Related papers: Light Microscopy at Maximal Precision
Real-world imaging systems acquire measurements that are degraded by noise, optical aberrations, and other imperfections that make image processing for human viewing and higher-level perception tasks challenging. Conventional cameras…
Fourier ptychography microscopy (FPM) is a new computational imaging technique that can provide gigapixel images with both high resolution and a wide field of view (FOV). However, time consuming of the data-acquisition process is a critical…
We present an algorithm for performing precise aperture photometry on critically sampled astrophysical images. The method is intended to overcome the small-aperture limitations imposed by point-sampling. Aperture fluxes are numerically…
In the conventional approach to lens imaging, rays are used to map object points to image points. However, many students have a need to think of the image as a whole. To answer this need, lens imaging is reinterpreted as a superposition of…
A simple method of constructing the 3D surface of non-transparent micro-objects by extending the depth-of-field on the whole attainable surface is presented. The series of images of a sample are recorded by the sequential movement of the…
Single-particle cryo-electron microscopy (cryo-EM) has recently joined X-ray crystallography and NMR spectroscopy as a high-resolution structural method to resolve biological macromolecules. In a cryo-EM experiment, the microscope produces…
Optical images of transparent three-dimensional objects can be different from a replica of the object's cross section in the image plane due to refraction at the surface or in the body of the object. Simulations of the object's image are…
Infrared (IR) imaging has become a viable tool for visualizing various chemical bonds in a specimen. The performance, however, is limited in terms of spatial resolution and imaging speed. Here, instead of measuring the loss of the IR beam,…
We present a fast and accurate method for dense depth reconstruction from sparsely sampled light fields obtained using a synchronized camera array. In our method, the source images are over-segmented into non-overlapping compact superpixels…
Aberration-corrected optics have made electron microscopy at atomic-resolution a widespread and often essential tool for nanocharacterization. Image resolution is dominated by beam energy and the numerical aperture of the lens ({\alpha}),…
The transmission electron microscope facilitates the highest-resolution imaging of any instrument ever created, and its limiting factor is no longer spatial resolution but dose efficiency. Low electron doses avoid sample damage but produce…
Feynman once asked physicists to build better electron microscopes to be able to watch biology at work. While electron microscopes can now provide atomic resolution, electron beam induced specimen damage precludes high resolution imaging of…
Quality control in molecular optical sectioning microscopy is indispensable for transforming acquired digital images from qualitative descriptions to quantitative data. Although numerous tools, metrics, and phantoms have been developed,…
Quantitative phase imaging (QPI) is important in many applications such as microscopy and crystallography. To quantitatively reveal phase information, people could either employ interference to map phase distribution into intensity fringes,…
Spectroscopic photoemission microscopy is a well-established method to investigate the electronic structure of surfaces. In modern photoemission microscopes the electron optics allows imaging of the image plane, momentum plane, or…
An optical microscope enables image-based findings and diagnosis on microscopic targets, which is indispensable in many scientific, industrial and medical settings. Majority of microscope users are accustomed to standard benchtop…
Light microscopy as well as image acquisition and processing suffer from physical and technical prejudices which preclude a correct interpretation of biological observations which can be reflected in, e.g., medical and pharmacological…
Hyperspectroscopy is a new method of surface image taking, providing simultaneously high position and spectral resolutions which allow one to make some conclusions about chemical compositions of the surfaces. We are now studying…
Low-light optical imaging refers to the use of cameras to capture images with minimal photon flux. This area has broad application to diverse fields, including optical microscopy for biological studies. In such studies, it is important to…
The process of acquiring microscopic images in life sciences often results in image degradation and corruption, characterised by the presence of noise and blur, which poses significant challenges in accurately analysing and interpreting the…