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Related papers: Quantitative phase imaging based on Fresnel diffra…

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We show that de-focused single particle images recorded using a cryo-electron microscope (cryoEM) system may be processed like a Fresnel zone in-line hologram to obtain physically meaningful quantitative phase maps associated with…

Image and Video Processing · Electrical Eng. & Systems 2021-11-24 Apoorv Pant , Manidipa Banerjee , Kedar Khare

Quantitative phase microscopy (QPM), a technique combining phase imaging and microscopy, enables visualization of the 3D topography in reflective samples, as well as the inner structure or refractive index distribution of transparent and…

Optics · Physics 2025-01-17 Vicente Mico , Juanjuan Zheng , Javier Garcia , Zeev Zalevsky , Peng Gao

Optical imaging plays a critical role in advancing our understanding of three dimensional dynamics of biological systems. Coherent imaging (CI) methods exploit spatial phase information, encoded through propagation of coherent signal light…

Optics · Physics 2015-07-24 Jeffrey J. Field , David G. Winters , Randy A. Bartels

We introduce a new modality for dynamic phase imaging in confocal microscopy based on synthetic optical holography. By temporal demultiplexing of the detector signal into a series of holograms, we record time-resolved phase images directly…

Optics · Physics 2019-04-09 Martin Schnell , P. Scott Carney , Rainer Hillenbrand

Quantitative phase imaging (QPI) recovers the exact wavefront of light from the intensity measured by a camera. Topographical maps of translucent microscopic bodies can be extracted from these quantified phase shifts. We demonstrate…

Sensing with undetected photons allows access to spectral regions with simultaneous detection of photons of another region and is based on nonlinear interferometry. To obtain the full information of a sample, the corresponding interferogram…

Reflection phase imaging provides label-free, high-resolution characterization of biological samples, typically using interferometric-based techniques. Here, we investigate reflection phase microscopy from intensity-only measurements under…

Quantitative Methods · Quantitative Biology 2019-12-18 Alex Matlock , Anne Sentenac , Patrick C. Chaumet , Ji Yi , Lei Tian

A simple method of phase-and-amplitude extraction is derived that corrects for image blurring induced by partially spatially coherent incident illumination using only a single intensity image as input. The method is based on Fresnel…

Optics · Physics 2019-11-19 Mario A. Beltran , David M. Paganin , Daniele Pelliccia

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…

Quantum imaging with undetected light (QIUL) can retrieve amplitude and phase information of an object by exploiting the quantum correlations of photon-pairs generated through spontaneous parametric down conversion (SPDC), where the…

In the last five decades, iterative phase retrieval methods draw large amount of interest across the research community as a non-interferometric approach to recover quantitative phase distributions from one (or more) intensity measurement.…

Optics · Physics 2020-07-21 Nathaniel Hai , Joseph Rosen

We propose and demonstrate a new phase retrieval method for imaging through random media. Although methods to recover the Fourier amplitude through random distortions are well established, recovery of the Fourier phase has been a more…

Optics · Physics 2021-05-14 Byungjae Hwang , Taeseong Woo , Cheolwoo Ahn , Jung-Hoon Park

Recovering both amplitude and phase information from a system is a fundamental goal of optical imaging. At the same time, it is crucial to operate at low photon doses to avoid altering the sample, particularly in biological applications.…

Quantum Physics · Physics 2026-01-15 Alberto Paniate , Giuseppe Ortolano , Sarika Soman , Marco Genovese , Ivano Ruo-Berchera

Imaging transparent samples remains an ongoing challenge in the study of unstained biological cells and material samples. Widely used methods trade off system complexity, cost and bulk, computational efficiency and information content. Here…

Phase microscopy is an invaluable tool in the biosciences and in clinical diagnostics. The sensitivity of current phase microscopy techniques is optimized for one specific mean phase value and varies significantly across a given sample.…

Optics · Physics 2019-08-30 Thomas Juffmann , Andrés de los Ríos Sommer , Sylvain Gigan

In most theories of diffraction by a diaphragm, the amplitude of the diffracted wave, and hence the position wave function of the associated particle, is calculated directly without prior calculation of the quantum state. Few models express…

Quantum Physics · Physics 2024-10-29 Bernard Fabbro

Diffractive surfaces shape optical wavefronts for applications in spectroscopy, high-speed communication, and imaging. The performance of these structures is primarily determined by how precisely they can be patterned. Fabrication…

Various methods have been introduced to measure the orbital angular momentum (OAM) of light, from fork holograms to Dove prism interferometers, from tilted lenses to triangular apertures - each with their own benefits and limitations. Here…

In computational phase imaging with a microscope equipped with an array of light emitting diodes as illumination unit, conventional Fourier ptychographic microscopy achieves high resolution and wide-field reconstructions but is constrained…