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Uncovering the heterogeneity of cell populations is a long-standing goal in fields ranging from antimicrobial resistance to cancer research. Emerging technology platforms such as droplet microfluidics hold the promise to decipher cellular…

Image and Video Processing · Electrical Eng. & Systems 2020-01-29 Vasileios Anagnostidis , Benjamin Sherlock , Jeremy Metz , Philip Mair , Florian Hollfelder , Fabrice Gielen

We present a method for segmenting neuron membranes in 2D electron microscopy imagery. This segmentation task has been a bottleneck to reconstruction efforts of the brain's synaptic circuits. One common problem is the misclassification of…

Computer Vision and Pattern Recognition · Computer Science 2020-02-05 Thanuja D. Ambegoda , Matthew Cook

In clinical practice, regions of interest in medical imaging often need to be identified through a process of precise image segmentation. The quality of this image segmentation step critically affects the subsequent clinical assessment of…

Image and Video Processing · Electrical Eng. & Systems 2021-03-23 João B. S. Carvalho , João A. Santinha , Đorđe Miladinović , Joachim M. Buhmann

Convolutional neural networks (CNNs) have achieved state-of-the-art performance for automatic medical image segmentation. However, they have not demonstrated sufficiently accurate and robust results for clinical use. In addition, they are…

Computer Vision and Pattern Recognition · Computer Science 2018-07-23 Guotai Wang , Wenqi Li , Maria A. Zuluaga , Rosalind Pratt , Premal A. Patel , Michael Aertsen , Tom Doel , Anna L. David , Jan Deprest , Sebastien Ourselin , Tom Vercauteren

Fluorescence microscopy has become a widely used tool for studying various biological structures of in vivo tissue or cells. However, quantitative analysis of these biological structures remains a challenge due to their complexity which is…

Computer Vision and Pattern Recognition · Computer Science 2019-04-16 Soonam Lee , Chichen Fu , Paul Salama , Kenneth W. Dunn , Edward J. Delp

Semantic segmentation is the pixel-wise labelling of an image. Since the problem is defined at the pixel level, determining image class labels only is not acceptable, but localising them at the original image pixel resolution is necessary.…

Computer Vision and Pattern Recognition · Computer Science 2022-03-17 Irem Ulku , Erdem Akagunduz

Microscopy imaging techniques are instrumental for characterization and analysis of biological structures. As these techniques typically render 3D visualization of cells by stacking 2D projections, issues such as out-of-plane excitation and…

Image and Video Processing · Electrical Eng. & Systems 2023-02-16 Amirkoushyar Ziabari , Derek C. Rose , Abbas Shirinifard , David Solecki

Like other applications in computer vision, medical image segmentation has been most successfully addressed using deep learning models that rely on the convolution operation as their main building block. Convolutions enjoy important…

Image and Video Processing · Electrical Eng. & Systems 2022-04-05 Davood Karimi , Serge Vasylechko , Ali Gholipour

The ability to extrapolate gene expression dynamics in living single cells requires robust cell segmentation, and one of the challenges is the amorphous or irregularly shaped cell boundaries. To address this issue, we modified the U-Net…

Quantitative Methods · Quantitative Biology 2020-01-17 Nanyan Zhu , Chen Liu , Zakary S. Singer , Tal Danino , Andrew F. Laine , Jia Guo

We propose an automatic preprocessing and ensemble learning for segmentation of cell images with low quality. It is difficult to capture cells with strong light. Therefore, the microscopic images of cells tend to have low image quality but…

Image and Video Processing · Electrical Eng. & Systems 2021-08-31 Sota Kato , Kazuhiro Hotta

Deep learning approaches to 3D shape segmentation are typically formulated as a multi-class labeling problem. Existing models are trained for a fixed set of labels, which greatly limits their flexibility and adaptivity. We opt for top-down…

Computer Vision and Pattern Recognition · Computer Science 2022-01-19 Fenggen Yu , Kun Liu , Yan Zhang , Chenyang Zhu , Kai Xu

We present a novel method for cell segmentation in microscopy images which is inspired by the Generative Adversarial Neural Network (GAN) approach. Our framework is built on a pair of two competitive artificial neural networks, with a…

Computer Vision and Pattern Recognition · Computer Science 2018-09-14 Assaf Arbelle , Tammy Riklin Raviv

We combine confocal imaging, microfluidics and image analysis to record 3D-images of cells in flow. This enables us to recover the full 3D representation of several hundred living cells per minute. Whereas 3D confocal imaging has thus far…

Fluid Dynamics · Physics 2017-09-19 S. Quint , A. F. Christ , A. Guckenberger , S. Himbert , L. Kaestner , S. Gekle , C. Wagner

Semantic segmentation necessitates approaches that learn high-level characteristics while dealing with enormous amounts of data. Convolutional neural networks (CNNs) can learn unique and adaptive features to achieve this aim. However, due…

Computer Vision and Pattern Recognition · Computer Science 2023-07-19 Hasan AlMarzouqi , Lyes Saad Saoud

In this paper, we present contemporary techniques for visualising the feature space of a deep learning image classification neural network. These techniques are viewed in the context of a feed-forward network trained to classify low…

Computer Vision and Pattern Recognition · Computer Science 2018-11-16 Ezra Webb , Cheng Lei , Chun-Jung Huang , Hirofumi Kobayashi , Hideharu Mikami , Keisuke Goda

Live cell microscopy sequences exhibit complex spatial structures and complicated temporal behaviour, making their analysis a challenging task. Considering cell segmentation problem, which plays a significant role in the analysis, the…

Computer Vision and Pattern Recognition · Computer Science 2019-01-08 Assaf Arbelle , Tammy Riklin Raviv

Accurately counting the number of cells in microscopy images is required in many medical diagnosis and biological studies. This task is tedious, time-consuming, and prone to subjective errors. However, designing automatic counting methods…

Image and Video Processing · Electrical Eng. & Systems 2020-11-11 Shenghua He , Kyaw Thu Minn , Lilianna Solnica-Krezel , Mark A. Anastasio , Hua Li

This paper describes a novel method for partitioning image into meaningful segments. The proposed method employs watershed transform, a well-known image segmentation technique. Along with that, it uses various auxiliary schemes such as…

Computer Vision and Pattern Recognition · Computer Science 2013-03-21 Ankit R. Chadha , Neha S. Satam

Accurate localization of organ boundaries is critical in medical imaging for segmentation, registration, surgical planning, and radiotherapy. While deep convolutional networks (ConvNets) have advanced general-purpose edge detection to…

Computer Vision and Pattern Recognition · Computer Science 2026-05-11 Aarav Mehta , Priya Deshmukh , Vikram Singh , Siddharth Malhotra , Krishnan Menon Iyer , Tanvi Iyer

Deep learning based image segmentation methods have achieved great success, even having human-level accuracy in some applications. However, due to the black box nature of deep learning, the best method may fail in some situations. Thus…

Computer Vision and Pattern Recognition · Computer Science 2020-05-28 Leixin Zhou , Wenxiang Deng , Xiaodong Wu
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