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Confocal microscopy is the standard approach for obtaining volumetric images of a sample with high axial and lateral resolution, especially when dealing with scattering samples. Unfortunately, a confocal microscope is quite expensive…

Digital holographic microscopy is a fast three-dimensional (3D) imaging tool with many applications in soft matter physics. Recent studies have shown that electromagnetic scattering solutions can be fit to digital holograms to obtain the 3D…

We propose a speed-up method for the in-focus plane detection in digital holographic microscopy that can be applied to a broad class of autofocusing algorithms that involve repetitive propagation of an object wave to various axial locations…

Quantitative Methods · Quantitative Biology 2023-05-19 Julianna Winnik , Damian Suski , Piotr Zdańkowski , Luiza Stanaszek , Vicente Micó , Maciej Trusiak

Single-Molecule Localization Microscopy (SMLM) has expanded our ability to visualize subcellular structures but is limited in its temporal resolution. Increasing emitter density will improve temporal resolution, but current analysis…

Signal Processing · Electrical Eng. & Systems 2023-05-10 Armin Abdehkakha , Craig Snoeyink

Modulation-enhanced localization microscopy (MELM) has demonstrated significant improvements in both lateral and axial localization precision compared to conventional single-molecule localization microscopy (SMLM). However, lateral…

Modern deep learning techniques that regress the relative camera pose between two images have difficulty dealing with challenging scenarios, such as large camera motions resulting in occlusions and significant changes in perspective that…

Computer Vision and Pattern Recognition · Computer Science 2024-12-06 Kefan Chen , Noah Snavely , Ameesh Makadia

Modeling the localized intensive deformation in a damaged solid requires highly refined discretization for accurate prediction, which significantly increases the computational cost. Although adaptive model refinement can be employed for…

Computational Engineering, Finance, and Science · Computer Science 2022-05-18 Jonghyuk Baek , Jiun-Shyan Chen , Kristen Susuki

Microscopy imaging techniques are instrumental for characterization and analysis of biological structures. As these techniques typically render 3D visualization of cells by stacking 2D projections, issues such as out-of-plane excitation and…

Image and Video Processing · Electrical Eng. & Systems 2023-02-16 Amirkoushyar Ziabari , Derek C. Rose , Abbas Shirinifard , David Solecki

Fluoroscopic imaging that captures X-ray images at video framerates is advantageous for guiding catheter insertions by vascular surgeons and interventional radiologists. Visualizing the dynamical movements non-invasively allows complex…

Image and Video Processing · Electrical Eng. & Systems 2020-01-06 Jacky C. K. Chow , Steven K. Boyd , Derek D. Lichti , Janet L. Ronsky

Despite its potential for label-free particle diagnostics, holographic microscopy is limited by specialized processing methods that struggle to generalize across diverse settings. We introduce a deep learning architecture leveraging human…

Optics · Physics 2024-11-26 Shyam Kumar , Jiarong Hong

Detecting and localizing objects in the real 3D space, which plays a crucial role in scene understanding, is particularly challenging given only a single RGB image due to the geometric information loss during imagery projection. We propose…

Computer Vision and Pattern Recognition · Computer Science 2020-04-01 Zengyi Qin , Jinglu Wang , Yan Lu

Accurate segmentation of 3-D cell nuclei in microscopy images is essential for the study of nuclear organization, gene expression, and cell morphodynamics. Current image segmentation methods are challenged by the complexity and variability…

Image and Video Processing · Electrical Eng. & Systems 2022-03-18 Sundaresh Ram , Jeffrey J. Rodriguez

The short and intense pulses of the new X-ray free electron lasers, now operational or under construction, may make possible diffraction experiments on single molecule-sized objects with high resolution, before radiation damage destroys the…

Data Analysis, Statistics and Probability · Physics 2012-04-11 Miklós Tegze , Gábor Bortel

We present a deep learning driven computational approach to overcome the limitations of self-interference digital holography that imposed by inferior axial imaging performances. We demonstrate a 3D deep neural network model can…

Depth information is useful in many image processing applications. However, since taking a picture is a process of projection of a 3D scene onto a 2D imaging sensor, the depth information is embedded in the image. Extracting the depth…

Image and Video Processing · Electrical Eng. & Systems 2021-12-15 Fernando J. Galetto , Guang Deng

An image dataset of 10 different size molecules, where each molecule has 2,000 structural variants, is generated from the 2D cross-sectional projection of Molecular Dynamics trajectories. The purpose of this dataset is to provide a…

Image and Video Processing · Electrical Eng. & Systems 2019-11-19 Yan Zhang , Steve Farrell , Michael Crowley , Lee Makowski , Jack Deslippe

Standard deviation measurements of intensity profiles of stationary single fluorescent molecules are useful for studying axial localization, molecular orientation, and a fluorescence imaging system's spatial resolution. Here we report on…

Quantitative Methods · Quantitative Biology 2015-05-13 Michael C. DeSantis , Shawn H. DeCenzo , Je-Luen Li , Y. M. Wang

Localization has been a challenging task for autonomous navigation. A loop detection algorithm must overcome environmental changes for the place recognition and re-localization of robots. Therefore, deep learning has been extensively…

Robotics · Computer Science 2023-04-19 Alex Junho Lee , Seungwon Song , Hyungtae Lim , Woojoo Lee , Hyun Myung

Computer-aided diagnosis for low-dose computed tomography (CT) based on deep learning has recently attracted attention as a first-line automatic testing tool because of its high accuracy and low radiation exposure. However, existing methods…

Image and Video Processing · Electrical Eng. & Systems 2022-06-28 Kyung-Su Kim , Seong Je Oh , Ju Hwan Lee , Myung Jin Chung

Deep learning (DL) has arguably emerged as the method of choice for the detection and segmentation of biological structures in microscopy images. However, DL typically needs copious amounts of annotated training data that is for biomedical…

Image and Video Processing · Electrical Eng. & Systems 2020-03-20 Mangal Prakash , Tim-Oliver Buchholz , Manan Lalit , Pavel Tomancak , Florian Jug , Alexander Krull