Related papers: Brillouin microscopy, what is it really measuring?
Characterizing the micromechanical properties of cells and extracellular matrices is critical in mechanobiology. To meet this need, Brillouin light scattering (BLS) has emerged as a noncontact, high-resolution elastography tool that probes…
The role and importance of mechanical properties of cells and tissues in cellular function, development as well as disease has widely been acknowledged, however standard techniques currently used to assess them exhibit intrinsic…
Hydrogels are attractive and versatile gel-based structures consisting of three-dimensional networks of hydrophilic polymers with unique properties that can retain a large amount of water and be tailor-designed according to specific…
Brillouin light scattering spectroscopy (BLS) is applied to study the micromechanics of cellulosic viscose fibers, one of the commercially most important, man-made biobased fibers. Using an equal angle scattering geometry, we provide a…
Many problems in mechanobiology urgently require characterisation of the micromechanical properties of the fibrous proteins of cells and tissues. Brillouin light scattering has been proposed as a new optical elastography technique to meet…
Load-bearing tissues are typically fortified by networks of protein fibers, often with preferential orientations. This fiber structure imparts the tissues with direction-dependent mechanical properties optimized to support specific external…
Probing forces, deformations and generally speaking the mechanical properties of cells is the hallmark of mechanobiology. In the last two decades many techniques have been developed to this end that are largely based on deforming the cells…
Brillouin Light Scattering is a powerful technique to measure the microwave excitations present in a magnetic system. In microfocused mode, the light is focused on the sample using a microscope objective. This accelerates substantially the…
Brillouin microscopy is an emerging optical elastography technique that can be used to assess mechanical properties of biological samples in a 3D, all-optical and hence non-contact fashion. However, the low cross-section of spontaneous…
We use optical trapping to continuously bend an isolated microtubule while simultaneously measuring the applied force and the resulting filament strain, thus allowing us to determine its elastic properties over a wide range of applied…
We describe an easy-to-implement technique that allows for a complete experimental characterization of sub-wavelength diameter tapered optical fibers. Our method is based on a direct and fast numerical analysis of the backward Brillouin…
Brillouin scattering has applications ranging from signal processing, sensing and microscopy, to quantum information and fundamental science. Most of these applications rely on the electrostrictive interaction between light and phonons.…
Brillouin spectroscopy is commonly used to study the acoustic properties of materials. Here we explored its feasibility in studying the photoinduced isomerization of azobenzene. The isomerization of azobenzene changes the solution elastic…
Mass density is a vital property for improved biophysical understanding of and within biological samples. It is increasingly attracting active investigation, but still lacks reliable, non-contact techniques to accurately characterize it in…
Brillouin microscopy is an emerging technique for all-optical biomechanical imaging without the need for physical contact with the sample or for an external mechanical stimulus. However, Brillouin microscopy often retrieves a single,…
Brillouin microscopy is rapidly emerging as a powerful technique for imaging the mechanical properties of biological specimens in a label-free, non-contact manner. We present a standardized file format and open-source tools to facilitate…
Microrheology, the study of fluids on micron length-scales, promises to reveal insights into cellular biology, including mechanical biomarkers of disease and the interplay between biomechanics and cellular function. Here a…
Characterizing the elastic properties of soft materials through bulge testing relies on accurate measurement of deformation, which is experimentally challenging. To avoid measuring deformation, we propose a hydrodynamic bulge test for…
We present a straightforward method for measuring the fluids' relative viscosity via a simple graphical analysis of the normalised position autocorrelation function of an optically trapped bead, without the need of embarking on laborious…
Microrheology is a branch of rheology having the same principles as conventional bulk rheology, but working on micron length scales and micro-litre volumes. Optical tweezers have been successfully used with Newtonian fluids for rheological…