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Complex biochemical pathways or regulatory enzyme kinetics can be reduced to chains of elementary reactions, which can be described in terms of chemical kinetics. This discipline provides a set of tools for quantifying and understanding the…

Quantitative Methods · Quantitative Biology 2018-01-08 Elena Agliari , Adriano Barra , Giulio Landolfi , Sara Murciano , Sarah Perrone

Genetically identical cells under the same environmental conditions can show strong variations in protein copy numbers due to inherently stochastic events in individual cells. We here develop a theoretical framework to address how…

Subcellular Processes · Quantitative Biology 2017-08-23 Pan-Jun Kim , Nathan D. Price

We study a class of Stochastic Differential Equations (SDEs) with jumps modeling multistage Michaelis--Menten enzyme kinetics, in which a substrate is sequentially transformed into a product via a cascade of intermediate complexes. These…

Probability · Mathematics 2026-04-14 Arnab Ganguly , Wasiur R. KhudaBukhsh

We study the effect of restart, and retry, on the mean completion time of a generic process. The need to do so arises in various branches of the sciences and we show that it can naturally be addressed by taking advantage of the classical…

Statistical Mechanics · Physics 2020-10-30 Tal Rotbart , Shlomi Reuveni , Michael Urbakh

A different view of Henri-Michaelis-Menten (HMM) enzyme kinetics is presented. In the first part of the paper, a simplified but useful description that stresses the cyclic nature of the catalytic process is introduced. The time-dependence…

Chemical Physics · Physics 2009-05-07 Mario N. Berberan-Santos

This paper studies a mathematical formalism of nonequilibrium thermodynamics for chemical reaction models with $N$ species, $M$ reactions, and general rate law. We establish a mathematical basis for J. W. Gibbs' macroscopic chemical…

Mathematical Physics · Physics 2017-01-04 Hao Ge , Hong Qian

Reaction constants in traditional Michaelis-Menten type enzyme kinetics are most often determined through a linear Lineweaver-Burk plot. While such a graphical plot is sometimes good to achieve the end, it is always better to go for a few…

Chemical Physics · Physics 2017-10-24 Sharmistha Dhatt , Kamal Bhattacharyya

Classical descriptions of enzyme kinetics ignore the physical nature of the intracellular environment. Main implicit assumptions behind such approaches are that reactions occur in compartment volumes which are large enough so that molecular…

Subcellular Processes · Quantitative Biology 2009-10-26 Ramon Grima

In canonical models of Micro-Electro Mechanical Systems (MEMS), an event called touch- down whereby the electrical components of the device come into contact, is characterized by a blow up in the governing equations and a non-physical…

Analysis of PDEs · Mathematics 2013-10-02 A. E. Lindsay , J. Lega , K. B. Glasner

Biomolecular condensates in cells are often rich in catalytically-active enzymes. This is particularly true in the case of the large enzymatic complexes known as metabolons, which contain different enzymes that participate in the same…

Subcellular Processes · Quantitative Biology 2021-10-20 Vincent Ouazan-Reboul , Jaime Agudo-Canalejo , Ramin Golestanian

We study chemical reactions with complex mechanisms under two assumptions: (i) intermediates are present in small amounts (this is the quasi-steady-state hypothesis or QSS) and (ii) they are in equilibrium relations with substrates (this is…

Chemical Physics · Physics 2014-04-15 A. N. Gorban , M. Shahzad

Kinetic Monte Carlo methods such as the Gillespie algorithm model chemical reactions as random walks in particle number space. The inter-reaction times are exponentially distributed under the assumption that the system is well mixed. We…

Statistical Mechanics · Physics 2018-01-17 Tomás Aquino , Marco Dentz

We examine the stochastic dynamics of two enzymes that are mechanically coupled to each other, e.g., through an elastic substrate or a fluid medium. The enzymes undergo conformational changes during their catalytic cycle, which itself is…

Statistical Mechanics · Physics 2021-11-16 Jaime Agudo-Canalejo , Tunrayo Adeleke-Larodo , Pierre Illien , Ramin Golestanian

Quasi-steady state reductions for the irreversible Michaelis--Menten reaction mechanism are of interest both from a theoretical and an experimental design perspective. A number of publications have been devoted to extending the parameter…

Chemical Physics · Physics 2023-03-21 Justin Eilertsen , Santiago Schnell , Sebastian Walcher

In this paper we study noise-induced bistability in a specific circuit with many biological implications, namely a single-step enzymatic cycle described by Michaelis Menten equations with quasi-steady state assumption. We study the system…

Biological Physics · Physics 2011-07-25 Daniel Remondini , Enrico Giampieri , Armando Bazzani , Gastone Castellani , Amos Maritan

This paper presents the investigation of convolutional neural network (CNN) prediction successfully recognizing the temperature of the non-equilibrium phases and phase transitions in two-dimensional (2D) Ising spins on square-lattice. The…

Statistical Mechanics · Physics 2024-05-07 Dagne Wordofa , Mulugeta Bekele

The sequence of amino acid monomers in the primary structure of a protein is decided by the corresponding sequence of codons (triplets of nucleic acid monomers) on the template messenger RNA (mRNA). The polymerization of a protein, by…

Biological Physics · Physics 2017-06-02 Annwesha Dutta , Debashish Chowdhury

Binding and unbinding of ligands to specific sites of a macromolecule are one of the most elementary molecular interactions inside the cell that embody the computational processes of biological regulations. The interaction between…

Molecular Networks · Quantitative Biology 2011-11-10 Hidenori Kimura , Hiroyuki Okano , Reiko J. Tanaka

Cognitive diagnosis is a crucial task in computational education, aimed at evaluating students' proficiency levels across various knowledge concepts through exercises. Current models, however, primarily rely on students' answered exercises,…

Computers and Society · Computer Science 2023-12-19 Haiping Ma , Changqian Wang , Hengshu Zhu , Shangshang Yang , Xiaoming Zhang , Xingyi Zhang

A minimal design for a molecular swimmer is proposed that is a based on a mechanochemical propulsion mechanism. Conformational changes are induced by electrostatic actuation when specific parts of the molecule temporarily acquire net…

Biological Physics · Physics 2015-05-19 Ramin Golestanian