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Colocalization analyses assess whether two traits are affected by the same or distinct causal genetic variants in a single gene region. A class of Bayesian colocalization tests are now routinely used in practice; for example, for genetic…

Methodology · Statistics 2024-02-20 Ashish Patel , John C. Whittaker , Stephen Burgess

The use of photo-activated fluorescent molecules to create long sequences of low emitter-density diffraction-limited images enables high-precision emitter localization, but at the cost of low temporal resolution. We suggest combining…

Image and Video Processing · Electrical Eng. & Systems 2023-07-19 Gili Dardikman-Yoffe , Yonina C. Eldar

Aiming at improving performance of visual classification in a cost-effective manner, this paper proposes an incremental semi-supervised learning paradigm called Deep Co-Space (DCS). Unlike many conventional semi-supervised learning methods…

Computer Vision and Pattern Recognition · Computer Science 2017-08-01 Ziliang Chen , Keze Wang , Xiao Wang , Pai Peng , Ebroul Izquierdo , Liang Lin

The recent work by Chmyrov et al. (Nature Methods 2013) presents a capstone for the current major super-resolution microscopy techniques. In optical super-resolution microscopy, two pathways are commonly taken: targeted illumination…

Optics · Physics 2015-01-07 Xuanze Chen , Peng Xi

Point-wise localization of individual fluorophores is a critical step in super-resolution microscopy and single particle tracking. Although the methods are limited by the accuracy in localizing individual flourophores, this point-wise…

Biological Physics · Physics 2017-05-08 Martin Lindén , Vladimir Ćurić , Elias Amselem , Johan Elf

Fluorescence Correlation Spectroscopy (FCS) is a powerful single-molecule technique which allows for measuring motion (diffusion, flow), concentration, and molecular interaction kinetics of fluorescent molecules from picomolar to micromolar…

Super-resolution optical fluctuation imaging (SOFI) provides a resolution beyond the diffraction limit by analysing stochastic fluorescence fluctuations with higher-order statistics. Using nth order spatio-temporal cross-cumulants the…

We present a method that can simultaneously locate positions of overlapped multi-emitters at the theoretical-limit precision. We derive a set of simple equations whose solution gives the maximum likelihood estimator of multi-emitter…

Optics · Physics 2015-12-21 Yuto Ashida , Masahito Ueda

Image forensics, aiming to ensure the authenticity of the image, has made great progress in dealing with common image manipulation such as copy-move, splicing, and inpainting in the past decades. However, only a few researchers pay…

Computer Vision and Pattern Recognition · Computer Science 2022-04-26 Yushu Zhang , Nuo Chen , Shuren Qi , Mingfu Xue , Xiaochun Cao

The spatial resolution of images of living samples obtained by fluorescence microscopes is physically limited due to the diffraction of visible light, which makes the study of entities of size less than the diffraction barrier (around 200…

Image and Video Processing · Electrical Eng. & Systems 2023-03-21 Vasiliki Stergiopoulou , Subhadip Mukherjee , Luca Calatroni , Laure Blanc-Féraud

Traditional supervised learning methods have historically encountered certain constraints in medical image segmentation due to the challenging collection process, high labeling cost, low signal-to-noise ratio, and complex features…

Computer Vision and Pattern Recognition · Computer Science 2024-02-20 Yifei Chen , Chenyan Zhang , Yifan Ke , Yiyu Huang , Xuezhou Dai , Feiwei Qin , Yongquan Zhang , Xiaodong Zhang , Changmiao Wang

Single-beam super-resolution microscopy, also known as superlinear microscopy, exploits the nonlinear response of fluorescent probes in confocal microscopy. The technique requires no complex purpose-built system, light field modulation, or…

Multilabel image categorization has drawn interest recently because of its numerous computer vision applications. The proposed work introduces a novel method for classifying multilabel images using the COCO-2014 dataset and a modified…

Computer Vision and Pattern Recognition · Computer Science 2025-11-18 Lokender Singh , Saksham Kumar , Chandan Kumar

High-throughput biological imaging is often constrained by a trade-off between acquisition speed and image quality. Fast imaging modalities, such as wide-field fluorescence microscopy, enable large-scale data acquisition but suffer from…

Image and Video Processing · Electrical Eng. & Systems 2026-04-20 Dominik Panek , Carina Rząca , Maksymilian Szczypior , Joanna Sorysz , Krzysztof Misztal , Zbigniew Baster , Zenon Rajfur

Many microscopy applications are limited by the total amount of usable light and are consequently challenged by the resulting levels of noise in the acquired images. This problem is often addressed via (supervised) deep learning based…

Image and Video Processing · Electrical Eng. & Systems 2020-08-20 Anna S. Goncharova , Alf Honigmann , Florian Jug , Alexander Krull

The high-content image-based assay is commonly leveraged for identifying the phenotypic impact of genetic perturbations in biology field. However, a persistent issue remains unsolved during experiments: the interferential technical noise…

Image and Video Processing · Electrical Eng. & Systems 2022-12-09 Sen Yang , Tao Shen , Yuqi Fang , Xiyue Wang , Jun Zhang , Wei Yang , Junzhou Huang , Xiao Han

The colorectal polyps classification is a critical clinical examination. To improve the classification accuracy, most computer-aided diagnosis algorithms recognize colorectal polyps by adopting Narrow-Band Imaging (NBI). However, the NBI…

Computer Vision and Pattern Recognition · Computer Science 2022-06-27 Weijie Ma , Ye Zhu , Ruimao Zhang , Jie Yang , Yiwen Hu , Zhen Li , Li Xiang

The use of fluorescent molecules to create long sequences of low-density, diffraction-limited images enables highly-precise molecule localization. However, this methodology requires lengthy imaging times, which limits the ability to view…

Image and Video Processing · Electrical Eng. & Systems 2024-03-26 Yair Ben Sahel , Yonina C. Eldar

We establish a method, called pbFFS for photobleaching Fluctuation Fluorescence Spectroscopy, which aims at characterizing molecules or particles labelled with a unknown distribution or fluorophores. Using photobleaching as a control…

A long-standing challenge in multiple-particle-tracking is the accurate and precise 3D localization of individual particles at close proximity. One established approach for snapshot 3D imaging is point-spread-function (PSF) engineering, in…

Image and Video Processing · Electrical Eng. & Systems 2020-10-01 Elias Nehme , Boris Ferdman , Lucien E. Weiss , Tal Naor , Daniel Freedman , Tomer Michaeli , Yoav Shechtman