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While high-resolution microscopic techniques are crucial for studying cellular structures in cell biology, obtaining such images from thick 3D engineered tissues remains challenging. In this review, we explore advancements in fluorescence…
The utilization of computational photography becomes increasingly essential in the medical field. Today, imaging techniques for dermatology range from two-dimensional (2D) color imagery with a mobile device to professional clinical imaging…
Histological images are critical in the diagnosis and treatment of cancers. Unfortunately, the current method for capturing these microscopy images require resource intensive tissue preparation that delays diagnosis for many days to a few…
Lightsheet microscopy is a powerful 3-D imaging technique that addresses limitations of traditional optical and confocal microscopy but suffers from a low penetration depth and reduced image quality at greater depths. Multiview lightsheet…
We propose a three-dimensional (3D) multimodal medical imaging system that combines freehand ultrasound and structured light 3D reconstruction in a single coordinate system without requiring registration. To the best of our knowledge, these…
Lensless imaging is an elegant approach to high-resolution microscopy, which is rapidly gaining popularity in applications where imaging optics are problematic. However, current lensless imaging methods require objects to be placed within a…
We present Defocusing Microscopy (DM), a bright-field optical microscopy technique able to perform total 3D imaging of transparent objects. By total 3D imaging we mean the determination of the actual shapes of the upper and lower surfaces…
The possibility to obtain a three-dimensional representation of a single object with sub-$\mu$m resolution is crucial in many fields, from material science to clinical diagnostics. This is typically achieved through tomography, which…
Volumetric biomedical microscopy has the potential to increase the diagnostic information extracted from clinical tissue specimens and improve the diagnostic accuracy of both human pathologists and computational pathology models.…
Biological cell imaging has become one of the most crucial research interests due to its wide-ranging applications in biomedical and microbiology studies. However, three-dimensional (3D) imaging of biological cells remains critically…
We combine confocal imaging, microfluidics and image analysis to record 3D-images of cells in flow. This enables us to recover the full 3D representation of several hundred living cells per minute. Whereas 3D confocal imaging has thus far…
Volumetric biological imaging often involves compromising high temporal resolution at the expense of high spatial resolution when popular scanning methods are used to capture 3D information. We introduce an integrated experimental and image…
Accurate spatial correspondence between medical images is essential for longitudinal analysis, lesion tracking, and image-guided interventions. Medical image registration methods rely on local intensity-based similarity measures, which fail…
A simple method of constructing the 3D surface of non-transparent micro-objects by extending the depth-of-field on the whole attainable surface is presented. The series of images of a sample are recorded by the sequential movement of the…
Matrix imaging paves the way towards a next revolution in wave physics. Based on the response matrix recorded between a set of sensors, it enables an optimized compensation of aberration phenomena and multiple scattering events that usually…
Currently, diagnostic medicine uses a multitude of tools ranging from ionising radiation to histology analysis. With advances in piezoelectric crystal technology, high-frequency ultrasound imaging has developed to achieve comparatively high…
Three-dimensional ultrasound enables real-time volumetric visualization of anatomical structures. Unlike traditional 2D ultrasound, 3D imaging reduces reliance on precise probe orientation, potentially making ultrasound more accessible to…
Objective: The objective of this work is an attempt towards non-contact freehand 3D ultrasound imaging with minimal complexity added to the existing point of care ultrasound (POCUS) systems. Methods: This study proposes a novel approach of…
Structural microvascular alterations and dysfunction serve as key disease indicators of cancer, diabetes, ischemic stroke, neurodegenerative disorders and many other conditions. In vivo visualization of the microvasculature has…
Ultrasound imaging, as a noninvasive, real-time, and low-cost modality, plays a vital role in clinical diagnosis, catheterization intervention, and portable devices. With the development of transducer hardware and the continuous progress of…