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Background fluorescence, especially when it exhibits undesired spatial features, is a primary factor for reduced image quality in optical microscopy. Structured background is particularly detrimental when analyzing single-molecule images…

Optics · Physics 2020-03-25 Leonhard Möckl , Anish R. Roy , Petar N. Petrov , W. E. Moerner

The goal of our research is to develop methods advancing automatic visual recognition. In order to predict the unique or multiple labels associated to an image, we study different kind of Deep Neural Networks architectures and methods for…

Computer Vision and Pattern Recognition · Computer Science 2016-10-19 Rémi Cadène , Nicolas Thome , Matthieu Cord

This paper proposes a robust localization system that employs deep learning for better scene representation, and enhances the accuracy of 6-DOF camera pose estimation. Inspired by the fact that global scene structure can be revealed by wide…

Computer Vision and Pattern Recognition · Computer Science 2019-04-23 Hsin-I Chen , Sebastian Agethen , Chiamin Wu , Winston Hsu , Bing-Yu Chen

Fluorescence microscopy allows for a detailed inspection of cells, cellular networks, and anatomical landmarks by staining with a variety of carefully-selected markers visualized as color channels. Quantitative characterization of…

Computer Vision and Pattern Recognition · Computer Science 2021-08-26 Alvaro Gomariz , Tiziano Portenier , Patrick M. Helbling , Stephan Isringhausen , Ute Suessbier , César Nombela-Arrieta , Orcun Goksel

Software fault localization remains challenging due to limited feature diversity and low precision in traditional methods. This paper proposes a novel approach that integrates multi-objective optimization with deep learning models to…

Software Engineering · Computer Science 2024-11-27 Xiaolei Hu , Dongcheng Li , W. Eric Wong , Ya Zou

Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled…

Biological Physics · Physics 2023-01-25 Jack W Shepherd , Alex L Payne-Dwyer , Ji-Eun Lee , Aisha Syeda , Mark C Leake

A significant research problem of recent interest is the localization of targets like vessels, surgical needles, and tumors in photoacoustic (PA) images. To achieve accurate localization, a high photoacoustic signal-to-noise ratio (SNR) is…

Image and Video Processing · Electrical Eng. & Systems 2021-05-03 Amirsaeed Yazdani , Sumit Agrawal , Kerrick Johnstonbaugh , Sri-Rajasekhar Kothapalli , Vishal Monga

Protein function is inherently linked to its localization within the cell, and fluorescent microscopy data is an indispensable resource for learning representations of proteins. Despite major developments in molecular representation…

Quantitative Methods · Quantitative Biology 2022-05-25 Anastasia Razdaibiedina , Alexander Brechalov

Recently, super-resolution ultrasound imaging with ultrasound localization microscopy (ULM) has received much attention. However, ULM relies on low concentrations of microbubbles in the blood vessels, ultimately resulting in long…

Methods that combine local and global features have recently shown excellent performance on multiple challenging deep image retrieval benchmarks, but their use of local features raises at least two issues. First, these local features simply…

Computer Vision and Pattern Recognition · Computer Science 2022-02-01 Philippe Weinzaepfel , Thomas Lucas , Diane Larlus , Yannis Kalantidis

Error backpropagation is a highly effective mechanism for learning high-quality hierarchical features in deep networks. Updating the features or weights in one layer, however, requires waiting for the propagation of error signals from…

Neural and Evolutionary Computing · Computer Science 2017-11-21 Hesham Mostafa , Vishwajith Ramesh , Gert Cauwenberghs

Single molecule localization microscopy is a recently developed superresolution imaging technique to visualize structural properties of single cells. The basic principle consists in chemically attaching fluorescent dyes to the molecules,…

Object segmentation and structure localization are important steps in automated image analysis pipelines for microscopy images. We present a convolution neural network (CNN) based deep learning architecture for segmentation of objects in…

Computer Vision and Pattern Recognition · Computer Science 2019-01-24 Shan E Ahmed Raza , Linda Cheung , Muhammad Shaban , Simon Graham , David Epstein , Stella Pelengaris , Michael Khan , Nasir M. Rajpoot

Learning-based single image super-resolution (SISR) methods are continuously showing superior effectiveness and efficiency over traditional model-based methods, largely due to the end-to-end training. However, different from model-based…

Image and Video Processing · Electrical Eng. & Systems 2020-03-24 Kai Zhang , Luc Van Gool , Radu Timofte

Three-dimensional (3D) fluorescence microscopy in general requires axial scanning to capture images of a sample at different planes. Here we demonstrate that a deep convolutional neural network can be trained to virtually refocus a 2D…

Computer Vision and Pattern Recognition · Computer Science 2019-11-05 Yichen Wu , Yair Rivenson , Hongda Wang , Yilin Luo , Eyal Ben-David , Laurent A. Bentolila , Christian Pritz , Aydogan Ozcan

Fluorescence microscopy is essential to study biological structures and dynamics. However, existing systems suffer from a tradeoff between field-of-view (FOV), resolution, and complexity, and thus cannot fulfill the emerging need of…

Optics · Physics 2022-09-09 Yujia Xue , Qianwan Yang , Guorong Hu , Kehan Guo , Lei Tian

Counting cells in fluorescent microscopy is a tedious, time-consuming task that researchers have to accomplish to assess the effects of different experimental conditions on biological structures of interest. Although such objects are…

Computer Vision and Pattern Recognition · Computer Science 2021-03-02 R. Morelli , L. Clissa , M. Dalla , M. Luppi , L. Rinaldi , A. Zoccoli

We propose approaches based on deep learning to localize objects in images when only a small training dataset is available and the images have low quality. That applies to many problems in medical image processing, and in particular to the…

Computer Vision and Pattern Recognition · Computer Science 2018-11-20 Aaron Pries , Peter J. Schreier , Artur Lamm , Stefan Pede , Jürgen Schmidt

Traditional deep network training methods optimize a monolithic objective function jointly for all the components. This can lead to various inefficiencies in terms of potential parallelization. Local learning is an approach to…

Machine Learning · Computer Science 2023-01-19 Adeetya Patel , Michael Eickenberg , Eugene Belilovsky

Visual localization is the task of estimating camera pose in a known scene, which is an essential problem in robotics and computer vision. However, long-term visual localization is still a challenge due to the environmental appearance…

Robotics · Computer Science 2022-12-02 Yuxuan Chen , Timothy D. Barfoot