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The classical method of determining the atomic structure of complex molecules by analyzing diffraction patterns is currently undergoing drastic developments. Modern techniques for producing extremely bright and coherent X-ray lasers allow a…

Biomolecules · Quantitative Biology 2015-10-12 Tomas Ekeberg , Stefan Engblom , Jing Liu

The measurements of size distribution of small particles (e.g. dusts, droplets, bubbles, etc.) are critical for a broad range of applications in environmental science, public health, industrial manufacturing, etc. Laser diffraction (LD), a…

Fluid Dynamics · Physics 2021-03-16 Santosh Kumar. S , Zilong He , Christopher Hogan , Steven Fredericks , Jiarong Hong

We introduce a novel framework that incorporates multiple scattering for large-scale 3D particle-localization using single-shot in-line holography. Traditional holographic techniques rely on single-scattering models which become inaccurate…

Signal Processing · Electrical Eng. & Systems 2019-06-19 Waleed Tahir , Ulugbek S. Kamilov , Lei Tian

The key limitations of digital inline holography (DIH) for particle tracking applications are poor longitudinal resolution, particle concentration limits, and case-specific processing. We utilize an inverse problem method with fused lasso…

Image and Video Processing · Electrical Eng. & Systems 2019-07-24 Kevin Mallery , Jiarong Hong

In-line digital holography is a valuable tool for sizing, locating and tracking micro- or nano-objects in a volume. When a parametric imaging model is available, Inverse Problems approaches provide a straightforward estimate of the object…

Instrumentation and Detectors · Physics 2023-07-19 Nicolas Verrier , Corinne Fournier , Thierry Fournel

Polarized light microscopy provides high contrast to birefringent specimen and is widely used as a diagnostic tool in pathology. However, polarization microscopy systems typically operate by analyzing images collected from two or more light…

We demonstrate the fabrication of volume holograms using 2-photon polymerization with dynamic control of light exposure. We refer to our method as (3+1)D printing. Volume holograms that are recorded by interfering reference and signal beams…

Optics · Physics 2023-10-11 Niyazi Ulas Dinc , Christophe Moser , Demetri Psaltis

An automated and reliable processing of bubbly flow images is highly needed to analyse large data sets of comprehensive experimental series. A particular difficulty arises due to overlapping bubble projections in recorded images, which…

Computer Vision and Pattern Recognition · Computer Science 2022-08-08 Hendrik Hessenkemper , Sebastian Starke , Yazan Atassi , Thomas Ziegenhein , Dirk Lucas

Microscopic analysis of micro particles in situ in diverse water environments is necessary for monitoring water quality and localizing contamination sources. Conventional sensors such as optical microscopes and fluorometers often require…

Robotics · Computer Science 2019-11-26 Kevin Mallery , Dario Canelon , Jiarong Hong , Nikolaos Papanikolopoulos

Accurately measuring the size, morphology, and structure of nanoparticles is very important, because they are strongly dependent on their properties for many applications. In this paper, we present a deep-learning based method for…

Materials Science · Physics 2022-07-29 Claudius Zelenka , Marius Kamp , Kolja Strohm , Akram Kadoura , Jacob Johny , Reinhard Koch , Lorenz Kienle

Machine learning-based computer-generated holography (ML-CGH) has advanced rapidly in recent years, yet progress is constrained by the limited availability of high-quality, large-scale hologram datasets. To address this, we present…

Computer Vision and Pattern Recognition · Computer Science 2025-12-25 Jaehong Lee , You Chan No , YoungWoo Kim , Duksu Kim

Understanding the three-dimensional motion of bubbles is essential for interpreting transport and mixing in multiphase flows, especially when bubbles deform under shear or move rapidly through the flow field. In many laboratory setups, only…

Fluid Dynamics · Physics 2026-01-30 Chaitanya S Nayak , Faizaan Mohammed , Vivek Kumar , Shivam Prajapati , Cyrus Aidun

Computer-generated holography (CGH) is a promising method that modulates user-defined waveforms with digital holograms. An efficient and fast pipeline framework is proposed to synthesize CGH using initial point cloud and MRI data. This…

Computer Vision and Pattern Recognition · Computer Science 2025-08-12 Justin London

Far-field characterization of small objects is severely constrained by the diffraction limit. Existing tools achieving sub-diffraction resolution often utilize point-by-point image reconstruction via scanning or labelling. Here, we present…

We demonstrate a smart laser-diffraction analysis technique for particle mixture identification. We retrieve information about the size, geometry, and ratio concentration of two-component heterogeneous particle mixtures with an efficiency…

Image and Video Processing · Electrical Eng. & Systems 2022-02-03 Arturo Villegas , Mario A. Quiroz-Juarez , Alfred U'Ren , Juan P. Torres , Roberto de J. Leon-Montiel

We present a new technique that combines off-axis Digital Holography and Dark Field Microscopy to track 100nm gold particles diffusing in water. We show that a single hologram is sufficient to localize several particles in a thick sample…

Optics · Physics 2015-06-03 Frédéric Verpillat , Fadwa Joud , Pierre Desbiolles , Michel Gross

Three-dimensional particle reconstruction with limited two-dimensional projections is an under-determined inverse problem that the exact solution is often difficult to be obtained. In general, approximate solutions can be obtained by…

Image and Video Processing · Electrical Eng. & Systems 2021-09-14 Qi Gao , Shaowu Pan , Hongping Wang , Runjie Wei , Jinjun Wang

Colloidoscope is a deep learning pipeline employing a 3D residual Unet architecture, designed to enhance the tracking of dense colloidal suspensions through confocal microscopy. This methodology uses a simulated training dataset that…

Controlling crystalline material defects is crucial, as they affect properties of the material that may be detrimental or beneficial for the final performance of a device. Defect analysis on the sub-nanometer scale is enabled by…

Materials Science · Physics 2021-06-03 Nik Dennler , Antonio Foncubierta-Rodriguez , Titus Neupert , Marilyne Sousa

Off-axis digital holographic microscopy is a high-throughput, label-free imaging technology that provides three-dimensional, high-resolution information about samples, particularly useful in large-scale cellular imaging. However, the…