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This paper introduces a new method of data-driven microscope design for virtual fluorescence microscopy. Our results show that by including a model of illumination within the first layers of a deep convolutional neural network, it is…

Image and Video Processing · Electrical Eng. & Systems 2020-04-23 Colin L. Cooke , Fanjie Kong , Amey Chaware , Kevin C. Zhou , Kanghyun Kim , Rong Xu , D. Michael Ando , Samuel J. Yang , Pavan Chandra Konda , Roarke Horstmeyer

The study of neurodegenerative diseases relies on the reconstruction and analysis of the brain cortex from magnetic resonance imaging (MRI). Traditional frameworks for this task like FreeSurfer demand lengthy runtimes, while its accelerated…

Image and Video Processing · Electrical Eng. & Systems 2020-10-23 Rodrigo Santa Cruz , Leo Lebrat , Pierrick Bourgeat , Clinton Fookes , Jurgen Fripp , Olivier Salvado

How microorganisms respond to and interact with their environment can vary significantly from individual to individual, which can have important microbiological and ecological implications. However, most microscopy techniques can only…

Deep neural networks have been applied to improve the image quality of fluorescence microscopy imaging. Previous methods are based on convolutional neural networks (CNNs) which generally require more time-consuming training of separate…

Fluoroscopy is critical for real-time X-ray visualization in medical imaging. However, low-dose images are compromised by noise, potentially affecting diagnostic accuracy. Noise reduction is crucial for maintaining image quality, especially…

Image and Video Processing · Electrical Eng. & Systems 2024-11-05 Sun-Young Jeon , Sen Wang , Adam S. Wang , Garry E. Gold , Jang-Hwan Choi

Volumetric biological imaging often involves compromising high temporal resolution at the expense of high spatial resolution when popular scanning methods are used to capture 3D information. We introduce an integrated experimental and image…

The detection of cell shape changes in 3D time-lapse images of complex tissues is an important task. However, it is a challenging and tedious task to establish a comprehensive dataset to improve the performance of deep learning models. In…

Image and Video Processing · Electrical Eng. & Systems 2019-06-04 Dali Wang , Zheng Lu , Zhirong Bao

Object segmentation and structure localization are important steps in automated image analysis pipelines for microscopy images. We present a convolution neural network (CNN) based deep learning architecture for segmentation of objects in…

Computer Vision and Pattern Recognition · Computer Science 2019-01-24 Shan E Ahmed Raza , Linda Cheung , Muhammad Shaban , Simon Graham , David Epstein , Stella Pelengaris , Michael Khan , Nasir M. Rajpoot

Fluorescence microscopy has been a significant tool to observe long-term imaging of embryos (in vivo) growth over time. However, cumulative exposure is phototoxic to such sensitive live samples. While techniques like light-sheet…

Image and Video Processing · Electrical Eng. & Systems 2022-01-05 Varun Mannam , Jacob Brandt , Cody J. Smith , Scott Howard

In this paper, we adopt 3D Convolutional Neural Networks to segment volumetric medical images. Although deep neural networks have been proven to be very effective on many 2D vision tasks, it is still challenging to apply them to 3D tasks…

Computer Vision and Pattern Recognition · Computer Science 2018-08-03 Zhuotun Zhu , Yingda Xia , Wei Shen , Elliot K. Fishman , Alan L. Yuille

One of the most common tasks in medical imaging is semantic segmentation. Achieving this segmentation automatically has been an active area of research, but the task has been proven very challenging due to the large variation of anatomy…

Computer Vision and Pattern Recognition · Computer Science 2018-04-10 Holger R. Roth , Chen Shen , Hirohisa Oda , Masahiro Oda , Yuichiro Hayashi , Kazunari Misawa , Kensaku Mori

As many different 3D volumes could produce the same 2D x-ray image, inverting this process is challenging. We show that recent deep learning-based convolutional neural networks can solve this task. As the main challenge in learning is the…

Graphics · Computer Science 2018-11-29 Philipp Henzler , Volker Rasche , Timo Ropinski , Tobias Ritschel

One primary technical challenge in photoacoustic microscopy (PAM) is the necessary compromise between spatial resolution and imaging speed. In this study, we propose a novel application of deep learning principles to reconstruct…

Image and Video Processing · Electrical Eng. & Systems 2020-06-02 Anthony DiSpirito , Daiwei Li , Tri Vu , Maomao Chen , Dong Zhang , Jianwen Luo , Roarke Horstmeyer , Junjie Yao

Light-field microscopes are able to capture spatial and angular information of incident light rays. This allows reconstructing 3D locations of neurons from a single snap-shot.In this work, we propose a model-inspired deep learning approach…

Image and Video Processing · Electrical Eng. & Systems 2021-03-11 Pingfan Song , Herman Verinaz Jadan , Carmel L. Howe , Peter Quicke , Amanda J. Foust , Pier Luigi Dragotti

Today the gold standard for in vivo imaging through scattering tissue is the point-scanning two-photon microscope (PSTPM). Especially in neuroscience, PSTPM is widely used for deep-tissue imaging in the brain. However, due to sequential…

Image and Video Processing · Electrical Eng. & Systems 2020-01-03 Zhun Wei , Josiah R. Boivin , Yi Xue , Xudong Chen , Peter T. C. So , Elly Nedivi , Dushan N. Wadduwage

Recent developments in fluorescence microscopy allow capturing high-resolution 3D images over time for living model organisms. To be able to image even large specimens, techniques like multi-view light-sheet imaging record different…

Image and Video Processing · Electrical Eng. & Systems 2021-08-29 Canyu Yang , Dennis Eschweiler , Johannes Stegmaier

Holography encodes the three dimensional (3D) information of a sample in the form of an intensity-only recording. However, to decode the original sample image from its hologram(s), auto-focusing and phase-recovery are needed, which are in…

Computer Vision and Pattern Recognition · Computer Science 2018-12-31 Yichen Wu , Yair Rivenson , Yibo Zhang , Zhensong Wei , Harun Gunaydin , Xing Lin , Aydogan Ozcan

Computational Cannula Microscopy (CCM) is a high-resolution widefield fluorescence imaging approach deep inside tissue, which is minimally invasive. Rather than using conventional lenses, a surgical cannula acts as a lightpipe for both…

Image and Video Processing · Electrical Eng. & Systems 2020-12-02 Ruipeng Guo , Zhimeng Pan , Andrew Taibi , Jason Shepherd , Rajesh Menon

Medical images, especially volumetric images, are of high resolution and often exceed the capacity of standard desktop GPUs. As a result, most deep learning-based medical image analysis tasks require the input images to be downsampled,…

Image and Video Processing · Electrical Eng. & Systems 2021-08-29 Jianning Li , Antonio Pepe , Christina Gsaxner , Yuan Jin , Jan Egger

Differentiable simulations of optical systems can be combined with deep learning-based reconstruction networks to enable high performance computational imaging via end-to-end (E2E) optimization of both the optical encoder and the deep…

Image and Video Processing · Electrical Eng. & Systems 2022-11-04 Diptodip Deb , Zhenfei Jiao , Ruth Sims , Alex B. Chen , Michael Broxton , Misha B. Ahrens , Kaspar Podgorski , Srinivas C. Turaga