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Cryo-electron microscopy (cryo-EM) is a powerful technique for determining the structure of proteins and other macromolecular complexes at near-atomic resolution. In single particle cryo-EM, the central problem is to reconstruct the…

Quantitative Methods · Quantitative Biology 2020-02-18 Ellen D. Zhong , Tristan Bepler , Joseph H. Davis , Bonnie Berger

Automated and semi-automated techniques in biomedical electron microscopy (EM) enable the acquisition of large datasets at a high rate. Segmentation methods are therefore essential to analyze and interpret these large volumes of data, which…

Computer Vision and Pattern Recognition · Computer Science 2023-08-08 Anusha Aswath , Ahmad Alsahaf , Ben N. G. Giepmans , George Azzopardi

We consider the problem of accurately identifying cell boundaries and labeling individual cells in confocal microscopy images, specifically, 3D image stacks of cells with tagged cell membranes. Precise identification of cell boundaries,…

Computer Vision and Pattern Recognition · Computer Science 2019-09-06 Jiaxiang Jiang , Po-Yu Kao , Samuel A. Belteton , Daniel B. Szymanski , B. S. Manjunath

Cryo-Electron Microscopy (cryo-EM) has emerged as a key technology to determine the structure of proteins, particularly large protein complexes and assemblies in recent years. A key challenge in cryo-EM data analysis is to automatically…

Biomolecules · Quantitative Biology 2023-04-04 Nabin Giri , Raj S. Roy , Jianlin Cheng

Cryogenic electron microscopy (cryo-EM) has transformed structural biology by allowing to reconstruct 3D biomolecular structures up to near-atomic resolution. However, the 3D reconstruction process remains challenging, as the 3D structures…

Computer Vision and Pattern Recognition · Computer Science 2023-07-18 Bongjin Koo , Julien Martel , Ariana Peck , Axel Levy , Frédéric Poitevin , Nina Miolane

Cellular Electron Cryotomography (CryoET) offers the ability to look inside cells and observe macromolecules frozen in action. A primary challenge for this technique is identifying and extracting the molecular components within the crowded…

Quantitative Methods · Quantitative Biology 2021-08-04 Muyuan Chen , Wei Dai , Ying Sun , Darius Jonasch , Cynthia Y He , Michael F. Schmid , Wah Chiu , Steven J Ludtke

In this work we set out to find a method to classify protein structures using a Deep Learning methodology. Our Artificial Intelligence has been trained to recognize complex biomolecule structures extrapolated from the Protein Data Bank…

Machine Learning · Computer Science 2021-11-04 Damiano Perri , Marco Simonetti , Andrea Lombardi , Noelia Faginas-Lago , Osvaldo Gervasi

Motivation: Structural heterogeneity in single-particle cryo-electron microscopy (cryo-EM) data represents a major challenge for high-resolution structure determination. Unsupervised classification may serve as the first step in the…

Data Analysis, Statistics and Probability · Physics 2017-11-01 Jiayi Wu , Yong-Bei Ma , Charles Congdon , Bevin Brett , Shuobing Chen , Qi Ouyang , Youdong Mao

Cryo-Electron Microscopy (Cryo-EM) is a Nobel prize-winning technology for determining the 3D structure of particles at near-atomic resolution. A fundamental step in the recovering of the 3D single-particle structure is to align its 2D…

Image and Video Processing · Electrical Eng. & Systems 2021-01-12 Koby Bibas , Gili Weiss-Dicker , Dana Cohen , Noa Cahan , Hayit Greenspan

The advancement of the neuroscientific imaging techniques has produced an unprecedented size of neural cell imaging data, which calls for automated processing. In particular, identification of cells from two photon images demands…

Image and Video Processing · Electrical Eng. & Systems 2019-09-26 Si-Baek Seong , Hae-Jeong Park

Tracking cells in 3D at high speed continues to attract extensive attention for many biomedical applications, such as monitoring immune cell migration and observing tumor metastasis in flowing blood vessels. Here, we propose a deep…

Optics · Physics 2018-05-16 Kan Liu , Hui Qiao , Jiamin Wu , Haoqian Wang , Lu Fang , Qionghai Dai

This paper presents a novel deep learning architecture to classify structured objects in datasets with a large number of visually similar categories. We model sequences of images as linear-chain CRFs, and jointly learn the parameters from…

Computer Vision and Pattern Recognition · Computer Science 2019-11-19 Eran Goldman , Jacob Goldberger

Accurate prediction of recurrence in clear cell renal cell carcinoma (ccRCC) remains a major clinical challenge due to the disease complex molecular, pathological, and clinical heterogeneity. Traditional prognostic models, which rely on…

Image and Video Processing · Electrical Eng. & Systems 2025-07-11 Hasaan Maqsood , Saif Ur Rehman Khan

Inferring biological relationships from cellular phenotypes in high-content microscopy screens provides significant opportunity and challenge in biological research. Prior results have shown that deep vision models can capture biological…

Uncovering the heterogeneity of cell populations is a long-standing goal in fields ranging from antimicrobial resistance to cancer research. Emerging technology platforms such as droplet microfluidics hold the promise to decipher cellular…

Image and Video Processing · Electrical Eng. & Systems 2020-01-29 Vasileios Anagnostidis , Benjamin Sherlock , Jeremy Metz , Philip Mair , Florian Hollfelder , Fabrice Gielen

Single particle cryo-electron microscopy (EM) is an increasingly popular method for determining the 3-D structure of macromolecules from noisy 2-D images of single macromolecules whose orientations and positions are random and unknown. One…

Computer Vision and Pattern Recognition · Computer Science 2017-04-11 Roy R. Lederman , Amit Singer

Single-particle cryo-electron microscopy (cryo-EM) has recently joined X-ray crystallography and NMR spectroscopy as a high-resolution structural method to resolve biological macromolecules. In a cryo-EM experiment, the microscope produces…

Information Theory · Computer Science 2022-10-28 Tamir Bendory , Nicolas Boumal , William Leeb , Eitan Levin , Amit Singer

Automatic cell tracking in dense environments is plagued by inaccurate correspondences and misidentification of parent-offspring relationships. In this paper, we introduce a novel cell tracking algorithm named DenseTrack, which integrates…

Image and Video Processing · Electrical Eng. & Systems 2024-07-08 Tanjin Taher Toma , Yibo Wang , Andreas Gahlmann , Scott T. Acton

Cryo-electron microscopy (cryo-EM) has revolutionized structural biology by enabling near-atomic-level visualization of biomolecular assemblies. However, the exponential growth in cryo-EM data throughput and complexity, coupled with diverse…

Quantitative Methods · Quantitative Biology 2026-02-25 Weining Fu , Kai Shu , Kui Xu , Qiangfeng Cliff Zhang

Automated methods for breast cancer detection have focused on 2D mammography and have largely ignored 3D digital breast tomosynthesis (DBT), which is frequently used in clinical practice. The two key challenges in developing automated…

Computer Vision and Pattern Recognition · Computer Science 2020-02-28 Yu Zhang , Xiaoqin Wang , Hunter Blanton , Gongbo Liang , Xin Xing , Nathan Jacobs