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Related papers: Multi-purpose SLM-light-sheet microscope

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Among super-resolution microscopy techniques, structured illumination microscopy (SIM) shows great advances of low phototoxicity, high speed, and excellent performance in long-term dynamic observation, making it especially suitable for live…

Optics · Physics 2025-02-07 Han Wang , Wenshu Wang , Xinzhu Xu , Meiqi Li , Peng Xi

We developed a scan mirror mechanism (SMM) that enable a slit-based spectrometer or spectropolarimeter to precisely and quickly map an astronomical object. The SMM, designed to be installed in the optical path preceding the entrance slit,…

We propose and demonstrate a new imaging technique to noninvasively see through scattering layers with the aid of a spatial light modulator (SLM). A relay system projects the incoherent light pattern emitting from the scattering layer onto…

Optics · Physics 2019-05-30 Saswata Mukherjee , A. Vijayakumar , Joseph Rosen

We develop a novel hyperspectral imaging system using structured illumination in an SLM-based Michelson interferometer. In our design, we use a reflective SLM as a mirror in one of the arms of a Michelson interferometer, and scan the…

Optics · Physics 2022-04-25 Amar Deo Chandra , Mintu Karmakar , Dibyendu Nandy , Ayan Banerjee

We developed a Bessel light sheet fluorescence microscopy (LSFM) system to enable high-speed, wide-field intra-vital imaging of zebrafish and other thick biological samples. This system uses air objectives for the convenient mounting of…

Optics · Physics 2023-11-07 Chuhui Wang , Jiaju Chen , Cuiyi Peng , Zhenglin Chen , Dongmei Yu , Peiwu Qin

Spatial light modulators (SLMs) are devices for modulating amplitude, phase or polarization of a light beam on demand. Such devices have been playing an indispensable inuence in many areas from our daily entertainments to scientific…

Optics · Physics 2020-03-12 Shengchao Gong , Mengxin Ren , Wei Wu , Wei Cai , Jingjun Xu

The on-demand tailoring of light's spatial shape is of great relevance in a wide variety of research areas. Computer-controlled devices, such as Spatial Light Modulators (SLMs) or Digital Micromirror Devices (DMDs), offer a very accurate,…

Compact, lightweight and high-performance spatial light modulators (SLMs) are crucial for modern optical technologies. The drive for pixel miniaturization, necessary to improve their performance, has led to a promising alternative, active…

Sub-diffraction resolution, gentle sample illumination, and the possibility to image in multiple colors make Structured Illumination Microscopy (SIM) an imaging technique which is particularly well suited for live cell observations. Here,…

In this paper, a compact and low-cost structured illumination microscope (SIM) based on a 2X2 fiber coupler is presented. Fringe illumination is achieved by placing two output fiber tips at a conjugate Fourier plane of the sample plane as…

Instrumentation and Detectors · Physics 2018-12-19 Shiming Hu , Lei Liu , Yizheng Huang , Wenwen Liu , Qingquan Wei , Manqing Tan , Yude Yu

We present a comprehensive guide to light-sheet microscopy (LSM) to assist scientists in navigating the practical implementation of this microscopy technique. Emphasizing the applicability of LSM to image both static microscale and…

Modulation-enhanced localization microscopy (MELM) has demonstrated significant improvements in both lateral and axial localization precision compared to conventional single-molecule localization microscopy (SMLM). However, lateral…

Quantitative imaging of subcellular processes in living embryos, stem-cell systems, and organoid models requires microscopy platforms that combine high spatial resolution, fast volumetric acquisition, long-term stability, and minimal…

Quantitative Methods · Quantitative Biology 2025-12-30 Armin Shoushtarizadeh , Michele Cerminara , Corinne Chureau , Leah Friedman , Deepthi Kailash , Thomas Gregor

High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy. Here, we use…

Image and Video Processing · Electrical Eng. & Systems 2019-03-28 Li-Hao Yeh , Shwetadwip Chowdhury , Laura Waller

In wide-field nonlinear microscopy, wavefront modulation by means of phase-only spatial light modulators (SLMs) allows achieving simultaneous two-photon excitation and fluorescence emission from specific region-of-interests (ROIs) of…

Structured illumination microscopy (SIM) is a very important super-resolution microscopy technique, which provides high speed super-resolution with about two-fold spatial resolution enhancement. Several attempts aimed at improving the…

Computer Vision and Pattern Recognition · Computer Science 2016-02-23 Amit Lal , Chunyan Shan , Peng Xi

Rapidly developing augmented reality (AR) and 3D holographic display technologies require spatial light modulators (SLM) with high resolution and viewing angle to be able to satisfy increasing customer demands. Currently available SLMs, as…

Reconstituted cytoskeleton composites have emerged as a valuable model system for studying non equilibrium soft matter. Faithful capture of the dynamics of these three dimensional, dense networks calls for optical sectioning often…

Instrumentation and Detectors · Physics 2023-05-01 Nathan Felcher , Daisy Achiriloaie , Brian Lee , Ryan McGorty , Janet Sheung

We present experimental demonstration of tilt-mirror assisted transmission structured illumination microscopy (tSIM) that offers a large field of view super resolution imaging. An assembly of custom-designed tilt-mirrors are employed as the…

Programmable optical devices provide performance enhancement and flexibility to spatial multiplexing systems enabling transmission of tributaries in high-order eigenmodes of spatially-diverse transmission media, like multimode fiber (MMF).…