Related papers: Spectroscopic Approach to Correction and Visualisa…
Investigation of cell structure is hardly imaginable without bright-field microscopy. Numerous modifications such as depth-wise scanning or videoenhancement make this method being state-of-the-art. This raises a question what maximal…
Formation of a bright-field microscopic image of a transparent phase object is described in terms of elementary geometrical optics. Our approach is based on the premise that image replicates the intensity distribution (real or virtual) at…
Light-field microscopy represents a promising solution for microscopic volumetric imaging, thanks to its capability to encode information on multiple planes in a single acquisition. This is achieved through its peculiar simultaneous capture…
Current biological and medical research is aimed at obtaining a detailed spatiotemporal map of a live cell's interior to describe and predict cell's physiological state. We present here an algorithm for complete 3-D modelling of cellular…
It is generally assumed that a high spatial resolution of a microscope requires a large numerical aperture of the imaging lens or detector. In this study, the information limit of 15 pm is achieved in transmission electron microscopy using…
Optical stellar interferometers have demonstrated milli-arcsecond resolution with few apertures spaced hundreds of meters apart. To obtain rich direct images, many apertures will be needed, for a better sampling of the incoming wavefront.…
The mathematical theory of compressed sensing (CS) asserts that one can acquire signals from measurements whose rate is much lower than the total bandwidth. Whereas the CS theory is now well developed, challenges concerning hardware…
Live cell culture is crucial in biomedical studies for analyzing cell properties and dynamics in vitro. This study focuses on segmenting unstained live cells imaged with bright-field microscopy. While many segmentation approaches exist for…
Low-light optical imaging refers to the use of cameras to capture images with minimal photon flux. This area has broad application to diverse fields, including optical microscopy for biological studies. In such studies, it is important to…
Microscopy is the workhorse of the physical and life sciences, producing crisp images of everything from atoms to cells well beyond the capabilities of the human eye. However, the analysis of these images is frequently little better than…
Phase-contrast microscopy converts the optical phase introduced by transparent, unlabeled specimens into modulation in the intensity image. Modern phase imaging techniques are capable of quantifying phase shift at each point in the field of…
In conventional x-ray ptychography, diffraction data is collected by scanning a sample through a monochromatic, and spatially coherent, x-ray beam. A high-resolution image is then retrieved using an iterative algorithm. Combined with a scan…
Recent advances in snapshot multispectral (MS) imaging have enabled compact, low-cost spectral sensors for consumer and mobile devices. By capturing richer spectral information than conventional RGB sensors, these systems can enhance key…
A blind compressive sensing algorithm is proposed to reconstruct hyperspectral images from spectrally-compressed measurements.The wavelength-dependent data are coded and then superposed, mapping the three-dimensional hyperspectral datacube…
Aberration-corrected scanning transmission electron microscopes (STEM) provide sub-angstrom lateral resolution; however, the large convergence angle greatly reduces the depth of field. For microscopes with a small depth of field,…
The expansion of telecommunications incurs increasingly severe crosstalk and interference, and a physical layer cognitive method, called blind source separation (BSS), can effectively address these issues. BSS requires minimal prior…
Information multiplexing is important for biomedical imaging and chemical sensing. In this paper, we report a microscopy imaging technique, termed state-multiplexed Fourier ptychography (FP), for information multiplexing and coherent-state…
We use an empirical approach to characterize the effect of charge-transfer efficiency (CTE) losses in images taken with the Wide-Field Channel of the Advanced Camera for Surveys. The study is based on profiles of warm pixels in 168 dark…
Image simulation remains under-exploited for the most widely used biological phase microscopy methods, because of difficulties in simulating partially coherent illumination. We describe an open-source toolbox, microlith…
Despite its broad availability, volumetric information acquisition from Bright-Field Microscopy (BFM) is inherently difficult due to the projective nature of the acquisition process. We investigate the prediction of 3D cell instances from a…