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The development of multicellular organisms relies on the precise coordination of molecular events across multiple spatial and temporal scales. Understanding how information flows from molecular interactions to cellular processes and tissue…

Biological Physics · Physics 2025-01-29 Andrés H. Cardona , Márcia Mesquita Peixoto , Tohn Borjigin , Thomas Gregor

We propose a general theory to describe the distribution of protein-folding transition paths. We show that transition paths follow a predictable sequence of high-free-energy transient states that are separated by free-energy barriers. Each…

Biomolecules · Quantitative Biology 2016-09-21 William M. Jacobs , Eugene I. Shakhnovich

The classic model of eukaryotic gene expression requires direct spatial contact between a distal enhancer and a proximal promoter. Recent Chromosome Conformation Capture (3C) studies show that enhancers and promoters are embedded in a…

Genomics · Quantitative Biology 2014-11-25 Boryana Doyle , Geoffrey Fudenberg , Maxim Imakaev , Leonid A. Mirny

We propose a model for the formation of chromatin loops based on the diffusive sliding of a DNA-bound factor which can dimerise to form a molecular slip-link. Our slip-links mimic the behaviour of cohesin-like molecules, which, along with…

Biological Physics · Physics 2017-10-04 C. A. Brackley , J. Johnson , D. Michieletto , A. N. Morozov , M. Nicodemi , P. R. Cook , D. Marenduzzo

Characterizing the link between small-scale chromatin structure and large-scale chromosome folding during interphase is a prerequisite for understanding transcription. Yet, this link remains poorly investigated. Here, we introduce a simple…

Soft Condensed Matter · Physics 2016-09-28 Ana Maria Florescu , Pierre Therizols , Angelo Rosa

This paper focuses on mechanical aspects of chromatin biological functioning. Within a basic geometric modeling of the chromatin assembly, we give for the first time the complete set of elastic constants (twist and bend persistence lengths,…

Soft Condensed Matter · Physics 2016-08-16 Eli Ben-Haïm , Annick Lesne , Jean-Marc Victor

Polymers with active segments constitute prospective future materials and are used as a model for some biological systems such as chromatin. The directions of the active forces are typically introduced with temporal or spatial correlations…

Soft Condensed Matter · Physics 2024-11-25 Adam H. T. P. Höfler , Iurii Chubak , Christos N. Likos , Jan Smrek

Recent progress has been made in the understanding of the physical properties of chromatin -- the dense complex of DNA and histone proteins that occupies the nuclei of plant and animal cells. Here I will focus on the two lowest levels of…

Soft Condensed Matter · Physics 2007-05-23 Helmut Schiessel

The chromatin fiber is a complex of DNA and specific proteins called histones forming the first structural level of organization of eukaryotic chromosomes. In tightly organized chromatin fibers, the short segments of naked DNA linking the…

Statistical Mechanics · Physics 2016-08-16 Jean-Marc Victor , Eli Ben-Haïm , Annick Lesne

Chromosomes are crumpled polymer chains further folded into a sequence of stochastic loops via loop extrusion. While extrusion has been verified experimentally, the particular means by which the extruding complexes bind DNA polymer remains…

Soft Condensed Matter · Physics 2023-06-14 Kirill Polovnikov , Bogdan Slavov

We propose a polymer model for the dynamics of chromatin replication in three dimensional space. Our simulations indicate that both immobile and tracking replisomes may self-assemble during the process, reconciling previous apparently…

Biological Physics · Physics 2023-12-19 G. Forte , S. Buonomo , P. R. Cook , N. Gilbert , D. Marenduzzo , E. Orlandini

The phase behavior of charged rods in the presence of inter-rod linkers is studied theoretically as a model for the equilibrium behavior underlying the organization of actin filaments by linker proteins in the cytoskeleton. The presence of…

Soft Condensed Matter · Physics 2016-08-31 Itamar Borukhov , Robijn F. Bruinsma , William M. Gelbart , Andrea J. Liu

Recent experiments have been able to visualise chromosome organization in fast-growing E.coli cells. However, the mechanism underlying the spatio-temporal organization remains poorly understood. We propose that the DNA adopts a specific…

Soft Condensed Matter · Physics 2026-04-06 Shreerang Pande , Debarshi Mitra , Apratim Chatterji

The motion involved in barrier crossing for protein folding are investigated in terms of the chain dynamics of the polymer backbone, completing the microscopic description of protein folding presented in the previous paper. Local reaction…

Soft Condensed Matter · Physics 2009-10-31 John J. Portman , Shoji Takada , Peter G. Wolynes

While the structure of chromatin has been studied in great detail on length scales below 30 nm, amazingly little is known about the higher-order folding motifs of chromatin in interphase. Recent experiments give evidence that the folding…

Soft Condensed Matter · Physics 2009-02-16 Manfred Bohn , Dieter W. Heermann , Roel van Driel

Spatiotemporal coordination of chromatin and subnuclear compartments is crucial for cells. Numerous enzymes act inside nucleus\textemdash some of those transiently link and pass two chromatin segments. Here we study how such an active…

Biological Physics · Physics 2024-01-18 Rakesh Das , Takahiro Sakaue , G. V. Shivashankar , Jacques Prost , Tetsuya Hiraiwa

During interphase, a typical cell nucleus features spatial compartmentalization of transcriptionally active euchromatin and repressed heterochromatin domains. In conventional nuclear organization, euchromatin predominantly occupies the…

Soft Condensed Matter · Physics 2025-07-25 Ligesh Theeyancheri , Edward J. Banigan , J. M. Schwarz

Transcription is a fundamental cellular process, and the first step of gene expression. In human cells, it depends on the binding to chromatin of various proteins, including RNA polymerases and numerous transcription factors (TFs).…

Monte Carlo simulations of coarse-grained polymers provide a useful tool to deepen the understanding of conformational and statistical properties of polymers both in physical as well as in biological systems. In this study we sample compact…

Soft Condensed Matter · Physics 2009-05-07 Manfred Bohn , Dieter W. Heermann

Mechanically induced folding of passive cross-linkers is a fundamental biological phenomenon. A typical example is a conformational change in myosin II responsible for the power-stroke in skeletal muscles. In this paper we present an…

Biological Physics · Physics 2017-09-14 Matthieu Caruel , Jean-Marc Allain , Lev Truskinovsky