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We present a reconstruction technique for simultaneous retrieval of absorption and phase shifting properties of an object recorded by in-line holography. The routine is experimentally tested by applying it to optical holograms of a pure…

Optics · Physics 2010-10-28 Tatiana Latychevskaia , Hans-Werner Fink

The phase unwrapping plays a key role in obtaining a ground-truth phase of the wrapped phase. High-accurate unwrapped phases are demanded in various research fields such as optical holography, optical diffraction tomography, and magnetic…

Optics · Physics 2025-10-08 Alim Yolalmaz , Jeroen Kalkman

In this paper, we propose a new technique for two-dimensional phase unwrapping. The unwrapped phase is found as the solution of an inverse problem that consists in the minimization of an energy functional. The latter includes a weighted…

We address the problem of reconstructing phase-shifting objects from their single shot in-line holograms. We show that a phase-shifting object cannot be reliably recovered from its in-line hologram by non-iterative reconstruction routines,…

Optics · Physics 2015-06-01 Tatiana Latychevskaia , Hans-Werner Fink

We present a dual-wavelength external holographic microscopy module for quantitative phase imaging of 3D structures with extended thickness range. This is done by simultaneous acquisition of two off-axis interferograms, each of which at a…

Optics · Physics 2019-04-10 Nir A. Turko , Natan T. Shaked

We present an image recovery approach to improve amplitude and phase reconstruction from single shot digital holograms, using iterative reconstruction with alternating updates. This approach allows the flexibility to apply different priors…

Optics · Physics 2016-10-04 Dennis J. Lee , Charles A. Bouman , Andrew M. Weiner

Phase recovery from intensity-only measurements forms the heart of coherent imaging techniques and holography. Here we demonstrate that a neural network can learn to perform phase recovery and holographic image reconstruction after…

Computer Vision and Pattern Recognition · Computer Science 2017-12-13 Yair Rivenson , Yibo Zhang , Harun Gunaydin , Da Teng , Aydogan Ozcan

Digital holography is one of the most widely used label-free microscopy techniques in biomedical imaging. Recovery of the missing phase information of a hologram is an important step in holographic image reconstruction. Here we demonstrate…

Image and Video Processing · Electrical Eng. & Systems 2021-05-28 Luzhe Huang , Tairan Liu , Xilin Yang , Yi Luo , Yair Rivenson , Aydogan Ozcan

Recent years have witnessed rapid advancements in 3D scanning technologies, with applications spanning VR/AR, digital human creation, and medical imaging. Structured-light scanning with phase-shifting techniques is preferred for its use of…

Computer Vision and Pattern Recognition · Computer Science 2025-11-25 Xiang Gao , Xinmu Wang , Zhou Zhao , Junqi Huang , Xianfeng David Gu

Here we present practical methods for simulation and reconstruction of in-line digital holograms recorded with plane and spherical waves. The algorithms described here are applicable to holographic imaging of an object exhibiting absorption…

Optics · Physics 2023-06-29 Tatiana Latychevskaia , Hans-Werner Fink

In this paper, we derive a new class of methods for the classic 2D phase unwrapping problem of recovering a phase function from its wrapped form. For this, we consider the wrapped phase as a wavefront aberration in an optical system, and…

Numerical Analysis · Mathematics 2025-03-14 Simon Hubmer , Victoria Laidlaw , Ronny Ramlau , Ekaterina Sherina , Bernadett Stadler

Quantitative phase imaging (QPI) is an emerging label-free technique that produces images containing morphological and dynamical information without contrast agents. Unfortunately, the phase is wrapped in most imaging system. Phase…

Image and Video Processing · Electrical Eng. & Systems 2021-09-01 Fangshu Yang , Thanh-an Pham , Nathalie Brandenberg , Matthias P. Lutolf , Jianwei Ma , Michael Unser

Reconstruction of in-line holograms of unknown objects in general suffers from twin-image artifacts due to the appearance of an out-of-focus image overlapping with the desired image to be reconstructed. Computer-based iterative phase…

Optics · Physics 2021-10-28 Md Sadman Sakib Rahman , Aydogan Ozcan

We present an external interferometric setup that is able to simultaneously acquire three wavelengths of the same sample instance without scanning or multiple exposures. This setup projects onto the monochrome digital camera three off-axis…

We propose an holographic microscopy reconstruction method, which propagates the hologram, in the object half space, in the vicinity of the object. The calibration yields reconstructions with an undistorted reconstruction grid i.e. with…

Optics · Physics 2015-06-16 Nicolas Verrier , Danier Alexandre , Gilles Tessier , Michel Gross

We present a Machine Learning-based method for tomographic reconstruction of dense layered objects, with range of projection angles limited to $\pm $10$^\circ$. Whereas previous approaches to phase tomography generally require two steps,…

Image and Video Processing · Electrical Eng. & Systems 2020-01-08 Alexandre Goy , Girish Rughoobur , Shuai Li , Kwabena Arthur , Akintunde I. Akinwande , George Barbastathis

This paper provides a tutorial of iterative phase retrieval algorithms based on the Gerchberg-Saxton (GS) algorithm applied in digital holography. In addition, a novel GS-based algorithm that allows reconstruction of 3D samples is…

Optics · Physics 2019-11-19 Tatiana Latychevskaia

We introduce a new modality for dynamic phase imaging in confocal microscopy based on synthetic optical holography. By temporal demultiplexing of the detector signal into a series of holograms, we record time-resolved phase images directly…

Optics · Physics 2019-04-09 Martin Schnell , P. Scott Carney , Rainer Hillenbrand

We perform quantitative phase imaging using phase retrieval to implement synthetic aperture imaging. Compared to digital holography, the developed technique is simpler, less expensive, and more stable.

Optics · Physics 2016-09-07 Dennis J. Lee , Andrew M. Weiner

A method is described for the reconstruction of the amplitude and phase of the object exit wave function by phase-plate transmission electron microscopy. The proposed method can be considered as in-line holography and requires three images,…

Materials Science · Physics 2017-05-24 B. Gamm , M. Dries , K. Schultheiss , H. Blank , A. Rosenauer , R. R. Schröder , D. Gerthsen
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