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Related papers: Domain Boundary Detection in Hi-C Maps: A Probabil…

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Modern biological techniques such as Hi-C permit to measure probabilities that different chromosomal regions are close in space. These probabilities can be visualised as matrices called contact maps. In this paper, we introduce a…

Biomolecules · Quantitative Biology 2020-10-21 Simone Pigolotti , Mogens H. Jensen , Yinxiu Zhan , Guido Tiana

To better understand DNA's 3D folding in cell nuclei, researchers developed chromosome capture methods such as Hi-C that measure the contact frequencies between all DNA segment pairs across the genome. As Hi-C data sets often are massive,…

Biological Physics · Physics 2023-11-14 Anton Holmgren , Dolores Bernenko , Ludvig Lizana

Chromosome conformation capture experiments such as Hi-C are used to map the three-dimensional spatial organization of genomes. One specific feature of the 3D organization is known as topologically associating domains (TADs), which are…

Applications · Statistics 2019-10-18 Y. X. Rachel Wang , Purnamrita Sarkar , Oana Ursu , Anshul Kundaje , Peter J. Bickel

The probability of two loci, separated by a certain genome length, being in contact can be inferred using the Chromosome Conformation Capture (3C) method and related Hi-C experiments. How to go from the contact map, a matrix listing the…

Soft Condensed Matter · Physics 2021-03-24 Guang Shi , D. Thirumalai

Motivation: Hi-C matrices are cornerstones for qualitative and quantitative studies of genome folding, from its territorial organization to compartments and topological domains. The high dynamic range of genomic distances probed in Hi-C…

Genomics · Quantitative Biology 2021-01-27 Stefano Franzini , Marco Di Stefano , Cristian Micheletti

Combining genome-wide structural models with phenomenological data is at the forefront of efforts to understand the organizational principles regulating the human genome. Here, we use chromosome-chromosome contact data as knowledge-based…

Biological Physics · Physics 2016-10-31 Marco Di Stefano , Jonas Paulsen , Tonje G. Lien , Eivind Hovig , Cristian Micheletti

Researchers developed chromosome capture methods such as Hi-C to better understand DNA's 3D folding in nuclei. The Hi-C method captures contact frequencies between DNA segment pairs across the genome. When analyzing Hi-C data sets, it is…

Physics and Society · Physics 2023-11-14 Dolores Bernenko , Sang Hoon Lee , Ludvig Lizana

The statistical properties of intra-chromosome maps obtained by a genome-wide chromosome conformation capture method (Hi-C) are described in the framework of the hierarchical crumpling model of heteropolymer chain with quenched disorder in…

Genomics · Quantitative Biology 2013-12-02 L. Nazarov , M. V. Tamm , S. K. Nechaev , V. A. Avetisov

Several experiments show that the three dimensional (3D) organization of chromosomes affects genetic processes such as transcription and gene regulation. To better understand this connection, researchers developed the Hi-C method that is…

Genomics · Quantitative Biology 2019-05-03 Sang Hoon Lee , Yeonghoon Kim , Sungmin Lee , Xavier Durang , Per Stenberg , Jae-Hyung Jeon , Ludvig Lizana

One of the most important tasks in understanding the complex spatial organization of the genome consists in extracting information about this spatial organization, the function and structure of chromatin topological domains from existing…

Biological Physics · Physics 2016-01-07 Alexander P. Moscalets , Leonid I. Nazarov , Mikhail V. Tamm

Just as in eukaryotes, high-throughput chromosome conformation capture (Hi-C) data have revealed nested organizations of bacterial chromosomes into overlapping interaction domains. In this chapter, we present a multiscale analysis framework…

Genomics · Quantitative Biology 2020-10-06 Nelle Varoquaux , Virginia S. Lioy , Frédéric Boccard , Ivan Junier

Community detection (CD) algorithms are applied to Hi-C data to discover new communities of loci in the 3D conformation of human and mouse DNA. We find that CD has some distinct advantages over pre-existing methods: (1) it is capable of…

Genomics · Quantitative Biology 2015-09-18 Irineo Cabreros , Emmanuel Abbe , Aristotelis Tsirigos

Chromatin is a polymer complex of DNA and proteins that regulates gene expression. The three-dimensional structure and organization of chromatin controls DNA transcription and replication. High-throughput chromatin conformation capture…

Structural variants compose the majority of human genetic variation, but are difficult to assess using current genomic sequencing technologies. Optical mapping technologies, which measure the size of chromosomal fragments between labeled…

Quantitative Methods · Quantitative Biology 2019-10-10 Weiwei Li , Jan Hannig , Corbin Jones

In this article, we show how the recent statistical techniques developed in Topological Data Analysis for the Mapper algorithm can be extended and leveraged to formally define and statistically quantify the presence of topological…

Computational Geometry · Computer Science 2018-12-05 Mathieu Carriere , Raul Rabadan

Hi-C sequencing is widely used for analyzing chromosomal interactions. In this study, we propose "superimposed Hi-C" which features paired EcoP15I sites in a linker to facilitate sticky-end ligation with target DNAs. Superimposed Hi-C…

Genomics · Quantitative Biology 2024-08-26 Jia Zhang , Li Xiao , Peng Qi , Yaling Zeng , Xumeng Chen , Duan-fang Liao , Kai Li

Chromosome capture techniques like Hi-C have expanded our understanding of mammalian genome 3D architecture and how it influences gene activity. To analyze Hi-C data sets, researchers increasingly treat them as DNA-contact networks and use…

Biological Physics · Physics 2024-05-10 Lucas Hedström , Antón Carcedo Martínez , Ludvig Lizana

Biological processes underlying the basic functions of a cell involve complex interactions between genes. From a technical point of view, these interactions can be represented through a graph where genes and their connections are,…

Methodology · Statistics 2020-11-24 Thi Kim Hue Nguyen , Monica Chiogna

Hi-C experiments are used to infer the contact probabilities between loci separated by varying genome lengths. Contact probability should decrease as the spatial distance between two loci increases. However, studies comparing Hi-C and FISH…

Soft Condensed Matter · Physics 2020-05-25 Guang Shi , D. Thirumalai

Recent chromosome conformation capture experiments have led to the discovery of dense, contiguous, megabase-sized topological domains that are similar across cell types and conserved across species. These domains are strongly correlated…

Quantitative Methods · Quantitative Biology 2013-07-31 Darya Filippova , Rob Patro , Geet Duggal , Carl Kingsford
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