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Fourier ptychographic microscopy (FPM) is a promising quantitative phase imaging technique that enables high-resolution, label-free imaging over a large field-of-view. Here, we present the first application of FPM for the quantitative…

Fourier ptychographic microscopy (FPM) is a recently developed computational imaging technique for wide-field, high-resolution microscopy with a high space-bandwidth product. It integrates the concepts of synthetic aperture and phase…

Optics · Physics 2021-12-30 Chuanjian Zheng , Shaohui Zhang , Guocheng Zhou , Yao Hu , Qun Hao

Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the universal…

Multiple-input multiple-output (MIMO) orthogonal frequency-division multiplexing (OFDM) systems rely on digital or hybrid digital and analog designs for beamforming against frequency-selective fading, which suffer from high hardware…

Signal Processing · Electrical Eng. & Systems 2026-01-26 Zheao Li , Jiancheng An , Chau Yuen

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…

We report a novel microscopy platform, termed Multi-Aperture Fourier ptychographic microscopy (MA-FPM), capable of realizing gigapixel complex field images with large data acquisition bandwidths - in gigapixels per second. MA-FPM is a…

Optics · Physics 2020-10-14 Pavan Chandra Konda , Jonathan M. Taylor , Andrew R. Harvey

Photoacoustic microscopy is becoming an important tool for the biomedical research. It has been widely used in biological researches, such as structural imaging of vasculature, brain structural and functional imaging, and tumor detection.…

Image and Video Processing · Electrical Eng. & Systems 2020-09-09 Xianlin Song , Jianshuang Wei , Lingfang Song

For quantitative phase imaging (QPI) based on transport-of-intensity equation (TIE), partially coherent illumination provides speckle-free imaging, compatibility with brightfield microscopy, and transverse resolution beyond coherent…

Optics · Physics 2017-05-31 Chao Zuo , Jiasong Sun , Jiaji Li , Jialin Zhang , Anand Asundi , Qian Chen

We have developed an automatic method for segmenting fluorescence lifetime (FLT) imaging microscopy (FLIM) images of cells inspired by a multi-resolution community detection (MCD) based network segmentation method. The image processing…

Medical Physics · Physics 2014-01-06 Dandan Hu , Pinaki Sarder , Peter Ronhovde , Sandra Orthaus , Samuel Achilefu , Zohar Nussinov

Fourier ptychography microscopy (FPM) is a new computational imaging technique that can provide gigapixel images with both high resolution and a wide field of view (FOV). However, time consuming of the data-acquisition process is a critical…

Image and Video Processing · Electrical Eng. & Systems 2018-08-15 Ao Zhou , Ni Chen , Haichao Wang , Guohai Situ

Spectral imaging enables spatially-resolved identification of materials in remote sensing, biomedicine, and astronomy. However, acquisition times require balancing spectral and spatial resolution with signal-to-noise. Hyperspectral imaging…

Computer Vision and Pattern Recognition · Computer Science 2020-03-17 Nguyen Tran , Rupali Mankar , David Mayerich , Zhu Han

Structured illumination enables the tailoring of an imaging device's optical transfer function to enhance resolution. We propose the incorporation of a temporal periodic modulation, specifically a rotating mask, to encode multiple transfer…

Optics · Physics 2023-06-27 Guillaume Noetinger , Fabrice Lemoult , Sébastien M. Popoff

Light-sheet microscopes must compromise between field of view, optical sectioning, resolution, and detection efficiency. High-numerical-aperture (NA) detection objective lenses provide high resolution but their narrow depth of field fails…

We present a simple and compact approach of structured illumination microscopy by using three $2\times2$ fiber couplers and one $1\times4$ MEMS optics switch. One uniform and three fringe illumination patterns were produced by placing seven…

Optics · Physics 2019-12-18 Shiming Hu , Wenwen Liu , Junyao Jie , Yizheng Huang , Qingquan Wei , Manqing Tan , Yude Yu

Confocal laser scanning microscopy (CLSM) stands out as one of the most widely used microscopy techniques, thanks to its three-dimensional imaging capability and its sub-diffraction spatial resolution, achieved through the closure of a…

Fourier ptychographic microscopy (FPM) is a recently proposed computational imaging technique with both high resolution and wide field-of-view. In current FP experimental setup, the dark-field images with high-angle illuminations are easily…

Computer Vision and Pattern Recognition · Computer Science 2017-12-19 Yan Zhang , An Pan , Ming Lei , Baoli Yao

Imaging beyond the diffraction limit barrier has attracted wide attention due to the ability to resolve image features that were previously hidden. Of the various super-resolution microscopy techniques available, a particularly simple…

Stacked intelligent metasurfaces (SIM) is a revolutionary technology, which can outperform its single-layer counterparts by performing advanced signal processing relying on wave propagation. In this work, we exploit SIM to enable transmit…

Fourier ptychography microscopy (FPM), sharing its roots with synthetic aperture technique and phase retrieval method, is a recently developed computational microscopic super-resolution technique. By turning on the light-emitting diode…

Optics · Physics 2021-05-07 G. Zhou , S. Zhang , C. Zheng , T. Li , Y. Hu , Q. Hao

Lensless on-chip microscopy has shown great potential for biomedical imaging due to its large-area and high-throughput imaging capabilities. By combining the pixel super-resolution (PSR) technique, it can improve the resolution beyond the…

Image and Video Processing · Electrical Eng. & Systems 2024-09-06 Xuyang Chang , Shaowei Jiang , Yongcun Hu , Liheng Bian