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Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…

Diffuse correlation spectroscopy (DCS) is a powerful tool for assessing microvascular hemodynamic in deep tissues. Recent advances in sensors, lasers, and deep learning have further boosted the development of new DCS methods. However,…

A fluorescent image analysis procedure to determine the distribution of species concentration and density in a gas flow is proposed. The fluorescent emission is due to the excitation of atoms/molecules of a gas that is intercepted by an…

Fluid Dynamics · Physics 2009-11-13 Marco Belan , Sergio De Ponte , Daniela Tordella

Recent experimental results have shown that active enzymes can diffuse faster when they are in the presence of their substrates. Fluorescence correlation spectroscopy (FCS), which relies on analyzing the fluctuations in fluorescence…

Subcellular Processes · Quantitative Biology 2019-09-25 Mengqi Xu , Lyanne Valdez , Aysuman Sen , Jennifer L. Ross

The Fluorescence Correlation Spectroscopy (FCS) spectrum G(t) and Raster Image Correlation Spectroscopy (RICS) spectrum R(t) of dilute diffusing particles are determined by the displacement distribution function P(x,t) of the particles and…

Soft Condensed Matter · Physics 2017-06-07 George D. J. Phillies

We present an efficient approach and principle experiment for compressive sensing (CS) fluorescence spectral imaging. According to the dimension-reduced effect of CS, the spectral and spatial information was simultaneously obtained by using…

Instrumentation and Detectors · Physics 2017-07-07 Chao Wang , Xue-Feng Liu , Wen-Kai Yu , Xu-Ri Yao , Fu Zheng , Qian Dong , Ruo-Ming Lan , Guang-Jie Zhai , Qing Zhao

Aberrations and multiple scattering in biological tissues critically distort light beams into highly complex speckle patterns. In this regard, digital optical phase conjugation (DOPC) is a promising technique enabling in-depth focusing.…

Imaging techniques such as functional near-infrared spectroscopy (fNIRS) and diffuse optical tomography (DOT) achieve deep, non-invasive sensing in turbid media, but they are constrained by the photon budget. Wavefront shaping (WFS) can…

Optics · Physics 2026-04-21 Pablo Jara , Arthur Goetschy , Hui Cao , Alexey Yamilov

A multispeckle technique for efficiently measuring correctly ensemble-averaged intensity autocorrelation functions of scattered light from non-ergodic and/or non-stationary systems is described. The method employs a CCD camera as a…

Soft Condensed Matter · Physics 2009-11-07 Virgile. Viasnoff , Francois Lequeux , D. J. Pine

We propose and demonstrate a novel technique that combines Raman scattering and optical cycling in molecules with diagonal Franck-Condon factors. This resonance Raman optical cycling manipulates molecules to behave like efficient…

Atomic Physics · Physics 2021-08-26 J. C. Shaw , J. C. Schnaubelt , D. J. McCarron

The inherent non-linearity of intensity correlation functions can be used to spatially distinguish identical emitters beyond the diffraction limit, as achieved, for example, in Super-Resolution Optical Fluctuation Imaging (SOFI). Here, we…

Optics · Physics 2024-12-18 Yifan Chen , Chieh Tsao , Hendrik Utzat

In this article we present a new diagnostic approach utilizing flow-cytometry to study compounds of nanoparticle samples in solution by analysis of their scattering patterns retrieved from the cytometric measurements. As a specific…

The diffusion of macromolecules in cells and in complex fluids is often found to deviate from simple Fickian diffusion. One explanation offered for this behavior is that molecular crowding renders diffusion anomalous, where the mean-squared…

Soft Condensed Matter · Physics 2016-05-18 Daniel S. Banks , Charmaine Tressler , Robert D. Peters , Felix Höfling , Cécile Fradin

Background-free fluorescence detection in multidimensional electronic spectroscopy promises high sensitivity compared to conventional approaches. Here we explore the sensitivity limits of multidimensional electronic spectroscopy. We present…

Chemical Physics · Physics 2022-10-18 Amitav Sahu , Vivek N. Bhat , Sanjoy Patra , Vivek Tiwari

Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…

Optics · Physics 2026-03-30 Lei Zhu , Tengfei Wu , Bernhard Rauer , Hilton B. de Aguiar , Sylvain Gigan

Fluorescence spectroscopy and modeling provide powerful means to characterize biomacromolecular structures, dynamics, and interactions. F\"orster resonance energy transfer serves as a key technique for this due to its nanometer-scale…

Biological Physics · Physics 2026-02-26 Thomas-Otavio Peulen , Daria Maksutova , Thorben Cordes

In this article we derive the average and the variance of the cross-correlation of a noise wavefield. The noise cross-correlation function (NCF) is widely used to passively estimate the Green's function between two probes and is…

Disordered Systems and Neural Networks · Physics 2015-06-17 Julien de Rosny , Matthieu Davy

The investigation of the static and dynamic structural properties of colloidal systems relies on techniques capable of atomic resolution in real space and femtosecond resolution in time. Recently, the cross-correlation function (CCF)…

Other Condensed Matter · Physics 2015-11-20 Tatiana Latychevskaia , Giulia Fulvia Mancini , Fabrizio Carbone

Single-molecule sensitive microscopies and spectroscopies are transforming biophysics and materials science laboratories. Techniques such as fluorescence correlation spectroscopy (FCS) and single-molecule sensitive fluorescence resonance…

Event-based image sensors provide microsecond temporal resolution but lack spectral discrimination, whereas diffractive spectral imagers encode wavelength information at conventional frame rates. We introduce a fluorescence microscopy…

Optics · Physics 2026-03-20 Richard G. Baird , Boyden Myers , Erik M. Jorgensen , Rajesh Menon