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A method is proposed for assessing the temporal resolution of Structured Illumination Microscopy (SIM), by tracking the amplitude of different spatial frequency components over time, and comparing them to a temporally-oscillating…

Optics · Physics 2020-08-05 Abderrahim Boualam , Christopher J Rowlands

Structured light illumination is an active 3-D scanning technique based on projecting/capturing a set of striped patterns and measuring the warping of the patterns as they reflect off a target object's surface. In the case of phase…

Computer Vision and Pattern Recognition · Computer Science 2017-06-09 Daniel L. Lau , Yu Zhang , Kai Liu

Intensity correlation microscopy (ICM), which is prominently known through antibunching microscopy or super-resolution optical fluctuation imaging (SOFI), provides superresolution through a correlation analysis of antibunching of…

Optics · Physics 2018-10-08 Anton Classen , Joachim von Zanthier , Girish S. Agarwal

Fourier ptychographic (FP) microscope is a coherent imaging method that can synthesize an image with a higher bandwidth using multiple low-bandwidth images captured at different spatial frequency regions. The method's demand for multiple…

Optics · Physics 2016-03-25 Jaebum Chung , Hangwen Lu , Xiaoze Ou , Haojiang Zhou , Changhuei Yang

For quality control in the factory, 3D-metrology faces increasing demands for high precision and for more space-bandwidth-speed-product SBSP (number of 3D-points/sec). As a potential solution, we will discuss Structured-Illumination…

Optics · Physics 2023-07-19 Zheng Yang , Alexander Kessel , Gerd Häusler

We propose a chiral imaging modality based on optical chirality engineering, fluorescence-detected circular dichroism and structured illumination microscopy. In this method, the optical chirality of the illumination is structured and the…

Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for quantitative fluorescence imaging because fluorescence lifetime is independent of concentration of fluorescent molecules or excitation/detection efficiency and is robust…

Structured Illumination Microscopy (SIM) enables rapid, high-contrast optical sectioning of fresh tissue without staining or physical sectioning, making it promising for intraoperative and point-of-care diagnostics. Recent foundation and…

When one captures images in low-light conditions, the images often suffer from low visibility. This poor quality may significantly degrade the performance of many computer vision and multimedia algorithms that are primarily designed for…

Computer Vision and Pattern Recognition · Computer Science 2016-07-26 Xiaojie Guo

Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random…

Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two…

Image and Video Processing · Electrical Eng. & Systems 2022-01-19 Varun Mannam , Xiaotong Yuan , Scott Howard

Fast and sensitive detector arrays enable image scanning microscopy (ISM), overcoming the trade-off between spatial resolution and signal-to-noise ratio (SNR) typical of confocal microscopy. However, current ISM approaches cannot provide…

In most biological tissues, light scattering due to small differences in refractive index limits the depth of optical imaging systems. Two-photon microscopy (2PM), which significantly reduces the scattering of the excitation light, has…

Fourier Ptychographic Microscopy (FPM) is a computational technique that achieves a large space-bandwidth product imaging. It addresses the challenge of balancing a large field of view and high resolution by fusing information from multiple…

Optics · Physics 2023-04-21 Ruiqing Sun , Delong Yang , Yao Hu , Qun Hao , Xin Li , Shaohui Zhang

When capturing images in low-light conditions, the images often suffer from low visibility, which not only degrades the visual aesthetics of images, but also significantly degenerates the performance of many computer vision algorithms. In…

Computer Vision and Pattern Recognition · Computer Science 2020-12-17 Lijun Zhang , Xiao Liu , Erik Learned-Miller , Hui Guan

Confocal laser scanning microscopy (CLSM) stands out as one of the most widely used microscopy techniques, thanks to its three-dimensional imaging capability and its sub-diffraction spatial resolution, achieved through the closure of a…

Many optical microscopy techniques rely on structured illumination by way of a projected image (eg. structured illumination microscopy) or a tailored angular distribution of light (eg. ptychography). Electro-optical equipment such as…

Physics Education · Physics 2020-09-23 Antony Orth

We present a novel approach based on machine learning for designing photonic structures. In particular, we focus on strong light confinement that allows the design of an efficient free-space-to-waveguide coupler which is made of Si- slab…

Efficient fiber-to-waveguide light coupling has been a key issue in integrated photonics for many years. The main challenge lies in the huge mode mismatch between an optical fiber and a single mode waveguide. Herein, we present a novel…

Optics · Physics 2016-06-02 Zhaolin Lu , Peichuan Yin , Kaifeng Shi

Numerous modern technologies are reliant on the low-phase noise and exquisite timing stability of microwave signals. Substantial progress has been made in the field of microwave photonics, whereby low noise microwave signals are generated…