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Fluorescence microscopy is widely used for the study of biological specimens. Deconvolution can significantly improve the resolution and contrast of images produced using fluorescence microscopy; in particular, Bayesian-based methods have…

Methodology · Statistics 2015-02-04 Alexander Wong , Xiao Yu Wang , Maud Gorbet

In multi-photon microscopy (MPM), a recent in-vivo fluorescence microscopy system, the task of image restoration can be decomposed into two interlinked inverse problems: firstly, the characterization of the Point Spread Function (PSF) and…

Image and Video Processing · Electrical Eng. & Systems 2024-11-08 Julien Ajdenbaum , Emilie Chouzenoux , Claire Lefort , Ségolène Martin , Jean-Christophe Pesquet

Deconvolution is the most commonly used image processing method to remove the blur caused by the point-spread-function (PSF) in optical imaging systems. While this method has been successful in deblurring, it suffers from several…

Image and Video Processing · Electrical Eng. & Systems 2019-10-10 Huangxuan Zhao , Ziwen Ke , Ningbo Chen , Ke Li , Lidai Wang , Xiaojing Gong , Wei Zheng , Liang Song , Zhicheng Liu , Dong Liang , Chengbo Liu

Optical microscopy is an essential tool in biology and medicine. Imaging thin, yet non-flat objects in a single shot (without relying on more sophisticated sectioning setups) remains challenging as the shallow depth of field that comes with…

Image and Video Processing · Electrical Eng. & Systems 2020-10-14 Adrian Shajkofci , Michael Liebling

We present a variational Bayesian method of joint image reconstruction and point spread function (PSF) estimation when the PSF of the imaging device is only partially known. To solve this semi-blind deconvolution problem, prior…

Data Analysis, Statistics and Probability · Physics 2013-03-18 Se Un Park , Nicolas Dobigeon , Alfred O. Hero

We present a semi-blind, spatially-variant deconvolution technique aimed at optical microscopy that combines a local estimation step of the point spread function (PSF) and deconvolution using a spatially variant, regularized Richardson-Lucy…

Computer Vision and Pattern Recognition · Computer Science 2019-05-21 Adrian Shajkofci , Michael Liebling

A non iterative direct blind deconvolution procedure, previously used successfully to sharpen Hubble Space Telescope imagery, is now found useful in sharpening nanoscale scanning electron microscope (SEM) and helium ion microscope (HIM)…

Instrumentation and Detectors · Physics 2025-02-28 Alfred S. Carasso , Andras E. Vladar

Super-resolution microscopes (such as STED) illuminate samples with a tiny spot, and achieve very high resolution. But structures smaller than the spot cannot be resolved in this way. Therefore, we propose a technique to solve this problem.…

Image and Video Processing · Electrical Eng. & Systems 2019-11-20 Yaohua Xie

The quality of microscopy images often suffers from optical aberrations. These aberrations and their associated point spread functions have to be quantitatively estimated to restore aberrated images. The recent state-of-the-art method…

Image and Video Processing · Electrical Eng. & Systems 2022-11-22 Kira Vinogradova , Eugene W. Myers

We propose a deconvolution algorithm for images blurred and degraded by a Poisson noise. The algorithm uses a fast proximal backward-forward splitting iteration. This iteration minimizes an energy which combines a \textit{non-linear} data…

Applications · Statistics 2008-12-18 François-Xavier Dupé , Jalal Fadili , Jean Luc Starck

Ultrasound is widely used in medical diagnostics allowing for accessible and powerful imaging but suffers from resolution limitations due to diffraction and the finite aperture of the imaging system, which restricts diagnostic use. The…

Computer Vision and Pattern Recognition · Computer Science 2024-08-08 Felix Duelmer , Walter Simson , Mohammad Farid Azampour , Magdalena Wysocki , Angelos Karlas , Nassir Navab

Reproducing an all-in-focus image from an image with defocus regions is of practical value in many applications, eg, digital photography, and robotics. Using the output of some existing defocus map estimator, existing approaches first…

Computer Vision and Pattern Recognition · Computer Science 2018-08-29 Guodong Xu , Chaoqiang Liu , Hui Ji

This Point spread function (PSF) plays a crucial role in many computational imaging applications, such as shape from focus/defocus, depth estimation, and fluorescence microscopy. However, the mathematical model of the defocus process is…

Computer Vision and Pattern Recognition · Computer Science 2022-09-20 Renzhi He , Yan Zhuang , Boya Fu , Fei Liu

A point-spread function describes the optics of an imaging system and can be used to correct collected images for instrumental effects. The state of the art for deconvolving images with the point-spread function is the Richardson-Lucy…

Instrumentation and Methods for Astrophysics · Physics 2024-05-07 Stefan Johann Hofmeister

Super-resolution techniques overcome the diffraction-limit and get very high resolutions. A category of these techniques, e.g., STED achieves this by creating an illumination spot smaller than the Airy Disk. As a result, points are…

Image and Video Processing · Electrical Eng. & Systems 2019-11-20 Yaohua Xie

We study the problem of deconvolution for light-sheet microscopy, where the data is corrupted by spatially varying blur and a combination of Poisson and Gaussian noise. The spatial variation of the point spread function (PSF) of a…

Richardson-Lucy deconvolution is widely used to restore images from degradation caused by the broadening effects of a point spread function and corruption by photon shot noise, in order to recover an underlying object. In practice, this is…

Computer Vision and Pattern Recognition · Computer Science 2024-11-05 Zachary H. Hendrix , Peter T. Brown , Tim Flanagan , Douglas P. Shepherd , Ayush Saurabh , Steve Pressé

This paper demonstrates a practical method that can correct spatial varying blur from a set of images of the same object. The algorithm jointly estimates the object and local point spread functions~(PSF). The method prioritizes sections…

Image and Video Processing · Electrical Eng. & Systems 2020-11-04 Wouter van de Ketterij , Oleg Soloviev , Michel Verhaegen

A major issue in optical astronomical image analysis is the combined effect of the instrument's point spread function (PSF) and the atmospheric seeing that blurs images and changes their shape in a way that is band and time-of-observation…

Instrumentation and Methods for Astrophysics · Physics 2024-10-02 Sreevarsha Sreejith , Anže Slosar , Hong Wang

Many microscopy applications are limited by the total amount of usable light and are consequently challenged by the resulting levels of noise in the acquired images. This problem is often addressed via (supervised) deep learning based…

Image and Video Processing · Electrical Eng. & Systems 2020-08-20 Anna S. Goncharova , Alf Honigmann , Florian Jug , Alexander Krull
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