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We report the development and detailed calibration of a multiphoton fluorescence lifetime imaging system (FLIM) using a streak camera. The present system is versatile with high spatial (0.2 micron) and temporal (50 psec) resolution and…

Quantitative Methods · Quantitative Biology 2009-11-10 R. V. Krishnan , H. Saitoh , H. Terada , V. E. Centonze , B. Herman

We present a physics-informed deep learning framework to address common limitations in Confocal Laser Scanning Microscopy (CLSM), such as diffraction limited resolution, noise, and undersampling due to low laser power conditions. The…

Materials Science · Physics 2025-01-27 Zaheer Ahmad , Junaid Shabeer , Usman Saleem , Tahir Qadeer , Abdul Sami , Zahira El Khalidi , Saad Mehmood

Compressive sensing (CS), aiming to reconstruct an image/signal from a small set of random measurements has attracted considerable attentions in recent years. Due to the high dimensionality of images, previous CS methods mainly work on…

Computer Vision and Pattern Recognition · Computer Science 2018-02-01 Xiaotong Lu , Weisheng Dong , Peiyao Wang , Guangming Shi , Xuemei Xie

Deep learning methods have witnessed the great progress in image restoration with specific metrics (e.g., PSNR, SSIM). However, the perceptual quality of the restored image is relatively subjective, and it is necessary for users to control…

Computer Vision and Pattern Recognition · Computer Science 2019-08-21 Wei Wang , Ruiming Guo , Yapeng Tian , Wenming Yang

We have developed an automatic method for segmenting fluorescence lifetime (FLT) imaging microscopy (FLIM) images of cells inspired by a multi-resolution community detection (MCD) based network segmentation method. The image processing…

Medical Physics · Physics 2014-01-06 Dandan Hu , Pinaki Sarder , Peter Ronhovde , Sandra Orthaus , Samuel Achilefu , Zohar Nussinov

Optical imaging plays a critical role in advancing our understanding of three dimensional dynamics of biological systems. Coherent imaging (CI) methods exploit spatial phase information, encoded through propagation of coherent signal light…

Optics · Physics 2015-07-24 Jeffrey J. Field , David G. Winters , Randy A. Bartels

Fluorescence microscopy is essential to study biological structures and dynamics. However, existing systems suffer from a tradeoff between field-of-view (FOV), resolution, and complexity, and thus cannot fulfill the emerging need of…

Optics · Physics 2022-09-09 Yujia Xue , Qianwan Yang , Guorong Hu , Kehan Guo , Lei Tian

Low-Light Image Enhancement (LLIE) is a key task in computational photography and imaging. The problem of enhancing images captured during night or in dark environments has been well-studied in the computer vision literature. However,…

Computer Vision and Pattern Recognition · Computer Science 2026-01-29 Juan C. Benito , Daniel Feijoo , Alvaro Garcia , Marcos V. Conde

Fluorescence microscopy has been a significant tool to observe long-term imaging of embryos (in vivo) growth over time. However, cumulative exposure is phototoxic to such sensitive live samples. While techniques like light-sheet…

Image and Video Processing · Electrical Eng. & Systems 2022-01-05 Varun Mannam , Jacob Brandt , Cody J. Smith , Scott Howard

Single-pixel imaging has emerged as a key technique in fluorescence microscopy, where fast acquisition and reconstruction are crucial. In this context, images are reconstructed from linearly compressed measurements. In practice, total…

Image and Video Processing · Electrical Eng. & Systems 2025-07-28 Serban C. Tudosie , Valerio Gandolfi , Shivaprasad Varakkoth , Andrea Farina , Cosimo D'Andrea , Simon Arridge

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

Fluorescence molecular tomography (FMT) is a promising modality for non-invasive imaging of internal fluorescence agents in biological tissues especially in small animal models, with applications in diagnosis, therapy, and drug design. In…

Optics · Physics 2023-08-09 Wenxiang Cong , Ge Wang

Compressed streak imaging (CSI) is a computational imaging strategy that can acquire video at over 150 trillion frames per second. Despite this achievement, CSI faces challenges in detecting subtle intensity fluctuations in slow-moving,…

Compressive sensing (CS) is an effective approach for fast Magnetic Resonance Imaging (MRI). It aims at reconstructing MR images from a small number of under-sampled data in k-space, and accelerating the data acquisition in MRI. To improve…

Computer Vision and Pattern Recognition · Computer Science 2017-05-22 Yan Yang , Jian Sun , Huibin Li , Zongben Xu

Fluorescence microscopy is widely employed for the analysis of living biological samples; however, the utility of the resulting recordings is frequently constrained by noise, temporal variability, and inconsistent visualisation of signals…

Computer Vision and Pattern Recognition · Computer Science 2026-01-16 Hassan Eshkiki , Sarah Costa , Mostafa Mohammadpour , Farinaz Tanhaei , Christopher H. George , Fabio Caraffini

Fluorescence microscopy has enabled a dramatic development in modern biology by visualizing biological organisms with micrometer scale resolution. However, due to the diffraction limit, sub-micron/nanometer features are difficult to…

Image and Video Processing · Electrical Eng. & Systems 2021-03-10 Varun Mannam , Yide Zhang , Xiaotong Yuan , Scott Howard

Magnetic resonance imaging (MRI) is a non-invasive imaging modality and provides comprehensive anatomical and functional insights into the human body. However, its long acquisition times can lead to patient discomfort, motion artifacts, and…

Computer Vision and Pattern Recognition · Computer Science 2025-07-21 Mojtaba Safari , Zach Eidex , Chih-Wei Chang , Richard L. J. Qiu , Xiaofeng Yang

Convolutional neural networks (CNNs) have gained tremendous success in solving complex inverse problems. The aim of this work is to develop a novel CNN framework to reconstruct video sequence of dynamic live cells captured using a…

Computer Vision and Pattern Recognition · Computer Science 2020-10-29 Thanh Nguyen , Yujia Xue , Yunzhe Li , Lei Tian , George Nehmetallah

Recently, DNN models for lossless image coding have surpassed their traditional counterparts in compression performance, reducing the previous lossless bit rate by about ten percent for natural color images. But even with these advances,…

Image and Video Processing · Electrical Eng. & Systems 2025-07-23 Xi Zhang , Xiaolin Wu

Due to its specificity, fluorescence microscopy (FM) has become a quintessential imaging tool in cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit FM's utility. Recently, it has been shown that…