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We present Defocusing Microscopy (DM), a bright-field optical microscopy technique able to perform total 3D imaging of transparent objects. By total 3D imaging we mean the determination of the actual shapes of the upper and lower surfaces…

Biological Physics · Physics 2015-06-19 P. M. S. Roma , L. Siman , F. T. Amaral , U. Agero , O. N. Mesquita

Diffraction tomography is a widely used inverse scattering technique for quantitative imaging of weakly scattering media. In its conventional formulation, diffraction tomography assumes monochromatic plane wave illumination. This…

Numerical Analysis · Mathematics 2026-03-11 Peter Elbau , Noemi Naujoks , Otmar Scherzer

Spatial resolution in optical microscopy has traditionally been treated as a fixed parameter of the optical system. Here, we present an approach to enhance transverse resolution in beam-scanned optical coherence tomography (OCT) beyond its…

Optics · Physics 2021-10-22 Nichaluk Leartprapun , Steven G. Adie

A solution to the inversion problem of scattering would offer aberration-free diffraction-limited 3D images without the resolution and depth-of-field limitations of lens-based tomographic systems. Powerful algorithms are increasingly being…

Optical coherence tomography (OCT) is a non-invasive volumetric imaging modality with high spatial and temporal resolution. For imaging larger tissue structures, OCT probes need to be moved to scan the respective area. For handheld…

Computer Vision and Pattern Recognition · Computer Science 2026-03-24 Suresh Guttikonda , Maximilian Neidhardt , Vidas Raudonis , Alexander Schlaefer

Visible photonic integrated circuits underpin applications ranging from AR/VR to quantum control, yet lack a high-resolution, nondestructive diagnostic comparable to the optical frequency-domain reflectometry used in infrared silicon…

We develop a novel wave imaging scheme for reconstructing the shape of an inhomogeneous scatterer and we consider the inverse acoustic obstacle scattering problem as a prototype model for our study. There exists a wealth of reconstruction…

Analysis of PDEs · Mathematics 2020-01-08 Hongyu Liu , Xiaodong Liu , Xianchao Wang , Yuliang Wang

We consider the inverse problem of reconstructing the scattering coefficient of a simple radiative transport equation (RTE) used to model light propagation inside a scattering medium. To do so, we extract information from the second term in…

Analysis of PDEs · Mathematics 2025-08-07 Francis Chung , Faith Hensley

Optical diffraction tomography measures the three-dimensional refractive index map of a specimen and visualizes biochemical phenomena at the nanoscale in a non-destructive manner. One major drawback of optical diffraction tomography is poor…

Image and Video Processing · Electrical Eng. & Systems 2020-09-30 DongHun Ryu , Dongmin Ryu , YoonSeok Baek , Hyungjoo Cho , Geon Kim , Young Seo Kim , Yongki Lee , Yoosik Kim , Jong Chul Ye , Hyun-Seok Min , YongKeun Park

Optical coherence tomography (OCT) is a volumetric imaging modality that empowers clinicians and scientists to noninvasively visualize the cross-sections of biological samples. As the latest generation of its kind, Fourier-domain OCT…

Image and Video Processing · Electrical Eng. & Systems 2020-05-20 Yuye Ling , Mengyuan Wang , Yu Gan , Xinwen Yao , Leopold Schmetterer , Chuanqing Zhou , Yikai Su

Resolving single atoms in large-scale volumes has been a goal for atomic resolution microscopy for a long time. Electron microscopy has come close to this goal using a combination of advanced electron optics and computational imaging…

Materials Science · Physics 2023-11-07 Andrey Romanov , Min Gee Cho , Mary Cooper Scott , Colin Ophus , Philipp Pelz

X-ray Bragg coherent diffraction imaging has been demonstrated as a powerful three-dimensional (3D) microscopy approach for the investigation of sub-micrometer-scale crystalline particles. It is based on the measurement of a series of…

Total internal reflection fluorescence (TIRF) microscopy and its variants are key technologies for visualizing the dynamics of single molecules or organelles in live cells. Yet, truely quantitative TIRF remains problematic. One unknown…

Biological Physics · Physics 2017-06-07 Maia Brunstein , Lopamudra Roy , Martin Oheim

Visible light optical coherence tomography (vis-OCT) provides a unique tool for imaging the structure and oxygen metabolism in tissues. However, since it works in the spectral domain, vis-OCT still suffers from noises due to the multiple…

Medical Physics · Physics 2021-12-28 Danlei Qiao , Peng Miao , Ian Rubinoff , Jibo Zhou , John B. Troy , Hao F. Zhang , Shanbao Tong

Fiber-form optics extends the high-resolution tomographic imaging capabilities of Optical Coherence Tomography (OCT) to the inside of the human body, i.e., endoscopic OCT. However, it still faces challenges due to the trade-off between…

Optics · Physics 2024-09-04 Chengfu Gu , Haoran Zhang , Qi Lan , Weiyi Zhang , Chang Liu , Jianlong Yang

We present differential phase-contrast optical coherence tomography (DPC-OCT) with two transversally separated probing beams to sense phase gradients in various directions by employing a rotatable Wollaston prism. In combination with a…

Optics · Physics 2011-11-11 Bettina Heise , David Stifter

At the present time Optical Coherence Tomography (OCT) is among the most commonly used non-invasive imaging methods for the acquisition of large volumetric scans of human retinal tissues and vasculature. To resolve decisive information from…

Computer Vision and Pattern Recognition · Computer Science 2021-11-02 D. Sitenko , B. Boll , C. Schnörr

Optoacoustic tomography (OAT), also known as photoacoustic tomography, is an emerging computed biomedical imaging modality that exploits optical contrast and ultrasonic detection principles. Iterative image reconstruction algorithms that…

Medical Physics · Physics 2016-11-17 Kun Wang , Robert W Schoonover , Richard Su , Alexander Oraevsky , Mark A Anastasio

Although the use of multiple stacks can handle slice-to-volume motion correction and artifact removal problems, there are still several problems: 1) The slice-to-volume method usually uses slices as input, which cannot solve the problem of…

Image and Video Processing · Electrical Eng. & Systems 2023-10-17 Junpeng Tan , Xin Zhang , Yao Lv , Xiangmin Xu , Gang Li

Modern 3D reconstruction and novel view synthesis approaches have demonstrated strong performance on scenes with opaque Lambertian objects. However, most assume straight light paths and therefore cannot properly handle refractive and…

Computer Vision and Pattern Recognition · Computer Science 2025-05-19 Yue Yin , Enze Tao , Weijian Deng , Dylan Campbell
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