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In single-particle cryo-electron microscopy (cryo-EM), the efficient determination of orientation parameters for 2D projection images poses a significant challenge yet is crucial for reconstructing 3D structures. This task is complicated by…

Image and Video Processing · Electrical Eng. & Systems 2025-09-30 Szu-Chi Chung

Background and Objective: The contrast of cryo-EM images varies from one to another, primarily due to the uneven thickness of the ice layer. This contrast variation can affect the quality of 2-D class averaging, 3-D ab-initio modeling, and…

Computer Vision and Pattern Recognition · Computer Science 2022-07-01 Yunpeng Shi , Amit Singer

Typical diffusion models are trained to accept a particular form of conditioning, most commonly text, and cannot be conditioned on other modalities without retraining. In this work, we propose a universal guidance algorithm that enables…

Computer Vision and Pattern Recognition · Computer Science 2023-02-15 Arpit Bansal , Hong-Min Chu , Avi Schwarzschild , Soumyadip Sengupta , Micah Goldblum , Jonas Geiping , Tom Goldstein

In this paper we propose a modified cross correlation method to align images from the same class in single-particle electron microscopy of highly non-spherical structures. In this new method, First we coarsely align projection images, and…

Quantitative Methods · Quantitative Biology 2018-12-26 Wooram Park , Gregory S. Chirikjian

A simple, yet general, formalism for the optimized linear combination of astrophysical images is constructed and demonstrated. The formalism allows the user to combine multiple undersampled images to provide oversampled output at high…

Instrumentation and Methods for Astrophysics · Physics 2015-05-28 Barnaby Rowe , Christopher Hirata , Jason Rhodes

In this paper we study the formal algebraic structure underlying the intrinsic classification algorithm, recently introduced by Hadani, Shkolnisky, Singer and Zhao, for classifying noisy projection images of similar viewing directions in…

Representation Theory · Mathematics 2011-04-07 Ronny Hadani , Amit Singer

The group synchronization problem involves estimating a collection of group elements from noisy measurements of their pairwise ratios. This task is a key component in many computational problems, including the molecular reconstruction…

Signal Processing · Electrical Eng. & Systems 2022-12-09 Noam Janco , Tamir Bendory

The EM algorithm is a generic tool that offers maximum likelihood solutions when datasets are incomplete with data values missing at random or completely at random. At least for its simplest form, the algorithm can be rewritten in terms of…

Methodology · Statistics 2025-09-25 Daniel A. Griffith

This paper considers the non-linear inverse problem of reconstructing an electric conductivity distribution from the interior power density in a bounded domain. Applications include the novel tomographic method known as acousto-electric…

Optimization and Control · Mathematics 2018-08-29 B. J. Adesokan , Kim Knudsen , Venkateswaran P. Krishnan , Souvik Roy

Accurate orientation estimation is a crucial component of 3D molecular structure reconstruction, both in single-particle cryo-electron microscopy (cryo-EM) and in the increasingly popular field of cryo-electron tomography (cryo-ET). The…

Applications · Statistics 2026-02-25 Sheng Xu , Amnon Balanov , Amit Singer , Tamir Bendory

We develop a generalized inverse optimization framework for fitting the cost vector of a single linear optimization problem given multiple observed decisions. This setting is motivated by ensemble learning, where building consensus from…

Optimization and Control · Mathematics 2020-06-08 Aaron Babier , Timothy C. Y. Chan , Taewoo Lee , Rafid Mahmood , Daria Terekhov

Cryo-electron microscopy (cryo-EM) has recently emerged as a powerful tool for obtaining three-dimensional (3D) structures of biological macromolecules in native states. A minimum cryo-EM image data set for deriving a meaningful…

Applications · Statistics 2014-04-28 Ting-Li Chen , Dai-Ni Hsieh , Hung Hung , I-Ping Tu , Pei-Shien Wu , Yi-Ming Wu , Wei-Hau Chang , Su-Yun Huang

We introduce a new rotationally invariant viewing angle classification method for identifying, among a large number of Cryo-EM projection images, similar views without prior knowledge of the molecule. Our rotationally invariant features are…

Biomolecules · Quantitative Biology 2014-03-19 Zhizhen Zhao , Amit Singer

We describe an image compression method, consisting of a nonlinear analysis transformation, a uniform quantizer, and a nonlinear synthesis transformation. The transforms are constructed in three successive stages of convolutional linear…

Computer Vision and Pattern Recognition · Computer Science 2020-07-28 Johannes Ballé , Valero Laparra , Eero P. Simoncelli

Electron microscopy (EM) images exhibit anisotropic axial resolution due to the characteristics inherent to the imaging modality, presenting challenges in analysis and downstream tasks.In this paper, we propose a diffusion-model-based…

Computer Vision and Pattern Recognition · Computer Science 2023-08-04 Kyungryun Lee , Won-Ki Jeong

Single particle cryo-electron microscopy is a vital tool for 3D characterization of protein structures. A typical workflow involves acquiring projection images of a collection of randomly oriented particles, picking and classifying…

Image and Video Processing · Electrical Eng. & Systems 2021-03-22 S. V. Venkatakrishnan , Puneet Juneja , Hugh O'Neill

Addressing biases in AI models is crucial for ensuring fair and accurate predictions. However, obtaining large, unbiased datasets for training can be challenging. This paper proposes a comprehensive approach using multiple methods to remove…

Machine Learning · Computer Science 2024-02-15 Ahmed Radwan , Layan Zaafarani , Jetana Abudawood , Faisal AlZahrani , Fares Fourati

We study a class of mathematical and statistical algorithms with the aim of establishing a computer-based framework for fast and reliable automatic abnormality detection on landmark represented image templates. Under this framework, we…

Computer Vision and Pattern Recognition · Computer Science 2017-05-12 S. Huzurbazar , Long Lee , Dongyang Kuang

Cryo-Electron Microscopy (Cryo-EM) is a Nobel prize-winning technology for determining the 3D structure of particles at near-atomic resolution. A fundamental step in the recovering of the 3D single-particle structure is to align its 2D…

Image and Video Processing · Electrical Eng. & Systems 2021-01-12 Koby Bibas , Gili Weiss-Dicker , Dana Cohen , Noa Cahan , Hayit Greenspan

The number of noisy images required for molecular reconstruction in single-particle cryo-electron microscopy (cryo-EM) is governed by the autocorrelations of the observed, randomly-oriented, noisy projection images. In this work, we…

Numerical Analysis · Mathematics 2023-05-03 Tamir Bendory , Yuehaw Khoo , Joe Kileel , Oscar Mickelin , Amit Singer