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In addition to evaluating tumor morphology using H&E staining, immunohistochemistry is used to assess the presence of specific proteins within the tissue. However, this is a costly and labor-intensive technique, for which virtual staining,…

Computer Vision and Pattern Recognition · Computer Science 2025-11-25 José Teixeira , Pascal Klöckner , Diana Montezuma , Melis Erdal Cesur , João Fraga , Hugo M. Horlings , Jaime S. Cardoso , Sara P. Oliveira

Manual counting of mitotic tumor cells in tissue sections constitutes one of the strongest prognostic markers for breast cancer. This procedure, however, is time-consuming and error-prone. We developed a method to automatically detect…

The deployment of computer-aided diagnosis systems for cervical cancer screening using whole slide images (WSIs) faces critical challenges due to domain shifts caused by staining variations across different scanners and imaging…

Image and Video Processing · Electrical Eng. & Systems 2025-03-11 Jiangdong Cai , Haotian Jiang , Zhenrong Shen , Yonghao Li , Honglin Xiong , Lichi Zhang , Qian Wang

Multiple Instance Learning (MIL) has been widely applied in histopathology to classify Whole Slide Images (WSIs) with slide-level diagnoses. While the ground truth is established by expert pathologists, the slides can be difficult to…

Computer Vision and Pattern Recognition · Computer Science 2026-03-11 Marie Arrivat , Rémy Peyret , Elsa Angelini , Pietro Gori

Molecular testing of tumor samples for targetable biomarkers is restricted by a lack of standardization, turnaround-time, cost, and tissue availability across cancer types. Additionally, targetable alterations of low prevalence may not be…

Computer Vision and Pattern Recognition · Computer Science 2024-07-23 Kshitij Ingale , Sun Hae Hong , Qiyuan Hu , Renyu Zhang , Bo Osinski , Mina Khoshdeli , Josh Och , Kunal Nagpal , Martin C. Stumpe , Rohan P. Joshi

In digital pathology, many image analysis tasks are challenged by the need for large and time-consuming manual data annotations to cope with various sources of variability in the image domain. Unsupervised domain adaptation based on…

Image and Video Processing · Electrical Eng. & Systems 2022-05-18 Nassim Bouteldja , Barbara Mara Klinkhammer , Tarek Schlaich , Peter Boor , Dorit Merhof

Whole-slide images (WSIs) are critical for cancer diagnosis due to their ultra-high resolution and rich semantic content. However, their massive size and the limited availability of fine-grained annotations pose substantial challenges for…

Computer Vision and Pattern Recognition · Computer Science 2025-06-30 Daoxi Cao , Hangbei Cheng , Yijin Li , Ruolin Zhou , Xuehan Zhang , Xinyi Li , Binwei Li , Xuancheng Gu , Jianan Zhang , Xueyu Liu , Yongfei Wu

It has been shown that unpaired image-to-image translation methods constrained by cycle-consistency hide the information necessary for accurate input reconstruction as imperceptible noise. We demonstrate that, when applied to histopathology…

Computer Vision and Pattern Recognition · Computer Science 2021-03-23 Jelica Vasiljević , Friedrich Feuerhake , Cédric Wemmert , Thomas Lampert

Style transfer is a field with growing interest and use cases in deep learning. Recent work has shown Generative Adversarial Networks(GANs) can be used to create realistic images of virtually stained slide images in digital pathology with…

Computer Vision and Pattern Recognition · Computer Science 2019-06-04 Amal Lahiani , Nassir Navab , Shadi Albarqouni , Eldad Klaiman

Tumor spatial heterogeneity analysis requires precise correlation between Hematoxylin and Eosin H&E morphology and immunohistochemical (IHC) biomarker expression, yet current methods suffer from spatial misalignment in consecutive sections,…

Computer Vision and Pattern Recognition · Computer Science 2025-09-03 Yizhe Yuan , Bingsen Xue , Bangzheng Pu , Chengxiang Wang , Cheng Jin

Manually annotating nuclei from the gigapixel Hematoxylin and Eosin (H&E)-stained Whole Slide Images (WSIs) is a laborious and costly task, meaning automated algorithms for cell nuclei instance segmentation and classification could…

Computer Vision and Pattern Recognition · Computer Science 2024-07-29 João D. Nunes , Diana Montezuma , Domingos Oliveira , Tania Pereira , Jaime S. Cardoso

The automated analysis of medical images is currently limited by technical and biological noise and bias. The same source tissue can be represented by vastly different images if the image acquisition or processing protocols vary. For an…

Self-supervised learning (SSL) enables learning useful inductive biases through utilizing pretext tasks that require no labels. The unlabeled nature of SSL makes it especially important for whole slide histopathological images (WSIs), where…

Computer Vision and Pattern Recognition · Computer Science 2022-11-15 Wisdom Oluchi Ikezogwo , Mehmet Saygin Seyfioglu , Linda Shapiro

In computational pathology, deep learning (DL) models for tasks such as segmentation or tissue classification are known to suffer from domain shifts due to different staining techniques. Stain adaptation aims to reduce the generalization…

Image and Video Processing · Electrical Eng. & Systems 2024-07-04 Daniel Reisenbüchler , Lucas Luttner , Nadine S. Schaadt , Friedrich Feuerhake , Dorit Merhof

Accurate prediction of the likelihood of recurrence is important in the selection of postoperative treatment for patients with early-stage breast cancer. In this study, we investigated whether deep learning algorithms can predict patients'…

Image and Video Processing · Electrical Eng. & Systems 2025-12-22 Geongyu Lee , Joonho Lee , Tae-Yeong Kwak , Sun Woo Kim , Youngmee Kwon , Chungyeul Kim , Hyeyoon Chang

Presenting whole slide images (WSIs) as graph will enable a more efficient and accurate learning framework for cancer diagnosis. Due to the fact that a single WSI consists of billions of pixels and there is a lack of vast annotated datasets…

Image and Video Processing · Electrical Eng. & Systems 2023-06-09 Milan Aryal , Nasim Yahyasoltani

Due to its superior efficiency in utilizing annotations and addressing gigapixel-sized images, multiple instance learning (MIL) has shown great promise as a framework for whole slide image (WSI) classification in digital pathology…

Quantitative Methods · Quantitative Biology 2023-07-14 Qiehe Sun , Jiawen Li , Jin Xu , Junru Cheng , Tian Guan , Yonghong He

Universal, transferable whole-slide image (WSI) representations are central to computational pathology. Incorporating multiple markers (e.g., immunohistochemistry, IHC) alongside H&E enriches H&E-based features with diverse, biologically…

Computer Vision and Pattern Recognition · Computer Science 2025-12-04 Yizhi Zhang , Lei Fan , Zhulin Tao , Donglin Di , Yang Song , Sidong Liu , Cong Cong

While hematoxylin and eosin (H&E) is a standard staining procedure, immunohistochemistry (IHC) staining further serves as a diagnostic and prognostic method. However, acquiring special staining results requires substantial costs. Hence, we…

Computer Vision and Pattern Recognition · Computer Science 2022-08-30 Ming-Yang Ho , Min-Sheng Wu , Che-Ming Wu

Digital whole slide images (WSIs) are generally captured at microscopic resolution and encompass extensive spatial data. Directly feeding these images to deep learning models is computationally intractable due to memory constraints, while…

Image and Video Processing · Electrical Eng. & Systems 2024-11-22 Manahil Raza , Ruqayya Awan , Raja Muhammad Saad Bashir , Talha Qaiser , Nasir M. Rajpoot