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The accurate segmentation and tracking of cells in microscopy image sequences is an important task in biomedical research, e.g., for studying the development of tissues, organs or entire organisms. However, the segmentation of touching…

Computer Vision and Pattern Recognition · Computer Science 2020-12-10 Tim Scherr , Katharina Löffler , Moritz Böhland , Ralf Mikut

The full understanding of cellular functions requires information about protein numbers for various biomolecular assemblies and their dynamics, which can be partly accessed by super-resolution fluorescence microscopy. Yet, many protein…

Biological Physics · Physics 2020-06-17 Kristin S. Grußmayer , Klaus Yserentant , Dirk-Peter Herten

Quantifying the size of cell populations is crucial for understanding biological processes such as growth, injury repair, and disease progression. Often, experimental data offer information in the form of relative frequencies of distinct…

Quantitative Methods · Quantitative Biology 2024-05-09 Yuman Wang , Shuli Chen , Jie Hu , Da Zhou

Cells are the fundamental unit of biological organization, and identifying them in imaging data - cell segmentation - is a critical task for various cellular imaging experiments. While deep learning methods have led to substantial progress…

Phase contrast techniques, such as differential interference contrast (DIC) microscopy, are widely used to provide morphological images of unstained biological samples. The trans-illumination geometry required for these techniques has…

Optics · Physics 2015-03-13 Tim N. Ford , Kengyeh K. Chu , Jerome Mertz

In systems biomedicine, an experimenter encounters different potential sources of variation in data such as individual samples, multiple experimental conditions, and multi-variable network-level responses. In multiparametric cytometry,…

Cell segmentation is a fundamental task in microscopy image analysis. Several foundation models for cell segmentation have been introduced, virtually all of them are extensions of Segment Anything Model (SAM), improving it for microscopy…

Computer Vision and Pattern Recognition · Computer Science 2026-03-19 Anwai Archit , Constantin Pape

Conventional cell tracking methods detect multiple cells in each frame (detection) and then associate the detection results in successive time-frames (association). Most cell tracking methods perform the association task independently from…

Computer Vision and Pattern Recognition · Computer Science 2020-02-27 Junya Hayashida , Kazuya Nishimura , Ryoma Bise

Imaging flow cytometry shows significant potential for increasing our understanding of heterogeneous and complex life systems and is useful for biomedical applications. Ghost cytometry is a recently proposed approach for directly analyzing…

The progress in imaging techniques have allowed the study of various aspect of cellular mechanisms. To isolate individual cells in live imaging data, we introduce an elegant image segmentation framework that effectively extracts cell…

Computer Vision and Pattern Recognition · Computer Science 2016-02-18 Arnaud Browet , Christophe De Vleeschouwer , Laurent Jacques , Navrita Mathiah , Bechara Saykali , Isabelle Migeotte

Cell biologists study in parallel the morphology of cells with the regulation mechanisms that modify this morphology. Such studies are complicated by the inherent heterogeneity present in the cell population. It remains difficult to define…

Quantitative Methods · Quantitative Biology 2025-12-23 Yossi Bokor Bleile , Pooja Yadav , Patrice Koehl , Florian Rehfeldt

In this paper, we review spatial light interference microscopy (SLIM), a common-path, phase-shifting interferometer, built onto a phase-contrast microscope, with white-light illumination. As one of the most sensitive quantitative phase…

Optics · Physics 2020-12-17 Xi Chen , Mikhail E. Kandel , Gabriel Popescu

Medical image segmentation is a crucial task in medical image analysis, but it can be very challenging especially when there are less labeled data but with large unlabeled data. Contrastive learning has proven to be effective for medical…

Computer Vision and Pattern Recognition · Computer Science 2025-02-11 Shihuan He , Zhihui Lai , Ruxin Wang , Heng Kong

Cellphones equipped with high-quality cameras and powerful CPUs as well as GPUs are widespread. This opens new prospects to use such existing computational and imaging resources to perform medical diagnosis in developing countries at a very…

Light and matter share fundamental statistical properties, yet the experimental probes of quantum optics and many-body physics have largely evolved along separate trajectories. While many-body physics explores emergent collective phenomena,…

The spread of microbial infections is governed by the self-organization of bacteria on surfaces. Limitations of live imaging techniques make collective behaviors in clinically relevant systems challenging to quantify. Here, novel…

Biological Physics · Physics 2024-07-19 Vincent Hickl , Abid Khan , René M. Rossi , Bruno F. B. Silva , Katharina Maniura-Weber

For measuring the strength of visually-observed subpopulation differences, the Population Difference Criterion is proposed to assess the statistical significance of visually observed subpopulation differences. It addresses the following…

Methodology · Statistics 2023-09-21 Xi Yang , Jan Hannig , Katherine A. Hoadley , Iain Carmichael , J. S. Marron

In medicine, visualizing chromosomes is important for medical diagnostics, drug development, and biomedical research. Unfortunately, chromosomes often overlap and it is necessary to identify and distinguish between the overlapping…

Computer Vision and Pattern Recognition · Computer Science 2017-12-21 R. Lily Hu , Jeremy Karnowski , Ross Fadely , Jean-Patrick Pommier

Contrast pattern mining (CPM) aims to discover patterns whose support increases significantly from a background dataset compared to a target dataset. CPM is particularly useful for characterising changes in evolving systems, e.g., in…

Networking and Internet Architecture · Computer Science 2020-12-01 Elaheh AlipourChavary , Sarah M. Erfani , Christopher Leckie

In recent years, several unsupervised cell segmentation methods have been presented, trying to omit the requirement of laborious pixel-level annotations for the training of a cell segmentation model. Most if not all of these methods handle…

Computer Vision and Pattern Recognition · Computer Science 2023-12-04 Mehdi Naouar , Gabriel Kalweit , Anusha Klett , Yannick Vogt , Paula Silvestrini , Diana Laura Infante Ramirez , Roland Mertelsmann , Joschka Boedecker , Maria Kalweit