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Label-free tomographic microscopy offers a compelling means to visualize three-dimensional (3D) refractive index (RI) distributions from two-dimensional (2D) intensity measurements. However, limited forward-model accuracy and the ill-posed…

Optics · Physics 2025-02-27 Delong Yang , Shaohui Zhang , Jiasong Sun , Chao Zuo , Qun Hao

At the present time Optical Coherence Tomography (OCT) is among the most commonly used non-invasive imaging methods for the acquisition of large volumetric scans of human retinal tissues and vasculature. To resolve decisive information from…

Computer Vision and Pattern Recognition · Computer Science 2021-11-02 D. Sitenko , B. Boll , C. Schnörr

Optical coherence tomography (OCT) is one of the non-invasive and easy-to-acquire biomarkers (the thickness of the retinal layers, which is detectable within OCT scans) being investigated to diagnose Alzheimer's disease (AD). This work aims…

Image and Video Processing · Electrical Eng. & Systems 2022-06-14 Paria Jeihouni , Omid Dehzangi , Annahita Amireskandari , Ali Dabouei , Ali Rezai , Nasser M. Nasrabadi

In optical diffraction tomography (ODT), the three-dimensional scattering potential of a microscopic object rotating around its center is recovered by a series of illuminations with coherent light. Reconstruction algorithms such as the…

Numerical Analysis · Mathematics 2022-09-02 Robert Beinert , Michael Quellmalz

We demonstrate a label-free, scan-free {\it intensity} diffraction tomography technique utilizing annular illumination (aIDT) to rapidly characterize large-volume 3D refractive index distributions in vitro. By optimally matching the…

Optics · Physics 2020-01-01 Jiaji Li , Alex Matlock , Yunzhe Li , Qian Chen , Chao Zuo , Lei Tian

High anisotropy in volumetric medical images can lead to the inconsistent quantification of anatomical and pathological structures. Particularly in optical coherence tomography (OCT), slice spacing can substantially vary across and within…

Image and Video Processing · Electrical Eng. & Systems 2024-10-15 Coen de Vente , Mohammad Mohaiminul Islam , Philippe Valmaggia , Carel Hoyng , Adnan Tufail , Clara I. Sánchez

The characterization of white blood cells (WBCs) is crucial for blood analyses and disease diagnoses. However, current standard techniques rely on cell labeling, a process which imposes significant limitations. Here we present…

Biological Physics · Physics 2015-05-12 Jonghee Yoon , Kyoohyun Kim , HyunJoo Park , Chulhee Choi , Seongsoo Jang , YongKeun Park

Multispectral image analysis is a relatively promising field of research with applications in several areas, such as medical imaging and satellite monitoring. A considerable number of current methods of analysis are based on parametric…

Deep Learning (DL) models tend to perform poorly when the data comes from a distribution different from the training one. In critical applications such as medical imaging, out-of-distribution (OOD) detection helps to identify such data…

Image and Video Processing · Electrical Eng. & Systems 2023-06-26 Daria Frolova , Anton Vasiliuk , Mikhail Belyaev , Boris Shirokikh

Optical coherence tomography (OCT) is a volumetric imaging modality that empowers clinicians and scientists to noninvasively visualize the cross-sections of biological samples. As the latest generation of its kind, Fourier-domain OCT…

Image and Video Processing · Electrical Eng. & Systems 2020-05-20 Yuye Ling , Mengyuan Wang , Yu Gan , Xinwen Yao , Leopold Schmetterer , Chuanqing Zhou , Yikai Su

Dynamic optical coherence tomography (DOCT) statistically analyzes fluctuations in time-sequential OCT signals, enabling label-free and three-dimensional visualization of intratissue and intracellular activities. Current DOCT methods, such…

Optical coherence tomography (OCT) captures cross-sectional data and is used for the screening, monitoring, and treatment planning of retinal diseases. Technological developments to increase the speed of acquisition often results in systems…

Image and Video Processing · Electrical Eng. & Systems 2023-01-03 Timothy T. Yu , Da Ma , Jayden Cole , Myeong Jin Ju , Mirza F. Beg , Marinko V. Sarunic

Three-dimensional molecular imaging of living cells is essential for unraveling cellular metabolism and response to therapies. However, existing volumetric methods, including fluorescence microscopy and quantitative phase imaging, either…

The evaluation and monitoring of cells health in the human retina is crucial and follow time course of retinal diseases, detect lesions before irreversible visual loss and to evaluate treatment effects. Towards this goal, a major challenge…

While optical coherence tomography (OCT) provides a resolution down to 1 micrometer it has difficulties to visualize cellular structures due to a lack of scattering contrast. By evaluating signal fluctuations, a significant contrast…

Optical tomographic cross-sectional images of biological samples were made possible by interferometric imaging techniques such as Optical Coherence Tomography (OCT). Owing to its unprecedented view of the sample, OCT has become a gold…

Medical Physics · Physics 2020-07-13 Pedro Mecê , Elena Gofas-Salas , Michel Paques , Kate Grieve , Serge Meimon

We present a tomographic imaging technique, termed Deep Prior Diffraction Tomography (DP-DT), to reconstruct the 3D refractive index (RI) of thick biological samples at high resolution from a sequence of low-resolution images collected…

Image and Video Processing · Electrical Eng. & Systems 2021-11-18 Kevin C. Zhou , Roarke Horstmeyer

Lipid droplets (LDs) are subcellular organelles with important roles in lipid storage and metabolism and involved in various diseases including cancer, obesity, and diabetes. Conventional methods, however, have limited ability to provide…

Biological Physics · Physics 2016-11-08 Kyoohyun Kim , SeoEun Lee , Jonghee Yoon , JiHan Heo , Chulhee Choi , YongKeun Park

Modern biomedical image analysis using deep learning often encounters the challenge of limited annotated data. To overcome this issue, deep generative models can be employed to synthesize realistic biomedical images. In this regard, we…

Image and Video Processing · Electrical Eng. & Systems 2026-02-23 Yuli Wu , Weidong He , Dennis Eschweiler , Ningxin Dou , Zixin Fan , Shengli Mi , Peter Walter , Johannes Stegmaier

Optical coherence tomography angiography (OCTA) is a non-invasive imaging modality that can acquire high-resolution volumes of the retinal vasculature and aid the diagnosis of ocular, neurological and cardiac diseases. Segmenting the…