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Semantic segmentation constitutes an integral part of medical image analyses for which breakthroughs in the field of deep learning were of high relevance. The large number of trainable parameters of deep neural networks however renders them…

Computer Vision and Pattern Recognition · Computer Science 2017-02-28 Simon Kohl , David Bonekamp , Heinz-Peter Schlemmer , Kaneschka Yaqubi , Markus Hohenfellner , Boris Hadaschik , Jan-Philipp Radtke , Klaus Maier-Hein

Gram staining has been one of the most frequently used staining protocols in microbiology for over a century, utilized across various fields, including diagnostics, food safety, and environmental monitoring. Its manual procedures make it…

Due to image blurring image deconvolution is often used for studying biological structures in fluorescence microscopy. Fluorescence microscopy image volumes inherently suffer from intensity inhomogeneity, blur, and are corrupted by various…

Computer Vision and Pattern Recognition · Computer Science 2019-07-15 Soonam Lee , Shuo Han , Paul Salama , Kenneth W. Dunn , Edward J. Delp

Recent advances in cancer research largely rely on new developments in microscopic or molecular profiling techniques offering high level of detail with respect to either spatial or molecular features, but usually not both. Here, we present…

In an effort to explore high-throughput processing of microscopic image data, a method based on deep convolutional neural network is proposed. The state-of-the-art computer vision algorithm, Faster R-CNN, was trained for the detection of…

Disordered Systems and Neural Networks · Physics 2021-03-09 Ze-Bin Wu

Although generative adversarial networks (GANs) have shown promise in medical imaging, they have four main limitations that impeded their utility: computational cost, data requirements, reliable evaluation measures, and training complexity.…

The rise of automation and machine learning (ML) in electron microscopy has the potential to revolutionize materials research through autonomous data collection and processing. A significant challenge lies in developing ML models that…

Materials Science · Physics 2023-05-31 Abid Khan , Chia-Hao Lee , Pinshane Y. Huang , Bryan K. Clark

Deep learning in medical imaging faces obstacles: limited data diversity, ethical issues, high acquisition costs, and the need for precise annotations. Bleeding detection and localization during surgery is especially challenging due to the…

Image and Video Processing · Electrical Eng. & Systems 2025-06-18 Niran Nataraj , Maina Sogabe , Kenji Kawashima

Immunofluorescent (IF) imaging is crucial for visualizing biomarker expressions, cell morphology and assessing the effects of drug treatments on sub-cellular components. IF imaging needs extra staining process and often requiring cell…

Accurately counting the number of cells in microscopy images is required in many medical diagnosis and biological studies. This task is tedious, time-consuming, and prone to subjective errors. However, designing automatic counting methods…

Image and Video Processing · Electrical Eng. & Systems 2020-11-11 Shenghua He , Kyaw Thu Minn , Lilianna Solnica-Krezel , Mark A. Anastasio , Hua Li

Despite deep convolutional neural networks boost the performance of image classification and segmentation in digital pathology analysis, they are usually weak in interpretability for clinical applications or require heavy annotations to…

Computer Vision and Pattern Recognition · Computer Science 2020-02-10 Yongxiang Huang , Albert C. S. Chung

Localization of anatomical landmarks is essential for clinical diagnosis, treatment planning, and research. In this paper, we propose a novel deep network, named feature aggregation and refinement network (FARNet), for the automatic…

Computer Vision and Pattern Recognition · Computer Science 2021-11-02 Yueyuan Ao , Hong Wu

The application of supervised deep learning methods in digital pathology is limited due to their sensitivity to domain shift. Digital Pathology is an area prone to high variability due to many sources, including the common practice of…

Image and Video Processing · Electrical Eng. & Systems 2020-12-24 Jelica Vasiljević , Friedrich Feuerhake , Cédric Wemmert , Thomas Lampert

Scanning X-ray nanodiffraction microscopy is a powerful technique for spatially resolving nanoscale structural morphologies by diffraction contrast. One of the critical challenges in experimental nanodiffraction data analysis is posed by…

Applied Physics · Physics 2024-06-26 Aileen Luo , Tao Zhou , Martin V. Holt , Andrej Singer , Mathew J. Cherukara

Fluorescence lifetime imaging microscopy (FLIM) is a powerful quantitative technique that provides metabolic and molecular contrast, offering strong translational potential for label-free, real-time diagnostics. However, its clinical…

Computer Vision and Pattern Recognition · Computer Science 2025-12-19 Paloma Casteleiro Costa , Parnian Ghapandar Kashani , Xuhui Liu , Alexander Chen , Ary Portes , Julien Bec , Laura Marcu , Aydogan Ozcan

Cell classification and counting in immunohistochemical cytoplasm staining images play a pivotal role in cancer diagnosis. Weakly supervised learning is a potential method to deal with labor-intensive labeling. However, the inconstant cell…

Image and Video Processing · Electrical Eng. & Systems 2022-03-01 Shichuan Zhang , Chenglu Zhu , Honglin Li , Jiatong Cai , Lin Yang

Imaging assays of cellular function, especially those using fluorescent stains, are ubiquitous in the biological and medical sciences. Despite advances in computer vision, such images are often analyzed using only manual or rudimentary…

Computer Vision and Pattern Recognition · Computer Science 2017-06-06 Lena R. Bartell , Lawrence J. Bonassar , Itai Cohen

Deep learning methods can be found in many medical imaging applications. Recently, those methods were applied directly to the RF ultrasound multi-channel data to enhance the quality of the reconstructed images. In this paper, we apply a…

Signal Processing · Electrical Eng. & Systems 2020-11-23 Nissim Peretz , Arie Feuer

Application of deep learning in digital pathology shows promise on improving disease diagnosis and understanding. We present a deep generative model that learns to simulate high-fidelity cancer tissue images while mapping the real images…

Image and Video Processing · Electrical Eng. & Systems 2020-04-15 Adalberto Claudio Quiros , Roderick Murray-Smith , Ke Yuan

Histochemical staining is essential for visualizing tissue architecture and cellular morphology but is destructive and limited by the availability of tissue for multiple stains. Virtual staining with label-free microscopy offers a…

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