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Related papers: Deep-STORM: super-resolution single-molecule micro…

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Despite super-resolution fluorescence blinking microscopes break the diffraction limit, the intense phototoxic illumination and long-term image sequences thus far still pose to major challenges in visualizing live-organisms. Here, we…

Structured illumination microscopy (SIM) reconstructs a super-resolved image from multiple raw images captured with different illumination patterns; hence, acquisition speed is limited, making it unsuitable for dynamic scenes. We propose a…

Optics · Physics 2025-06-17 Ruiming Cao , Fanglin Linda Liu , Li-Hao Yeh , Laura Waller

Raman spectroscopy enables non-destructive, label-free imaging with unprecedented molecular contrast but is limited by slow data acquisition, largely preventing high-throughput imaging applications. Here, we present a comprehensive…

Image and Video Processing · Electrical Eng. & Systems 2021-12-02 Conor C. Horgan , Magnus Jensen , Anika Nagelkerke , Jean-Phillipe St-Pierre , Tom Vercauteren , Molly M. Stevens , Mads S. Bergholt

Quantifying the number of molecules from fluorescence microscopy measurements is an important topic in cell biology and medical research. In this work, we present a consecutive algorithm for super-resolution (STED) scanning microscopy that…

Applications · Statistics 2023-10-03 Katharina Proksch , Frank Werner , Jan Keller-Findeisen , Haisen Ta , Axel Munk

We report resolution enhancement in scanning electron microscopy (SEM) images using a generative adversarial network. We demonstrate the veracity of this deep learning-based super-resolution technique by inferring unresolved features in…

Computer Vision and Pattern Recognition · Computer Science 2019-08-21 Kevin de Haan , Zachary S. Ballard , Yair Rivenson , Yichen Wu , Aydogan Ozcan

Magnetic Resonance Imaging (MRI) is important in clinic to produce high resolution images for diagnosis, but its acquisition time is long for high resolution images. Deep learning based MRI super resolution methods can reduce scan time…

Image and Video Processing · Electrical Eng. & Systems 2022-09-08 Ziyan Lin , Zihao Chen

We demonstrate the use of deep learning for fast spectral deconstruction of speckle patterns. The artificial neural network can be effectively trained using numerically constructed multispectral datasets taken from a measured spectral…

Image and Video Processing · Electrical Eng. & Systems 2019-07-16 Ulas Kürüm , P. R. Wiecha , Rebecca French , Otto L. Muskens

In some super-resolution techniques, adjacent points are illuminated at different times. Thereby, their locations and light intensities can be detected even if the images are very blurred due to diffraction. According to conventional…

Image and Video Processing · Electrical Eng. & Systems 2019-12-10 Edward Y. Sheffield

Single-molecule localization microscopy techniques, like stochastic optical reconstruction microscopy (STORM), visualize biological specimens by stochastically exciting sparse blinking emitters. The raw images suffer from unwanted…

Computer Vision and Pattern Recognition · Computer Science 2024-01-17 Patris Valera , Josué Page Vizcaíno , Tobias Lasser

Tracking single molecules is instrumental for quantifying the transport of molecules and nanoparticles in biological samples, e.g., in brain drug delivery studies. Existing intensity-based localisation methods are not developed for imaging…

Polarized light microscopy provides high contrast to birefringent specimen and is widely used as a diagnostic tool in pathology. However, polarization microscopy systems typically operate by analyzing images collected from two or more light…

Volumetric imaging by fluorescence microscopy is often limited by anisotropic spatial resolution from inferior axial resolution compared to the lateral resolution. To address this problem, here we present a deep-learning-enabled…

Computer Vision and Pattern Recognition · Computer Science 2022-07-06 Hyoungjun Park , Myeongsu Na , Bumju Kim , Soohyun Park , Ki Hean Kim , Sunghoe Chang , Jong Chul Ye

Background: Single-particle cryo-electron microscopy (cryo-EM) has become a popular tool for structural determination of biological macromolecular complexes. High-resolution cryo-EM reconstruction often requires hundreds of thousands of…

Data Analysis, Statistics and Probability · Physics 2017-09-05 Yanan Zhu , Qi Ouyang , Youdong Mao

In order to address the issue that medical image would suffer from severe blurring caused by the lack of high-frequency details in the process of image super-resolution reconstruction, a novel medical image super-resolution method based on…

Computer Vision and Pattern Recognition · Computer Science 2019-05-14 Kewen Liu , Yuan Ma , Hongxia Xiong , Zejun Yan , Zhijun Zhou , Panpan Fang , Chaoyang Liu

Single-beam super-resolution microscopy, also known as superlinear microscopy, exploits the nonlinear response of fluorescent probes in confocal microscopy. The technique requires no complex purpose-built system, light field modulation, or…

With super-resolution optical microscopy, it is now possible to observe molecular interactions in living cells. The obtained images have a very high spatial precision but their overall quality can vary a lot depending on the structure of…

Computer Vision and Pattern Recognition · Computer Science 2018-03-30 Louis-Émile Robitaille , Audrey Durand , Marc-André Gardner , Christian Gagné , Paul De Koninck , Flavie Lavoie-Cardinal

Quantitative phase imaging (QPI) has been widely applied in characterizing cells and tissues. Spatial light interference microscopy (SLIM) is a highly sensitive QPI method, due to its partially coherent illumination and common path…

Image and Video Processing · Electrical Eng. & Systems 2024-06-12 Yuheng Jiao , Yuchen R. He , Mikhail E. Kandel , Xiaojun Liu , Wenlong Lu , Gabriel Popescu

We propose a state-of-the-art method for super-resolution with non-uniform blur. Single-image super-resolution methods seek to restore a high-resolution image from blurred, subsampled, and noisy measurements. Despite their impressive…

Computer Vision and Pattern Recognition · Computer Science 2024-05-24 Charles Laroche , Andrés Almansa , Matias Tassano

Cryo-electron microscopy (cryo-EM) has achieved near-atomic level resolution of biomolecules by reconstructing 2D micrographs. However, the resolution and accuracy of the reconstructed particles are significantly reduced due to the…

Computer Vision and Pattern Recognition · Computer Science 2024-01-03 Jing Zhang , Tengfei Zhao , ShiYu Hu , Xin Zhao

Recent advances in scanning transmission electron and scanning probe microscopies have opened exciting opportunities in probing the materials structural parameters and various functional properties in real space with angstrom-level…