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Optical spectrometers are widely used scientific equipment with many applications involving material characterization, chemical analysis, disease diagnostics, surveillance, etc. Emerging applications in biomedical and communication fields…

Structured illumination microscopy (SIM) has emerged as an essential technique for 3D and live-cell super-resolution imaging. However, to date, there has not been a dedicated workshop or journal issue covering the various aspects of SIM,…

Among super-resolution microscopy techniques, structured illumination microscopy (SIM) shows great advances of low phototoxicity, high speed, and excellent performance in long-term dynamic observation, making it especially suitable for live…

Optics · Physics 2025-02-07 Han Wang , Wenshu Wang , Xinzhu Xu , Meiqi Li , Peng Xi

We present a simple and compact approach of structured illumination microscopy by using three $2\times2$ fiber couplers and one $1\times4$ MEMS optics switch. One uniform and three fringe illumination patterns were produced by placing seven…

Optics · Physics 2019-12-18 Shiming Hu , Wenwen Liu , Junyao Jie , Yizheng Huang , Qingquan Wei , Manqing Tan , Yude Yu

Structured illumination microscopy (SIM) can achieve a $2\times$ resolution enhancement beyond the classical diffraction limit by employing illumination translations with respect to the object. This method has also been successfully…

In this paper, a compact and low-cost structured illumination microscope (SIM) based on a 2X2 fiber coupler is presented. Fringe illumination is achieved by placing two output fiber tips at a conjugate Fourier plane of the sample plane as…

Instrumentation and Detectors · Physics 2018-12-19 Shiming Hu , Lei Liu , Yizheng Huang , Wenwen Liu , Qingquan Wei , Manqing Tan , Yude Yu

High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy. Here, we use…

Image and Video Processing · Electrical Eng. & Systems 2019-03-28 Li-Hao Yeh , Shwetadwip Chowdhury , Laura Waller

Over the past decade, structured illumination microscopy (SIM) has found its niche in super-resolution (SR) microscopy due to its fast imaging speed and low excitation intensity. However, due to the significantly higher light dose compared…

We present a novel method to reconstruct a spectral central view and its aligned disparity map from spatio-spectrally coded light fields. Since we do not reconstruct an intermediate full light field from the coded measurement, we refer to…

Computer Vision and Pattern Recognition · Computer Science 2021-10-15 Maximilian Schambach , Jiayang Shi , Michael Heizmann

Super-resolution structured illumination microscopy (SR-SIM) is a widely used technique for enhancing the resolution of fluorescence imaging beyond the diffraction limit. Most existing SR-SIM methods rely on Moir\'e effect-based physical…

Optics · Physics 2025-02-27 Junzheng Peng , Jiahao Xian , Xi Lin , Manhong Yao , Shiping Li , Jingang Zhong

The wavefront is scrambled when coherent light propagates through a random scattering medium and which makes direct use of the conventional optical methods ineffective. In this paper, we propose and demonstrate a structured light…

We describe a "spatio-spectral" deconvolution algorithm for wide-band imaging in radio interferometry. In contrast with the existing multi-frequency reconstruction algorithms, the proposed method does not rely on a model of the…

Instrumentation and Methods for Astrophysics · Physics 2016-03-01 André Ferrari , Jérémy Deguignet , Chiara Ferrari , David Mary , Antony Schutz , Oleg Smirnov

A unified method for three-dimensional reconstruction of objects from transmission images collected at multiple illumination directions is described. The method may be applicable to experimental conditions relevant to absorption-based,…

Medical Physics · Physics 2022-12-07 Timur E. Gureyev , Hamish G. Brown , Harry M. Quiney , Leslie J. Allen

Structured illumination microscopy (SIM) achieves superresolution in fluorescence imaging through patterned illumination and computational image reconstruction, yet current methods require bulky, costly modulation optics and high-precision…

Optics · Physics 2024-04-15 Tamal Roy , Peter T. Brown , Douglas P. Shepherd , Lisa V. Poulikakos

A new spectral conjugate subgradient method is presented to solve nonsmooth unconstrained optimization problems. The method combines the spectral conjugate gradient method for smooth problems with the spectral subgradient method for…

Optimization and Control · Mathematics 2025-10-10 Milagros Loreto , Thomas Humphries , Chella Raghavan , Kenneth Wu , Sam Kwak

An axiomatic approach to signal reconstruction is formulated, involving a sample consistent set and a guiding set, describing desired reconstructions. New frame-less reconstruction methods are proposed, based on a novel concept of a…

Information Theory · Computer Science 2017-02-14 Andrew Knyazev , Akshay Gadde , Hassan Mansour , Dong Tian

Speckle based imaging consists of forming a super-resolved reconstruction of an unknown sample from low-resolution images obtained under random inhomogeneous illuminations (speckles). In a blind context where the illuminations are unknown,…

Data Analysis, Statistics and Probability · Physics 2017-11-10 Jérôme Idier , Simon Labouesse , Marc Allain , Penghuan Liu , Sébastien Bourguignon , Anne Sentenac

Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical…

Though structured illumination (SI) microscopy is a popular imaging technique conventionally associated with fluorescent super-resolution, recent works have suggested its applicability towards sub-diffraction coherent imaging with…

Quantitative Methods · Quantitative Biology 2024-12-13 Shwetadwip Chowdhury , Will J. Eldridge , Adam Wax , Joseph A. Izatt

Structured illumination can reject out-of-focus signal from a sample, enabling high-speed and high-contrast imaging over large areas with widefield detection optics. Currently, this optical-sectioning technique is limited by image…