches in terms of resolution, signal-to-noise ratio and sensitivity. However, quantum detectors are particularly sensitive sources of classical noise that can…

Quantum Physics · Physics 2019-07-16 Hugo Defienne , Matthew Reichert , Jason W Fleischer , Daniele Faccio

Path-entangled multi-photon states allow optical phase-sensing beyond the shot-noise limit, provided that an efficient parity measurement can be implemented. Realising this experimentally is technologically demanding, as it requires…

In this research, we reveal the inborn but hitherto ignored properties of quantitative differential phase contrast (qDPC) imaging: the phase transfer function being an edge detection filter. Inspired by this, we highlighted the duality of…

Optimization and Control · Mathematics 2023-06-30 Shuhe Zhang , Hao Wu , Tao Peng , Zeyu Ke , Meng Shao , Tos T. J. M. Berendschot , Jinhua Zhou

X-ray ptychography has revolutionised nanoscale phase contrast imaging at large-scale synchrotron sources in recent years. We present here the first successful demonstration of the technique in a small-scale laboratory setting. We conducted…

We present a technically simple implementation of quantitative phase imaging in confocal microscopy based on synthetic optical holography with sinusoidal-phase reference waves. Using a Mirau interference objective and low-amplitude vertical…

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