Related papers: 3D correlative single-cell imaging utilizing fluor…
Biological tissues are composed of discrete compartments of biochemical media that often exhibit subtle differences in refractive index. Light propagating through these compartments partially diffracts in a forward direction with a pi/2…
Diffraction tomography is an inverse scattering technique used to reconstruct the spatial distribution of the material properties of a weakly scattering object. The object is exposed to radiation, typically light or ultrasound, and the…
Single Image Reflection Removal (SIRR) technique plays a crucial role in image processing by eliminating unwanted reflections from the background. These reflections, often caused by photographs taken through glass surfaces, can…
This paper presents a new FDTD-based optical simulation model dedicated to describe the optical performances of CMOS image sensors taking into account diffraction effects. Following market trend and industrialization constraints, CMOS image…
Using Fermat's least optical path principle the family of ray-trajectories through a special but common type of a gradient refractive index lens, n(r)=n_0+\Delta n R/r, is solved analytically. The solution, i.e. the ray-equation r(phi), is…
Optical Coherence Tomography(OCT) is a non-invasive technique capturing cross-sectional area of the retina in micro-meter resolutions. It has been widely used as a auxiliary imaging reference to detect eye-related pathology and predict…
In this chapter, we introduce digital holographic microscopy (DHM) as a marker-free method to determine the refractive index of single, spherical cells in suspension. The refractive index is a conclusive measure in a biological context.…
Optical Coherence Tomography (OCT) is the primary imaging modality for detecting pathological biomarkers associated to retinal diseases such as Age-Related Macular Degeneration. In practice, clinical diagnosis and treatment strategies are…
Optical coherence tomography (OCT) is a micrometer-scale, volumetric imaging modality that has become a clinical standard in ophthalmology. OCT instruments image by raster-scanning a focused light spot across the retina, acquiring…
Three-dimensional (3D) imaging of the subcellular organisation and morphology of cells and tissues is essential for understanding biological function. Although staining is the most widely used approach for visualising biological samples…
Visible photonic integrated circuits underpin applications ranging from AR/VR to quantum control, yet lack a high-resolution, nondestructive diagnostic comparable to the optical frequency-domain reflectometry used in infrared silicon…
An organoid is a three-dimensional (3D) in vitro cell culture emulating human organs. We applied 3D dynamic optical coherence tomography (DOCT) to visualize the intratissue and intracellular activities of human induced pluripotent stem…
We report a proof-of-concept demonstration of a tunable infrared (IR) optical coherence tomography (OCT) technique with detection of only visible range photons. Our method is based on the nonclassical interference of frequency correlated…
In optical imaging, light propagation is affected by the inhomogeneities of the medium. Sample-induced aberrations and multiple scattering can strongly degrade the image resolution and contrast. Based on a dynamic correction of the incident…
Fluorescent nuclear track detectors (FNTDs) based on Al2O3:C,Mg single crystal combined with confocal microscopy provide 3D information on ion tracks with a resolution only limited by light diffraction. FNTDs are also ideal substrates to be…
Fluorescent observation of cells generally suffers from the limited axial resolution due to the elongated point spread function of the microscope optics. Consequently, three-dimensional imaging results in axial resolution being several…
An approach reported recently by Alexandrov et al. on optical scatter imaging, termed digital Fourier microscopy (DFM), represents an adaptation of digital Fourier holography to selective imaging of biological matter. Holographic mode of…
By switching fluorophores on and off in either a deterministic or a stochastic manner, superresolution microscopy has enabled the imaging of biological structures at resolutions well beyond the diffraction limit. Superresolution optical…
Optical diffraction tomography measures the three-dimensional refractive index map of a specimen and visualizes biochemical phenomena at the nanoscale in a non-destructive manner. One major drawback of optical diffraction tomography is poor…
By 2025, holotomography (HT) has matured from a niche optical modality into a versatile platform for quantitative, label-free imaging in biomedicine. By reconstructing the three-dimensional refractive-index (RI) distribution of cells and…