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Digital holographic microscopy is a thriving imaging modality that attracted considerable research interest in quantitative biological cell imaging due to its ability to not only create excellent label-free contrast, but also supply…

Optics · Physics 2019-04-03 Gili Dardikman , Natan T. Shaked

Digital holography has applications in bio-imaging because it can simultaneously obtain the amplitude and phase information of a microscopic sample in a single shot, thus facilitating non-contact, noninvasive observation of the 3D shape of…

Graphics · Computer Science 2024-06-14 Yuki Nagahama

Automated label-free quantitative imaging of biological samples can greatly benefit high throughput diseases diagnosis. Digital holographic microscopy (DHM) is a powerful quantitative label-free imaging tool that retrieves structural…

We suggest a new multimodal imaging technique for quantitatively measuring the integral (thickness-average) refractive index of the nuclei of live biological cells in suspension. For this aim, we combined quantitative phase microscopy with…

Holotomography, a three-dimensional quantitative phase imaging technique, presents an innovative, non-invasive approach to studying biological samples by exploiting the refractive index as an intrinsic imaging contrast. Despite offering…

Optics · Physics 2023-08-04 Herve Hugonnet , HyunJun Han , Weisun Park , YongKeun Park

Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy…

The refractive index (RI) of cells and tissues is crucial in pathophysiology as a noninvasive and quantitative imaging contrast. Although its measurements have been demonstrated using three-dimensional quantitative phase imaging methods,…

Intravital microscopy is an essential tool that reveals behaviours of live cells under conditions close to natural physiological states. So far, although various approaches for imaging cells in vivo have been proposed, most require the use…

Biological Physics · Physics 2016-07-04 Kyoohyun Kim , Kibaek Choe , Inwon Park , Pilhan Kim , YongKeun Park

Manual operation of microscopes for repetitive tasks in cell biology is a significant bottleneck, consuming invaluable expert time, and introducing human error. Automation is essential, and while Digital Holographic Microscopy (DHM) offers…

We developed a microscopy technique that can measure the local refractive index without sampling the optical phase delay of the electromagnetic radiation. To do this, we designed and experimentally demonstrated a setup with two co-localized…

Optics · Physics 2019-03-27 Antonio Fiore , Carlo Bevilacqua , Giuliano Scarcelli

We present a novel differential refractive index sensor based on arrays of photonic molecules (PM) of dielectric cylinders and two structural defect cavities. The transmission spectrum of the photonic proposed structure as sensor shows a…

Optics · Physics 2016-08-09 Angel Andueza , Jesus Perez-Conde , Joaquin Sevilla

Refractive index is a fundamental optical property of powder and a key input to the measurement of the size distribution using light scattering and the measurement of the absorption and scattering coefficients using diffuse reflectance…

Optics · Physics 2024-03-05 Cory Juntunen , Adam J. Rish , Carl Anderson , Yongjin Sung

Non-contact measurement of the refractive index and thickness of multilayer biological tissues is of great significance for biomedical applications and can greatly improve medical diagnosis and treatment. In this work, we introduce a…

Optics · Physics 2024-04-19 Yu-Lin Ku , Yao-Gen Shu

We present a cost-effective, simple and fast digital holographic microscopy method based upon Rayleigh-Sommerfeld back propagation for identification of the geometrical shape of a cell. The method was tested using synthetic hologram images…

Optics · Physics 2015-10-28 Johan Zakrisson , Staffan Schedin , Magnus Andersson

This industrial Ph.D. project, carried out in collaboration between Radiometer Medical ApS and SDU Centre for Photonics Engineering at the University of Southern Denmark, explored the use of digital holographic microscopy (DHM) for the…

Optics · Physics 2026-02-10 Andreas Erik Gejl Madsen

Photopolymerization enables the production of micro-optical elements, such as diffractive optical elements or GRIN optics. This process utilizes targeted spatial modulation of the refractive index, which is achieved through additive…

Optics · Physics 2026-03-16 Andreas Heinrich , Manuel Rank

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

Optics · Physics 2022-01-26 Yi Xue , David Ren , Laura Waller

Optical diffraction tomography (ODT) is an interferometric microscopy technique capable of measuring 3-D refractive index (RI) distribution of transparent samples. Multiple 2-D holograms of a sample illuminated with various angles are…

The digital holographic interferometry (DHI) was utilized for investigating the optical properties of polymer and optical fibers. The samples investigated here were polyvinylidene fluoride (PVDF) polymer fiber and graded-index (GRIN)…

Pixel size in cameras and other refractive imaging devices is typically limited by the free-space diffraction. However, a vast majority of semiconductor-based detectors are based on materials with substantially high refractive index. We…

Optics · Physics 2018-03-28 Bo Fan , Sandeep Inampudi , Viktor A. Podolskiy
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