Related papers: Review on methods of solving the refractive index-…
In this chapter, we introduce digital holographic microscopy (DHM) as a marker-free method to determine the refractive index of single, spherical cells in suspension. The refractive index is a conclusive measure in a biological context.…
Intravital microscopy is an essential tool that reveals behaviours of live cells under conditions close to natural physiological states. So far, although various approaches for imaging cells in vivo have been proposed, most require the use…
Holotomography, a three-dimensional quantitative phase imaging technique, presents an innovative, non-invasive approach to studying biological samples by exploiting the refractive index as an intrinsic imaging contrast. Despite offering…
Non-contact measurement of the refractive index and thickness of multilayer biological tissues is of great significance for biomedical applications and can greatly improve medical diagnosis and treatment. In this work, we introduce a…
The detection of spatial or temporal variations in very thin samples has important applications in the biological sciences. For example, cellular membranes exhibit changes in lipid composition and order, which in turn modulate their…
Pixel size in cameras and other refractive imaging devices is typically limited by the free-space diffraction. However, a vast majority of semiconductor-based detectors are based on materials with substantially high refractive index. We…
We suggest a new multimodal imaging technique for quantitatively measuring the integral (thickness-average) refractive index of the nuclei of live biological cells in suspension. For this aim, we combined quantitative phase microscopy with…
A lateral shear interferometer based digital holographic microscopy has been realized to study the morphology dynamics of Human red blood cells quantitatively. Here, a lateral shear interferometer is embedded with a conventional microscope…
Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…
We report extensive enhancement in the refractive index of artificially designed metamaterials by exploiting the deep subwavelength coupling in a free-standing, thin-film metal-dielectric-metal checkboard structure. A record high refractive…
Deconvolution phase microscopy enables high-contrast visualization of transparent samples through reconstructions of their transmitted phases or refractive indexes. Herein, we propose a method to extend 2D deconvolution phase microscopy to…
New lensless diffractive X-ray technic for micro-scale imaging of biological tissue is based on quantitative phase retrieval schemes. By incorporating refraction, this method yields improved contrast compared to purely absorption-based…
We present a cost-effective, simple and fast digital holographic microscopy method based upon Rayleigh-Sommerfeld back propagation for identification of the geometrical shape of a cell. The method was tested using synthetic hologram images…
We developed a microscopy technique that can measure the local refractive index without sampling the optical phase delay of the electromagnetic radiation. To do this, we designed and experimentally demonstrated a setup with two co-localized…
Holographic coherent X-ray imaging enables nanoscale imaging of biological cells and tissues, rendering both phase and absorption contrast, i.e. real and imaginary parts of the refractive index. Unlike the standard model, which assumes a…
We present a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D structured illumination microscopy (SIM).…
Mid-infrared spectroscopy is essential for chemical identification and compositional analysis, due to the existence of characteristic molecular absorption fingerprints. However, it is very challenging to determine the refractive index of an…
Optical diffraction tomography (ODT) is an interferometric microscopy technique capable of measuring 3-D refractive index (RI) distribution of transparent samples. Multiple 2-D holograms of a sample illuminated with various angles are…
We describe here how to apply the three dimensional imaging technique of refrecative index matched scanning to hydrogel spheres. Hydrogels are water based materials with a low refractive index, which allows for index matching with…
White light spectral interferometry is applied to measure the refractive index in absorbing liquids in the spectral range from 400 to 1000 nm. We analyze the influence of absorption on the visibility of interferometric fringes and,…