Related papers: Probing and manipulating embryogenesis via nanosca…
Temperature is one of the most relevant parameters for the regulation of intracellular processes. Measuring localized subcellular temperature gradients is fundamental for a deeper understanding of cell function, such as the genesis of…
Cell division is an essential biological process, and regulation of cell division is of relevance for many important fields of biology and medicine. Introducing exogenous substances, such as nanoparticles, into the nucleus, has been…
Sensitive probing of temperature variations on nanometer scales represents an outstanding challenge in many areas of modern science and technology. In particular, a thermometer capable of sub-degree temperature resolution as well as…
Cell division timing is critical for cell fate specification and morphogenesis during embryogenesis. How division timings are regulated among cells during development is poorly understood. Here we focus on the comparison of asynchrony of…
Viscoelasticity of the cytoplasm plays a critical role in cell morphology and division. In parallel, local temperature is coupled to viscoelasticity and influences cellular bioenergetics. Probing the interdependence of intracellular…
We report a new approach to controllable thermal stimulation of a single living cell and its compartments. The technique is based on the use of a single polycrystalline diamond particle containing silicon-vacancy (SiV) color centers. Due to…
While the thermal and electromagnetic properties of plasmonic nanostructures are well understood, nanoscale thermometry still presents an experimental and theoretical challenge. Plasmonic structures can confine electromagnetic energy at the…
The nematode Caenorhabditis elegans (C. elegans) is used as a model organism to better understand developmental biology and neurobiology. C. elegans features an invariant cell lineage, which has been catalogued and observed using…
Determining cell identities in imaging sequences is an important yet challenging task. The conventional method for cell identification is via cell tracking, which is complex and can be time-consuming. In this study, we propose an innovative…
Real-time temperature monitoring inside living organisms provides a direct measure of their biological activities, such as homeostatic thermoregulation and energy metabolism. However, it is challenging to reduce the size of bio-compatible…
Based on the PubMed database, there are around 260 manuscripts describing nanothermometers. These research articles detail the synthesis, performance, and application of intracellular nanothermometers. This intracellular prevalence is due…
Nanodiamond hosting temperature-sensing centers constitutes a closed thermodynamic system, with the only window of energy exchange with the environment without direct contacts of a sensor with intracellular substrates, which is in fact the…
3D cell tracking in a living organism has a crucial role in live cell image analysis. Cell tracking in C. elegans has two difficulties. First, cell migration in a consecutive frame is large since they move their head during scanning.…
We investigate the intergenerational shape dynamics of single Caulobacter crescentus cells using a novel combination of imaging techniques and theoretical modeling. We determine the dynamics of cell pole-to-pole lengths, cross-sectional…
Biomolecular condensates form on timescales of seconds in cells upon environmental or compositional changes. Condensate formation is thus argued to act as a mechanism for sensing such changes and quickly initiating downstream processes,…
Temperature is a fundamental parameter in the study of physical phenomena. At the nanoscale, local temperature differences can be harnessed to design novel thermal nanoelectronic devices or test quantum thermodynamical concepts. Determining…
Numerical simulations of heat transport in diamond anvil cells reveal a possibility for absolute measurements of specific heat via high-frequency modulation calorimetry. Such experiments could reveal and help characterize temperature-driven…
Across metazoans, early embryos exhibit a strikingly conserved slowing down of their cell duplication speed, despite widely varying developmental paces and underlying molecular mechanisms. Here we show that this common behavior arises…
Tracking the dynamics of fluorescent nanoparticles during embryonic development allows insights into the physical state of the embryo and, potentially, molecular processes governing developmental mechanisms. In this work, we investigate the…
We demonstrate the measurement and manipulation of the temperature of cold CO molecules in a microchip environment. Through the use of time-resolved spatial imaging, we are able to observe the phase-space distribution of the molecules, and…