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Point spread function (PSF) engineering has been pivotal in the remarkable progress made in high-resolution imaging in the last decades. However, the diversity in PSF structures attainable through existing engineering methods is limited.…

Optics · Physics 2025-06-10 Md Sadman Sakib Rahman , Aydogan Ozcan

Three-dimensional spatiotemporal tracking of microscopic particles in multiple colors is a challenging optical imaging task. Existing approaches require a trade-off between photon-efficiency, field of view, mechanical complexity, spectral…

The knowledge of the exact structure of the optical system PSF enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications.…

Point-spread-function (PSF) engineering is a powerful computational imaging techniques wherein a custom phase mask is integrated into an optical system to encode additional information into captured images. Used in combination with deep…

Computer Vision and Pattern Recognition · Computer Science 2023-03-31 Sachin Shah , Sakshum Kulshrestha , Christopher A. Metzler

An approach reported recently by Alexandrov et al. on optical scatter imaging, termed digital Fourier microscopy (DFM), represents an adaptation of digital Fourier holography to selective imaging of biological matter. Holographic mode of…

Optics · Physics 2007-05-23 K. Y. T. Seet , P. Blazkiewicz , P. Meredith , A. V. Zvyagin

Modern design of complex optical systems relies heavily on computational tools. These typically utilize geometrical optics as well as Fourier optics, which enables the use of diffractive elements to manipulate light with features on the…

Optics · Physics 2022-07-27 Boris Ferdman , Alon Saguy , Onit Alalouf , Yoav Shechtman

We have developed a lab work module where we teach undergraduate students how to quantify the dynamics of a suspension of microscopic particles, measuring and analyzing the motion of those particles at the individual level or as a group.…

Soft Condensed Matter · Physics 2016-02-23 David Germain , Mathieu Leocmach , Thomas Gibaud

This review summarizes recent progress in investigating polymer systems by using Differential dynamic microscopy (DDM), a rapidly emerging approach that transforms a commercial microscope by combining real-space information with the…

Soft Condensed Matter · Physics 2021-06-07 Roberto Cerbino , Fabio Giavazzi , Matthew E. Helgeson

Within the expansive domain of optical sciences, achieving the precise characterization of light beams stands as a fundamental pursuit, pivotal for various applications, including telecommunications and imaging technologies. This study…

Point-spread-function (PSF) engineering is a well-established computational imaging technique that uses phase masks and other optical elements to embed extra information (e.g., depth) into the images captured by conventional CMOS image…

Computer Vision and Pattern Recognition · Computer Science 2024-06-14 Sachin Shah , Matthew Albert Chan , Haoming Cai , Jingxi Chen , Sakshum Kulshrestha , Chahat Deep Singh , Yiannis Aloimonos , Christopher Metzler

Propagation of an ultrashort pulse of light through strongly scattering media generates an intricate spatio-spectral speckle that can be described by means of the multi-spectral transmission matrix (MSTM). In conjunction with a spatial…

Recently introduced angular-memory-effect based techniques enable non-invasive imaging of objects hidden behind thin scattering layers. However, both the speckle-correlation and the bispectrum analysis are based on the statistical average…

Optics · Physics 2017-11-22 Tengfei Wu , Jonathan Dong , Xiaopeng Shao , Sylvain Gigan

A long-standing challenge in multiple-particle-tracking is the accurate and precise 3D localization of individual particles at close proximity. One established approach for snapshot 3D imaging is point-spread-function (PSF) engineering, in…

Image and Video Processing · Electrical Eng. & Systems 2020-10-01 Elias Nehme , Boris Ferdman , Lucien E. Weiss , Tal Naor , Daniel Freedman , Tomer Michaeli , Yoav Shechtman

Discrete delta functions define the limits of attainable spatial resolution for all imaging systems. Here we construct broad, multi-dimensional discrete functions that replicate closely the action of a Dirac delta function under aperiodic…

Image and Video Processing · Electrical Eng. & Systems 2020-09-01 I. D. Svalbe , D. M. Paganin , T. C. Petersen

This Point spread function (PSF) plays a crucial role in many computational imaging applications, such as shape from focus/defocus, depth estimation, and fluorescence microscopy. However, the mathematical model of the defocus process is…

Computer Vision and Pattern Recognition · Computer Science 2022-09-20 Renzhi He , Yan Zhuang , Boya Fu , Fei Liu

Spatial variables can be observed in many different forms, such as regularly sampled random fields (lattice data), point processes, and randomly sampled spatial processes. Joint analysis of such collections of observations is clearly…

Methodology · Statistics 2026-05-20 Jake P. Grainger , Tuomas A. Rajala , David J. Murrell , Sofia C. Olhede

We report a method to design at will the spatial profile of transmitted coherent light after propagation through a scattering sample. We compute an operator based on the experimentally measured transmission matrix, obtained by numerically…

Differential Dynamic Microscopy (DDM) is the combination of optical microscopy to statistical analysis to obtain information about the dynamical behaviour of a variety of samples spanning from soft matter physics to biology. In DDM, the…

Computer Vision and Pattern Recognition · Computer Science 2020-12-11 M. Norouzisadeh , G. Cerchiari , F. Croccolo

Point spread function (PSF) engineering is vital for precisely controlling the focus of light in computational imaging, with applications in neural imaging, fluorescence microscopy, and biophotonics. The PSF is derived from the magnitude of…

Optics · Physics 2025-04-22 Aleksey Valouev

We demonstrate a high-speed method to image objects through a thin scattering medium and around a corner. The method employs a reference object of known shape to retrieve the speckle-like point spread function of the scatterer. We extract…

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