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Fluorescence microscopy plays an important role in biomedical research. The depth-variant point spread function (PSF) of a fluorescence microscope produces low-quality images especially in the out-of-focus regions of thick specimens.…

Image and Video Processing · Electrical Eng. & Systems 2019-07-09 Da He , De Cai , Jiasheng Zhou , Jiajia Luo , Sung-Liang Chen

We have developed a novel method for co-adding multiple under-sampled images that combines the iteratively reweighted least squares and divide-and-conquer algorithms. Our approach not only allows for the anti-aliasing of the images but also…

Instrumentation and Methods for Astrophysics · Physics 2024-02-29 Lei Wang , Huanyuan Shan , Lin Nie , Dezi Liu , Zhaojun Yan , Guoliang Li , Cheng Cheng , Yushan Xie , Han Qu , Wenwen Zheng , Xi Kang

We propose a new method to create compact convolutional neural networks (CNNs) by exploiting sparse convolutions. Different from previous works that learn sparsity in models, we directly employ hand-crafted kernels with regular sparse…

Computer Vision and Pattern Recognition · Computer Science 2018-09-12 Chun-Fu Chen , Quanfu Fan , Marco Pistoia , Gwo Giun Lee

Despeckling is a crucial noise reduction task in improving the quality of synthetic aperture radar (SAR) images. Directly obtaining noise-free SAR images is a challenging task that has hindered the development of accurate despeckling…

Computer Vision and Pattern Recognition · Computer Science 2024-01-17 Shunya Kato , Masaki Saito , Katsuhiko Ishiguro , Sol Cummings

We report a new coherent imaging technique, termed ptychographic structured modulation (PSM), for quantitative super-resolution microscopy. In this technique, we place a thin diffuser (i.e., a scattering lens) in between the sample and the…

Image and Video Processing · Electrical Eng. & Systems 2019-07-26 Pengming Song , Shaowei Jiang , He Zhang , Zichao Bian , Chengfei Guo , Kazunori Hoshino , Guoan Zheng

Fluorescence microscopy is essential in biological and medical research, providing critical insights into cellular structures. However, limited by optical diffraction and background noise, a substantial amount of hidden information is still…

Biological Physics · Physics 2026-04-14 Xiaofeng Zhang , Yongsheng Huang , Jielong Yang , Zhili Wang , Si Chen , Linbo Liu , Xin Ge

Single Molecule Localization Microscopy (SMLM) enables the acquisition of high-resolution images by alternating between activation of a sparse subset of fluorescent molecules present in a sample and localization. In this work, the…

Image and Video Processing · Electrical Eng. & Systems 2018-12-17 Arne Bechensteen , Laure Blanc-Féraud , Gilles Aubert

We present high resolution two-photon excitation microscopy studies combining two-photon fluorescence (TPF) and second harmonic generation (SHG) in order to probe orientational distributions of molecular ensembles at room temperature. A…

Soft Condensed Matter · Physics 2007-05-23 Veronique Le Floc'h , Sophie Brasselet , Jean-Francois Roch , Joseph Zyss

Accurate blur estimation is essential for high-performance imaging across various applications. Blur is typically represented by the point spread function (PSF). In this paper, we propose a physics-informed PSF learning framework for…

Computer Vision and Pattern Recognition · Computer Science 2025-03-12 Liqun Chen , Yuxuan Li , Jun Dai , Jinwei Gu , Tianfan Xue

We propose an autofocusing algorithm to obtain, relatively accurately, the 3D position of each particle, particularly its axial location, and particle number of a dense transparent particle solution via its hologram. First, morphological…

Signal Processing · Electrical Eng. & Systems 2025-03-25 Wei-Na Li , Yi Zhou , Jiatai Chen , Hongjie Ou , XiangSheng Xie

Single-beam super-resolution microscopy, also known as superlinear microscopy, exploits the nonlinear response of fluorescent probes in confocal microscopy. The technique requires no complex purpose-built system, light field modulation, or…

We propose an image deconvolution algorithm when the data is contaminated by Poisson noise. The image to restore is assumed to be sparsely represented in a dictionary of waveforms such as the wavelet or curvelet transforms. Our key…

Applications · Statistics 2009-11-13 François-Xavier Dupé , Jalal Fadili , Jean Luc Starck

Access to knowledge of the point spread function (PSF) of adaptive optics(AO)-assisted observations is still a major limitation when processing AO data. This limitation is particularly important when image analysis requires the use of…

Instrumentation and Methods for Astrophysics · Physics 2023-05-10 A. Lau , R. JL. Fétick , B. Neichel , O. Beltramo-Martin , T. Fusco

Confocal microscopy in combination with real-space particle tracking has proven to be a powerful tool in scientific fields such as soft matter physics, materials science and cell biology. However, 3D tracking of anisotropic particles in…

Soft Condensed Matter · Physics 2015-05-20 T. H. Besseling , M. Hermes , A. Kuijk , B. de Nijs , T. -S. Deng , M. Dijkstra , A. Imhof , A. van Blaaderen

Restoring images degraded by spatially varying blur is a problem encountered in many disciplines such as astrophysics, computer vision or biomedical imaging. One of the main challenges to perform this task is to design efficient numerical…

Optimization and Control · Mathematics 2015-10-13 Paul Escande , Pierre Weiss

We propose a network for semantic mapping called the Dense Dilated Convolutions Merging Network (DDCM-Net) to provide a deep learning approach that can recognize multi-scale and complex shaped objects with similar color and textures, such…

Computer Vision and Pattern Recognition · Computer Science 2019-09-02 Qinghui Liu , Michael Kampffmeyer , Robert Jenssen , Arnt-Børre Salberg

In this paper, we present a novel deep learning architecture for infrared and visible images fusion problem. In contrast to conventional convolutional networks, our encoding network is combined by convolutional layers, fusion layer and…

Computer Vision and Pattern Recognition · Computer Science 2019-01-23 Hui Li , Xiao-Jun Wu

Magnetic Resonance Imaging (MRI) offers high-resolution \emph{in vivo} imaging and rich functional and anatomical multimodality tissue contrast. In practice, however, there are challenges associated with considerations of scanning costs,…

Computer Vision and Pattern Recognition · Computer Science 2017-05-09 Yawen Huang , Ling Shao , Alejandro F. Frangi

Image segmentation is widely used in a variety of computer vision tasks, such as object localization and recognition, boundary detection, and medical imaging. This thesis proposes deep learning architectures to improve automatic object…

Image and Video Processing · Electrical Eng. & Systems 2019-09-24 Debleena Sengupta

This paper addresses the deconvolution of an image that has been obtained by superimposing many copies of an underlying unknown image of interest. The superposition is assumed to not be exact due to noise, and is described using an error…

Numerical Analysis · Mathematics 2007-10-04 Wooram Park , Daniel N. Rockmore , Dean Madden , Gregory S. Chirikjian