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Multifocal plane microscopy (MUM) allows three dimensional objects to be imaged in a single camera frame. Our approach uses dual orthogonal diffraction phase gratings with a quadratic distortion of the lines to apply defocus to the first…

Optics · Physics 2020-03-13 Benjamin Gore , Noah Schwartz , Paul Dalgarno

Machine learning is attracting surging interest across nearly all scientific areas by enabling the analysis of large datasets and the extraction of scientific information from incomplete data. Data-driven science is rapidly growing,…

Applied Physics · Physics 2025-03-17 Sung Yun Lee , Do Hyung Cho , Chulho Jung , Daeho Sung , Daewoong Nam , Sangsoo Kim , Changyong Song

Wavefront sensing and reconstruction are widely used for adaptive optics, aberration correction, and high-resolution optical phase imaging. Traditionally, interference and/or microlens arrays are used to convert the optical phase into…

Learning similarity is a key aspect in medical image analysis, particularly in recommendation systems or in uncovering the interpretation of anatomical data in images. Most existing methods learn such similarities in the embedding space…

Computer Vision and Pattern Recognition · Computer Science 2022-06-22 Sukesh Adiga , Jose Dolz , Herve Lombaert

Optical aberrations prevent telescopes from reaching their theoretical diffraction limit. Once estimated, these aberrations can be compensated for using deformable mirrors in a closed loop. Focal plane wavefront sensing enables the…

The most realistic information about the transparent sample such as a live cell can be obtained only using bright-field light microscopy. At high-intensity pulsing LED illumination, we captured a primary 12-bit-per-channel (bpc) response…

Image and Video Processing · Electrical Eng. & Systems 2021-08-18 Ganna Platonova , Dalibor Stys , Pavel Soucek , Kirill Lonhus , Jan Valenta , Renata Rychtarikova

Despite its potential for label-free particle diagnostics, holographic microscopy is limited by specialized processing methods that struggle to generalize across diverse settings. We introduce a deep learning architecture leveraging human…

Optics · Physics 2024-11-26 Shyam Kumar , Jiarong Hong

In the paper we present a low cost optical device which splits the light in the focal plane into two separate optical paths and collimates it back into a single image plane, and where a selective information processing ca be carried out.…

Instrumentation and Methods for Astrophysics · Physics 2016-08-24 Adam Popowicz , Tomasz Blachowicz

Automation in optical microscopy is critical for enabling high-throughput imaging across a wide range of biomedical applications. Among the essential components of automated systems, robust autofocusing plays a pivotal role in maintaining…

Sparse sampling schemes have the potential to dramatically reduce image acquisition time while simultaneously reducing radiation damage to samples. However, for a sparse sampling scheme to be useful it is important that we are able to…

Computer Vision and Pattern Recognition · Computer Science 2017-03-16 G. M. Dilshan P. Godaliyadda , Dong Hye Ye , Michael D. Uchic , Michael A. Groeber , Gregery T. Buzzard , Charles A. Bouman

Recent developments in image acquisition literature have miniaturized the confocal laser endomicroscopes to improve usability and flexibility of the apparatus in actual clinical settings. However, miniaturized devices collect less light and…

Image and Video Processing · Electrical Eng. & Systems 2019-07-24 Saeed Izadi , Darren Sutton , Ghassan Hamarneh

We present a virtual reality display that is capable of generating a dense collection of depth/focal planes. This is achieved by driving a focus-tunable lens to sweep a range of focal lengths at a high frequency and, subsequently, tracking…

Computer Vision and Pattern Recognition · Computer Science 2018-09-25 Jen-Hao Rick Chang , B. V. K. Vijaya Kumar , Aswin C. Sankaranarayanan

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…

The Smart Focal Planes (SmartFP) activity is a European Joint Research Activity funded to develop novel optical technologies for future large telescope instrumentation. In this paper, we will discuss the image slicer developments being…

In scanning microscopy based imaging techniques, there is a need to develop novel data acquisition schemes that can reduce the time for data acquisition and minimize sample exposure to the probing radiation. Sparse sampling schemes are…

Signal Processing · Electrical Eng. & Systems 2018-03-09 Yan Zhang , G. M. Dilshan Godaliyadda , Nicola Ferrier , Emine B. Gulsoy , Charles A. Bouman , Charudatta Phatak

Deep optical optimization has recently emerged as a new paradigm for designing computational imaging systems using only the output image as the objective. However, it has been limited to either simple optical systems consisting of a single…

Computer Vision and Pattern Recognition · Computer Science 2024-08-08 Xinge Yang , Qiang Fu , Wolfgang Heidrich

Accurately tracking particles and determining their coordinate along the optical axis is a major challenge in optical microscopy, especially when extremely high precision is needed. In this study, we introduce a deep learning approach using…

Image and Video Processing · Electrical Eng. & Systems 2025-08-05 Andrey Alexandrov , Giovanni Acampora , Giovanni De Lellis , Antonia Di Crescenzo , Chiara Errico , Daria Morozova , Valeri Tioukov , Autilia Vittiello

To address the demosaicking problem in multispectral polarization filter array (MSPFA) imaging, we propose a multispectral polarization demosaicking network (MSPDNet) that improves image reconstruction accuracy. Imaging with a multispectral…

Image and Video Processing · Electrical Eng. & Systems 2024-10-17 Tomoharu Ishiuchi , Kazuma Shinoda

Deep learning algorithms offer a powerful means to automatically analyze the content of medical images. However, many biological samples of interest are primarily transparent to visible light and contain features that are difficult to…

Computer Vision and Pattern Recognition · Computer Science 2017-09-22 Roarke Horstmeyer , Richard Y. Chen , Barbara Kappes , Benjamin Judkewitz

A shallow depth-of-field image keeps the subject in focus, and the foreground and background contexts blurred. This effect requires much larger lens apertures than those of smartphone cameras. Conventional methods acquire RGB-D images and…

Computer Vision and Pattern Recognition · Computer Science 2022-07-15 Meng-Lin Wu , Venkata Ravi Kiran Dayana , Hau Hwang