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Digital holographic microscopy is an emerging potentially low-cost alternative to conventional light microscopy for micro-object imaging on earth, underwater and in space. Immediate access to micron-scale objects however requires a…

Optics · Physics 2019-04-10 Stephan Amann , Max von Witzleben , and Stefan Breuer

Fourier ptychographic microscopy (FPM) is a recently developed computational imaging technique for wide-field, high-resolution microscopy with a high space-bandwidth product. It integrates the concepts of synthetic aperture and phase…

Optics · Physics 2021-12-30 Chuanjian Zheng , Shaohui Zhang , Guocheng Zhou , Yao Hu , Qun Hao

Modern imaging techniques at the molecular scale rely on utilizing novel coherent light sources like X-ray free electron lasers for the ultimate goal of visualizing such objects as individual biomolecules rather than crystals. Here, unlike…

Lensless cameras offer significant advantages in size, weight, and cost compared to traditional lens-based systems. Without a focusing lens, lensless cameras rely on computational algorithms to recover the scenes from multiplexed…

Image and Video Processing · Electrical Eng. & Systems 2024-10-08 Xin Cai , Zhiyuan You , Hailong Zhang , Wentao Liu , Jinwei Gu , Tianfan Xue

This paper proposes a non-computational method of counteracting the effect of image degradation introduced by the diffraction phenomenon in lensless microscopy. All the optical images (whether focused by lenses or not) are diffraction…

Optics · Physics 2021-04-08 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab Kumar Dutta

This paper presents a method for estimating the resolution of a digital holographic microscope using neural network analysis of reconstructed images. The spectral bandwidth of the source ($\Delta \lambda$) is used as a controlled image…

Optics · Physics 2026-05-12 A. G. Fedorov

Optical diffraction tomography (ODT) is a three-dimensional (3D) label-free imaging technique. The 3D refractive index distribution of a sample can be reconstructed from multiple two-dimensional optical field images via ODT. Herein, we…

Optics · Physics 2021-02-03 Chansuk Park , Kyeoreh Lee , Yoonseok Baek , Yongkeun Park

Fourier light-field microscopy (FLFM) uses a micro-lens array (MLA) to segment the Fourier Plane of the microscopic objective lens to generate multiple two-dimensional perspective views, thereby reconstructing the three-dimensional(3D)…

Optics · Physics 2022-08-29 Haixin Huang , Haoyuan Qiu , Hanzhe Wu , Yihong Ji , Heng Li , Bin Yu , Danni Chen , Junle Qu

We propose to use high numerical aperture single mode optical fibers like photonic crystal fiber for lensless in-line holographic microscopy. Highly divergent beam helps to overcome the spatial sampling limitation of the image sensor. In…

Optics · Physics 2020-07-01 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab K. Dutta

The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…

Biological Physics · Physics 2024-06-19 Mohamadreza Fazel , Michael J. Wester

We present a lensless, interferenceless incoherent digital holography technique based on the principle of coded aperture correlation holography. The acquired digital hologram by this technique contains a three-dimensional image of some…

Optics · Physics 2017-08-02 Manoj Kumar , A. Vijayakumar , Joseph Rosen

A transverse computer-generated hologram (CGH) diffracts and provides flexible control of incident light by steering it to any point in the projected image plane - i.e. CGHs are able to direct the light to where it is needed and away from…

We report a computational 3D microscopy technique, termed Fourier ptychographic diffraction tomography (FPDT), that iteratively stitches together numerous variably illuminated, low-resolution images acquired with a low-numerical aperture…

Optics · Physics 2020-02-19 Chao Zuo , Jiasong Sun , Jiaji Li , Anand Asundi , Qian Chen

Linear super-resolution microscopy via synthesis aperture approach permits fast acquisition because of its wide-field implementations, however, it has been limited in resolution because a missing spatial-frequency band occurs when trying to…

Ptychography is an enabling coherent diffraction imaging technique for both fundamental and applied sciences. Its applications in optical microscopy, however, fall short for its low imaging throughput and limited resolution. Here, we report…

Dynamic full-field optical coherence microscopy (d-FF-OCM) is a label-free imaging technique that captures intrinsic subcellular motions to generate functional contrast. This dynamic approach yields images with fluorescence-like contrast,…

Optics · Physics 2025-08-06 Erikas Tarvydas , Austeja Treciokaite , Egidijus Auksorius

Holographic microscopy has developed into a powerful tool for 3D particle tracking, yielding nanometer-scale precision at high frame rates. However, current particle tracking algorithms ignore the effect of the microscope objective on the…

Recently, the diffusion model has emerged as a superior generative model that can produce high quality and realistic images. However, for medical image translation, the existing diffusion models are deficient in accurately retaining…

Image and Video Processing · Electrical Eng. & Systems 2023-10-31 Yunxiang Li , Hua-Chieh Shao , Xiao Liang , Liyuan Chen , Ruiqi Li , Steve Jiang , Jing Wang , You Zhang

Fourier ptychographic microscopy (FPM) is a powerful computational imaging modality that achieves high space-bandwidth product imaging for biomedical samples. However, its adoption is limited by slow data acquisition due to the need for…

Optics · Physics 2025-09-09 Shi Zhao , Haowen Zhou , Changhuei Yang

Chapter 1 gives a brief history of the field of holography along with an overview of this thesis. A more detailed description of holography is provided in Chapter 2 along with a discussion of digital holography. Chapter 3 examines the…

Optics · Physics 2009-09-30 John Tapsell