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In cryo-electron microscopy (EM), molecular structures are determined from large numbers of projection images of individual particles. To harness the full power of this single-molecule information, we use the Bayesian inference of EM…

Biomolecules · Quantitative Biology 2018-01-17 Pilar Cossio , David Rohr , Fabio Baruffa , Markus Rampp , Volker Lindenstruth , Gerhard Hummer

The cryo-electron microscopy (Cryo-EM) becomes popular for macromolecular structure determination. However, the 2D images which Cryo-EM detects are of high noise and often mixed with multiple heterogeneous conformations or contamination,…

Image and Video Processing · Electrical Eng. & Systems 2022-02-22 Hanlin Gu , Yin Xian , Ilona Christy Unarta , Yuan Yao

A common task in cryo-electron microscopy (cryo-EM) data processing is to compare three-dimensional density maps of macromolecules. In this paper, we propose an algorithm for aligning three-dimensional density maps that exploits common…

Image and Video Processing · Electrical Eng. & Systems 2023-02-27 Yael Harpaz , Yoel Shkolnisky

Machine learning-based interatomic potentials and force fields depend critically on accurate atomic structures, yet such data are scarce due to the limited availability of experimentally resolved crystals. Although atomic-resolution…

Computer Vision and Pattern Recognition · Computer Science 2025-05-20 Yaotian Yang , Yiwen Tang , Yizhe Chen , Xiao Chen , Jiangjie Qiu , Hao Xiong , Haoyu Yin , Zhiyao Luo , Yifei Zhang , Sijia Tao , Wentao Li , Qinghua Zhang , Yuqiang Li , Wanli Ouyang , Bin Zhao , Xiaonan Wang , Fei Wei

Structural flexibility and/or dynamic interactions with other molecules is a critical aspect of protein function. CryoEM provides direct visualization of individual macromolecules sampling different conformational and compositional states.…

Biomolecules · Quantitative Biology 2021-08-04 Muyuan Chen , Steven Ludtke

The exotic internal structure of polar topologies in multi-ferroic materials offers a rich landscape for materials science research. As the spatial scale of these entities are often sub-atomic in nature, aberration corrected transmission…

Cryo-electron tomography (cryo-ET) enables high resolution, three-dimensional reconstruction of biological structures, including membranes and membrane proteins. Identification of regions of interest (ROIs) is central to scientific imaging,…

Computer Vision and Pattern Recognition · Computer Science 2026-02-25 Xingyi Cheng , Julien Maufront , Aurélie Di Cicco , Daniël M. Pelt , Manuela Dezi , Daniel Lévy

Learning robust representations of 3D shapes from voxelized data is essential for advancing AI methods in biomedical imaging. However, most contemporary 3D computer vision approaches operate on point clouds, meshes, or octrees, while…

Image and Video Processing · Electrical Eng. & Systems 2026-03-05 Rui Li , Artsemi Yushkevich , Mikhail Kudryashev , Artur Yakimovich

Cryo-Electron Microscopy (cryo-EM) has become an extremely powerful method for resolving structural details of large biomolecular complexes. However, challenging problems in single-particle methods remain open because of (1) the low…

Biomolecules · Quantitative Biology 2019-08-12 Shengnan Lyu , Christian Wülker , Yuqing Pan , Amitesh S. Jayaraman , Jianhao Zheng , Yilin Cai , Gregory S. Chirikjian

Multiple approaches to use deep learning for image restoration have recently been proposed. Training such approaches requires well registered pairs of high and low quality images. While this is easily achievable for many imaging modalities,…

Computer Vision and Pattern Recognition · Computer Science 2024-10-30 Tim-Oliver Buchholz , Mareike Jordan , Gaia Pigino , Florian Jug

Cryo-electron microscopy (cryo-EM) is an experimental technique for protein structure determination that images an ensemble of macromolecules in near-physiological contexts. While recent advances enable the reconstruction of dynamic…

Cryo-electron microscopy (cryo-EM) has emerged as a powerful technique for resolving the three-dimensional structures of macromolecules. A key challenge in cryo-EM is characterizing continuous heterogeneity, where molecules adopt a…

Machine Learning · Statistics 2026-02-20 Roey Yadgar , Roy R. Lederman , Yoel Shkolnisky

Clinicians are interested in better understanding complex diseases, such as cancer or rare diseases, so they need to produce and exchange data to mutualize sources and join forces. To do so and ensure privacy, a natural way consists in…

Databases · Computer Science 2025-09-29 Nelly Barret , Anna Bernasconi , Boris Bikbov , Pietro Pinoli

Pose estimation from unordered images is fundamental for 3D reconstruction, robotics, and scientific imaging. Recent geometric foundation models, such as DUSt3R, enable end-to-end dense 3D reconstruction but remain underexplored in…

Computer Vision and Pattern Recognition · Computer Science 2025-10-15 Jiakai Zhang , Shouchen Zhou , Haizhao Dai , Xinhang Liu , Peihao Wang , Zhiwen Fan , Yuan Pei , Jingyi Yu

Due to the extremely low signal-to-noise ratio (SNR) and unknown poses (projection angles and image shifts) in cryo-electron microscopy (cryo-EM) experiments, reconstructing 3D volumes from 2D images is very challenging. In addition to…

Image and Video Processing · Electrical Eng. & Systems 2024-04-24 Weijie Chen , Yuhang Wang , Lin Yao

Resolving the structural variability of proteins is often key to understanding the structure-function relationship of those macromolecular machines. Single particle analysis using Cryogenic electron microscopy (CryoEM), combined with…

Quantitative Methods · Quantitative Biology 2022-11-22 Muyuan Chen , Bogdan Toader , Roy Lederman

The goal of cryo-electron microscopy (EM) is to reconstruct the 3-dimensional structure of a molecule from a collection of its 2-dimensional projected images. In this article, we show that the basic premise of cryo-EM --- patching together…

Computer Vision and Pattern Recognition · Computer Science 2017-04-25 Ke Ye , Lek-Heng Lim

Background: Single-particle cryo-electron microscopy (cryo-EM) has become a popular tool for structural determination of biological macromolecular complexes. High-resolution cryo-EM reconstruction often requires hundreds of thousands of…

Data Analysis, Statistics and Probability · Physics 2017-09-05 Yanan Zhu , Qi Ouyang , Youdong Mao

Electrical impedance tomography (EIT) is a non-invasive imaging technique, capable of reconstructing images of the electrical conductivity of tissues and materials. It is popular in diverse application areas, from medical imaging to…

Computer Vision and Pattern Recognition · Computer Science 2024-12-24 Shuaikai Shi , Ruiyuan Kang , Panos Liatsis

Aberration-corrected scanning electron microscopy (AC-STEM) can provide valuable information on the atomic structure of nanoclusters, an essential input for gaining an understanding of their physical and chemical properties. A systematic…

Materials Science · Physics 2021-05-11 Kusse S. Bersha , Alejandro Peña-Torres , Hannes Jónsson