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Fluorescence microscopy images usually show severe anisotropy in axial versus lateral resolution. This hampers downstream processing, i.e. the automatic extraction of quantitative biological data. While deconvolution methods and other…

Computer Vision and Pattern Recognition · Computer Science 2017-04-06 Martin Weigert , Loic Royer , Florian Jug , Gene Myers

Image blur and image noise are imaging artifacts intrinsically arising in image acquisition. In this paper, we consider multi-frame blind deconvolution (MFBD), where image blur is described by the convolution of an unobservable,…

Computer Vision and Pattern Recognition · Computer Science 2022-10-04 Leonid Kostrykin , Stefan Harmeling

Three-dimensional (3D) fluorescence microscopy in general requires axial scanning to capture images of a sample at different planes. Here we demonstrate that a deep convolutional neural network can be trained to virtually refocus a 2D…

Computer Vision and Pattern Recognition · Computer Science 2019-11-05 Yichen Wu , Yair Rivenson , Hongda Wang , Yilin Luo , Eyal Ben-David , Laurent A. Bentolila , Christian Pritz , Aydogan Ozcan

In computer-aided diagnosis (CAD) focused on microscopy, denoising improves the quality of image analysis. In general, the accuracy of this process may depend both on the experience of the microscopist and on the equipment sensitivity and…

Image and Video Processing · Electrical Eng. & Systems 2021-05-04 Fabio Hernán Gil Zuluaga , Francesco Bardozzo , Jorge Iván Ríos Patiño , Roberto Tagliaferri

Recent developments in fluorescence microscopy allow capturing high-resolution 3D images over time for living model organisms. To be able to image even large specimens, techniques like multi-view light-sheet imaging record different…

Image and Video Processing · Electrical Eng. & Systems 2021-08-29 Canyu Yang , Dennis Eschweiler , Johannes Stegmaier

Fluorescence microscopy is widely used for the study of biological specimens. Deconvolution can significantly improve the resolution and contrast of images produced using fluorescence microscopy; in particular, Bayesian-based methods have…

Methodology · Statistics 2015-02-04 Alexander Wong , Xiao Yu Wang , Maud Gorbet

The process of acquiring microscopic images in life sciences often results in image degradation and corruption, characterised by the presence of noise and blur, which poses significant challenges in accurately analysing and interpreting the…

Computer Vision and Pattern Recognition · Computer Science 2023-07-18 Tomáš Chobola , Gesine Müller , Veit Dausmann , Anton Theileis , Jan Taucher , Jan Huisken , Tingying Peng

We present a deep learning driven computational approach to overcome the limitations of self-interference digital holography that imposed by inferior axial imaging performances. We demonstrate a 3D deep neural network model can…

In this paper, we propose a fully convolutional networks for iterative non-blind deconvolution We decompose the non-blind deconvolution problem into image denoising and image deconvolution. We train a FCNN to remove noises in the gradient…

Computer Vision and Pattern Recognition · Computer Science 2016-11-22 Jiawei Zhang , Jinshan Pan , Wei-Sheng Lai , Rynson Lau , Ming-Hsuan Yang

Is it possible to recover an image from its noisy version using convolutional neural networks? This is an interesting problem as convolutional layers are generally used as feature detectors for tasks like classification, segmentation and…

Computer Vision and Pattern Recognition · Computer Science 2017-08-02 Nithish Divakar , R. Venkatesh Babu

Image denoising is an important pre-processing step in medical image analysis. Different algorithms have been proposed in past three decades with varying denoising performances. More recently, having outperformed all conventional methods,…

Computer Vision and Pattern Recognition · Computer Science 2017-02-21 Lovedeep Gondara

Deconvolution is the most commonly used image processing method to remove the blur caused by the point-spread-function (PSF) in optical imaging systems. While this method has been successful in deblurring, it suffers from several…

Image and Video Processing · Electrical Eng. & Systems 2019-10-10 Huangxuan Zhao , Ziwen Ke , Ningbo Chen , Ke Li , Lidai Wang , Xiaojing Gong , Wei Zheng , Liang Song , Zhicheng Liu , Dong Liang , Chengbo Liu

Image decomposition is a crucial subject in the field of image processing. It can extract salient features from the source image. We propose a new image decomposition method based on convolutional neural network. This method can be applied…

Computer Vision and Pattern Recognition · Computer Science 2022-08-04 Yu Fu , Xiao-Jun Wu , Josef Kittler

When taking photos in dim-light environments, due to the small amount of light entering, the shot images are usually extremely dark, with a great deal of noise, and the color cannot reflect real-world color. Under this condition, the…

Computer Vision and Pattern Recognition · Computer Science 2019-04-17 Di Zhao , Lan Ma , Songnan Li , Dahai Yu

Volumetric imaging by fluorescence microscopy is often limited by anisotropic spatial resolution from inferior axial resolution compared to the lateral resolution. To address this problem, here we present a deep-learning-enabled…

Computer Vision and Pattern Recognition · Computer Science 2022-07-06 Hyoungjun Park , Myeongsu Na , Bumju Kim , Soohyun Park , Ki Hean Kim , Sunghoe Chang , Jong Chul Ye

In this paper, we propose a novel design of image deblurring in the form of one-shot convolution filtering that can directly convolve with naturally blurred images for restoration. The problem of optical blurring is a common disadvantage to…

Image and Video Processing · Electrical Eng. & Systems 2019-07-22 Mahdi S. Hosseini , Konstantinos N. Plataniotis

Low fluence illumination sources can facilitate clinical transition of photoacoustic imaging because they are rugged, portable, affordable, and safe. However, these sources also decrease image quality due to their low fluence. Here, we…

Image and Video Processing · Electrical Eng. & Systems 2020-04-21 Ali Hariri , Kamran Alipour , Yash Mantri , Jurgen P. Schulze , Jesse V. Jokerst

Fluorescence microscopy is a key driver to promote discoveries of biomedical research. However, with the limitation of microscope hardware and characteristics of the observed samples, the fluorescence microscopy images are susceptible to…

Image and Video Processing · Electrical Eng. & Systems 2022-09-15 Xuanyu Tian , Qing Wu , Hongjiang Wei , Yuyao Zhang

Blind motion deblurring is one of the most basic and challenging problems in image processing and computer vision. It aims to recover a sharp image from its blurred version knowing nothing about the blur process. Many existing methods use…

Computer Vision and Pattern Recognition · Computer Science 2019-01-09 Quan Yuan , Junxia Li , Lingwei Zhang , Zhefu Wu , Guangyu Liu

In multi-photon microscopy (MPM), a recent in-vivo fluorescence microscopy system, the task of image restoration can be decomposed into two interlinked inverse problems: firstly, the characterization of the Point Spread Function (PSF) and…

Image and Video Processing · Electrical Eng. & Systems 2024-11-08 Julien Ajdenbaum , Emilie Chouzenoux , Claire Lefort , Ségolène Martin , Jean-Christophe Pesquet
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