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The role and importance of mechanical properties of cells and tissues in cellular function, development as well as disease has widely been acknowledged, however standard techniques currently used to assess them exhibit intrinsic…

Biological Physics · Physics 2019-01-09 Robert Prevedel , Alba Diz-Muñoz , Giancarlo Ruocco , Giuseppe Antonacci

Characterizing the micromechanical properties of cells and extracellular matrices is critical in mechanobiology. To meet this need, Brillouin light scattering (BLS) has emerged as a noncontact, high-resolution elastography tool that probes…

Hydrogels are attractive and versatile gel-based structures consisting of three-dimensional networks of hydrophilic polymers with unique properties that can retain a large amount of water and be tailor-designed according to specific…

In this study, Brillouin spectroscopy was employed to investigate the viscoelastic properties of melanoma cells in vitro. Using a custom-built confocal Brillouin microspectrometer, we obtained Brillouin shifts and full width at half maximum…

Brillouin spectroscopy and imaging are emerging techniques in analytical science, biophotonics and biomedicine. They are based on Brillouin light scattering from acoustic waves or phonons in the GHz range, providing a nondestructive…

Biological Physics · Physics 2021-10-12 Francesca Palombo , Daniele Fioretto

Brillouin microscopy is an emerging optical elastography technique that can be used to assess mechanical properties of biological samples in a 3D, all-optical and hence non-contact fashion. However, the low cross-section of spontaneous…

Optics · Physics 2024-09-04 Carlo Bevilacqua , Robert Prevedel

Brillouin imaging is increasingly recognized to be a powerful technique that enables non-invasive measurement of the mechanical properties of cells and tissues on a microscopic scale. This provides an unprecedented tool for investigating…

Biological Physics · Physics 2020-01-22 Christine Poon , Joshua Chou , Michael Cortie , Irina Kabakova

The biomechanical properties of cells and tissues play an important role in our fundamental understanding of the structures and functions of biological systems at both the cellular and subcellular levels. Recently, Brillouin microscopy,…

Brillouin light scattering spectroscopy (BLS) is applied to study the micromechanics of cellulosic viscose fibers, one of the commercially most important, man-made biobased fibers. Using an equal angle scattering geometry, we provide a…

Brillouin Light Scattering is a powerful technique to measure the microwave excitations present in a magnetic system. In microfocused mode, the light is focused on the sample using a microscope objective. This accelerates substantially the…

Mesoscale and Nanoscale Physics · Physics 2025-06-30 Nessrine Benaziz , Thibaut Devolder , Jean-Paul Adam

Brillouin microscopy is an emerging technique for all-optical biomechanical imaging without the need for physical contact with the sample or for an external mechanical stimulus. However, Brillouin microscopy often retrieves a single,…

Optics · Physics 2024-02-27 Roni Shaashoua , Tal Levy , Barak Rotblat , Alberto Bilenca

Brillouin imaging is an emerging optical elastography technique able to generate maps of the mechanical properties at microscale, with great potential in biophysical and biomedical fields. A key parameter is its spatial resolution, which is…

Optics · Physics 2020-02-28 Silvia Caponi , Daniele Fioretto , Maurizio Mattarelli

Brillouin spectroscopy is a century-old technique that has recently received renewed interest, as modern instrumentation has transformed it into a powerful contactless and label-free probe of micromechanical properties for biomedical…

Instrumentation and Detectors · Physics 2020-05-27 YuChen Xiang , Carin Basirun , Joshua Chou , Majid E. Warkiani , Peter Török , Yingying Wang , Shoufei Gao , Irina V. Kabakova

Brillouin microscopy measures compressibility, but is being increasingly used to assess stiffness of cells and tissues. Using hydrogels with tunable properties, we demonstrate that Brillouin microscopy is insensitive to stiffness of…

Quantitative Methods · Quantitative Biology 2018-11-30 Pei-Jung Wu , Irina Kabakova , Jeffrey Ruberti , Joseph M. Sherwood , Iain E. Dunlop , Carl Paterson , Peter Török , Darryl R. Overby

Brillouin spectroscopy was used to probe the viscoelastic properties of E. coli bacterial cell lysate in aqueous solution at GHz-frequencies over the range -5.0 $^\circ$C $\leq T \leq$ 50.0 $^\circ$C. This work offers a first temperature…

Soft Condensed Matter · Physics 2023-11-13 D. F. Hanlon , S. Heravi , V. Booth , G. T. Andrews

There is currently a renewed interest in the development of experimental methods to achieve the elastic characterization of thin films, multilayers and acoustic resonators operating in the GHz range of frequencies. The potentialities of…

Applied Physics · Physics 2019-08-30 Giovanni Carlotti

Brillouin scattering has applications ranging from signal processing, sensing and microscopy, to quantum information and fundamental science. Most of these applications rely on the electrostrictive interaction between light and phonons.…

Brillouin microscopy is an emerging label-free imaging technique to assess local viscoelastic properties. Quantum-enhanced stimulated Brillouin scattering is demonstrated for the first time using low power continuous-wave lasers at 795~nm.…

Optics · Physics 2024-09-16 Tian Li , Fu Li , Xinghua Liu , Vladislav V. Yakovlev , Girish S. Agarwal

Brillouin scattering has been used to measure the dynamic structure factor of glycerol as a function of temperature from the high temperature liquid to the glassy state. Our investigation aims at understanding the number and the nature of…

Soft Condensed Matter · Physics 2009-11-10 Lucia Comez , Daniele Fioretto , Filippo Scarponi , Giulio Monaco

Brillouin scattering has been widely exploited for advanced photonics functionalities such as microwave photonics, signal processing, sensing, lasing, and more recently in micro- and nano-photonic waveguides. So far, all the works have…

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