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The wavelength calibration of spectrographs is an essential but challenging task in many disciplines. Calibration is traditionally accomplished by imaging the spectrum of a light source containing features that are known to appear at…

Instrumentation and Methods for Astrophysics · Physics 2018-04-04 Neil Thomas

Fluorescence imaging is indispensable to biology and neuroscience. The need for large-scale imaging in freely behaving animals has further driven the development in miniaturized microscopes (miniscopes). However, conventional microscopes /…

Optics · Physics 2020-10-29 Yujia Xue , Ian G. Davison , David A. Boas , Lei Tian

We demonstrate the possibility of a self-consistent characterization of the photon-number statistics of a light field by using photoemissive detectors with internal gain simply endowed with linear input/output responses. The method can be…

Quantum Physics · Physics 2015-05-13 Maria Bondani , Alessia Allevi , Andrea Agliati , Alessandra Andreoni

The propagation of errors through the uniform disk visibility function is examined. Implications of those errors upon measures of absolute visibility through optical and near-infrared interferometers are considered within the context of…

Astrophysics · Physics 2009-11-11 Gerard T. van Belle , Gerald van Belle

We describe the optimum telescope focal ratio for a two-element, three surface, telecentric image-transfer microlens-to-fiber coupled integral field unit within the constraints imposed by micro-optics fabrication and optical aberrations. We…

Instrumentation and Methods for Astrophysics · Physics 2022-05-09 Sabyasachi Chattopadhyay , Matthew A. Bershady , Marsha J. Wolf , Michael P. Smith

The complexity and accuracy of current and future precision cosmology observational campaigns has made it essential to develop an efficient technique for directly combining simulation and observational datasets to determine cosmological and…

Astrophysics · Physics 2009-11-11 Katrin Heitmann , David Higdon , Charles Nakhleh , Salman Habib

We describe the effect of optical aberrations on fluorescence fluctuations microscopy (FFM), when focusing through a single living cell. FFM measurements are performed in an aqueous fluorescent solution and prove to be a highly sensitive…

Biological Physics · Physics 2014-03-13 Charles-Edouard Leroux , Alexei Grichine , Irène Wang , Antoine Delon

Robust and accurate camera calibration is essential for 3D reconstruction in light microscopy under circular motion. Conventional methods require either accurate key point matching or precise segmentation of the axial-view images. Both…

Computer Vision and Pattern Recognition · Computer Science 2019-10-31 Yuanhao Guo , Fons J. Verbeek , Ge Yang

This paper describes the setup of two different solutions for laboratory X-ray microscopy working with geometric magnification. One setup uses thin-film transmission targets with an optimized tungsten-layer thickness and the electron gun…

Instrumentation and Detectors · Physics 2015-06-11 Thomas Ebensperger , Philipp Stahlhut , Frank Nachtrab , Simon Zabler , Randolf Hanke

Light sheet fluorescence microscopy provides optical sectioning and is widely used in volumetric imaging of large specimens. However, the axial resolution and the lateral Field of View (FoV) of the system, defined by the light sheet,…

Optics · Physics 2021-06-16 Hongjin Li , Zihan Wu , Zhichao Yang , Karl Zhanghao , Peng Xi , Dayong Jin

Aberration correctors are essential elements for achieving atomic resolution in state-of-the-art electron microscopes. Conventional correctors are based on a series of multipolar electron lenses, but more versatile alternatives are…

Optics · Physics 2025-01-29 Zdeněk Nekula , Thomas Juffmann , Andrea Konečná

In this paper are reviewed various designs of advanced, multi-aperture optical systems dedicated to high angular resolution imaging or to the detection of exo-planets by nulling interferometry. A simple Fourier optics formalism applicable…

Optics · Physics 2016-01-20 Francois Henault

In a line caustic crossing microlensing event, the caustic line moving across the surface of the source star provides a direct method to measure the integrated luminosity profile of the star. Combined with the enormous brightening at the…

Astrophysics · Physics 2007-05-23 Sun Hong Rhie , David P. Bennett

Three-dimensional direct laser writing via two photon polymerization is used to fabricate anti-reflective structured surfaces composed of sub-wavelength conicoid features optimized to operate over a wide bandwidth in the near-infrared range…

Optics · Physics 2018-01-08 Y. Li , D. B. Fullager , E. Angelbello , D. Childers , G. Boreman , T. Hofmann

We present a novel learning-based method to build a differentiable computational model of a real fluorescence microscope. Our model can be used to calibrate a real optical setup directly from data samples and to engineer point spread…

Image and Video Processing · Electrical Eng. & Systems 2023-06-12 Josue Page , Paolo Favaro

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

Optics · Physics 2022-01-26 Yi Xue , David Ren , Laura Waller

We quantify the performance of mass mapping techniques on mock imaging and gravitational lensing data of galaxy clusters. The optimum method depends upon the scientific goal. We assess measurements of clusters' radial density profiles,…

Cosmology and Nongalactic Astrophysics · Physics 2020-07-29 Sut-Ieng Tam , Richard Massey , Mathilde Jauzac , Andrew Robertson

An efficient method to tune the spatial coherence of a degenerate laser over a broad range with minimum variation in the total output power is presented. It is based on varying the diameter of a spatial filter inside the laser cavity. The…

Optics · Physics 2015-06-16 Micha Nixon , Brandon Redding , Asher Friesem , Hui Cao , Nir Davidson

Astrometric observations of microlensing events were originally proposed to determine the lens proper motion with which the physical parameters of lenses can be better constrained. In this proceeding, we demonstrate that besides this…

Astrophysics · Physics 2007-05-23 Cheongho Han