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Related papers: Light Microscopy at Maximal Precision

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Over the last decade, the light microscope has become increasingly useful as a quantitative tool for studying colloidal systems. The ability to obtain particle coordinates in bulk samples from micrographs is particularly appealing. In this…

Soft Condensed Matter · Physics 2007-09-27 Matthew C. Jenkins , Stefan U. Egelhaaf

We propose a novel technique of microscopy to overcome the effects of both scattering and limitation of the accessible depth due to the objective working distance. By combining Laser Optical Feedback Imaging (LOFI) with Acoustic Photon…

Light microscopy allows observing cellular features and objects with sub-micrometer resolution. As such, light microscopy has been playing a fundamental role in the life sciences for more than a hundred years. Fueled by the availability of…

Other Quantitative Biology · Quantitative Biology 2021-10-28 Johannes Hohlbein , Benedict Diederich , Barbora Marsikova , Emmanuel G. Reynaud , Seamus Holden , Wiebke Jahr , Robert Haase , Kirti Prakash

The most realistic information about the transparent sample such as a live cell can be obtained only using bright-field light microscopy. At high-intensity pulsing LED illumination, we captured a primary 12-bit-per-channel (bpc) response…

Image and Video Processing · Electrical Eng. & Systems 2021-08-18 Ganna Platonova , Dalibor Stys , Pavel Soucek , Kirill Lonhus , Jan Valenta , Renata Rychtarikova

Hyperspectral microscopy is an imaging technique that provides spectroscopic information with high spatial resolution. When applied in the relevant wavelength region, such as in the infrared (IR), it can reveal a rich spectral fingerprint…

Optical stellar interferometers have demonstrated milli-arcsecond resolution with few apertures spaced hundreds of meters apart. To obtain rich direct images, many apertures will be needed, for a better sampling of the incoming wavefront.…

Instrumentation and Methods for Astrophysics · Physics 2014-09-17 Arun Surya , Swapan K. Saha , Antoine Labeyrie

Fourier Ptychographic Microscopy (FPM) is a computational imaging method that is able to super-resolve features beyond the diffraction-limit set by the objective lens of a traditional microscope. This is accomplished by using synthetic…

Signal Processing · Electrical Eng. & Systems 2019-04-09 Michael Kellman , Emrah Bostan , Michael Chen , Laura Waller

Although several optical techniques have been recently developed in order to overcome the resolution limit in microscopy, the imaging of sub-wavelength features is still a real challenge. In practise, super-resolution techniques remain…

Physics Education · Physics 2019-07-24 Stephane Perrin , Keshia Badu , Paul Montgomery , Sylvain Lecler

Owing to its capacity for unique (bio)-chemical specificity, microscopy withmid-IR illumination holds tremendous promise for a wide range of biomedical and industrial applications. The primary limitation, however, remains detection; with…

Conventional microscopic records represent intensity distributions whereby local sample information is mapped onto local information at the detector. In coherent microscopy, the superposition principle of waves holds; field amplitudes are…

Optics · Physics 2013-11-22 Tatiana Latychevskaia , Hans-Werner Fink

Label-free optical absorption microscopy techniques have evolved as effective tools for non-invasive chemical specific structural, and functional imaging. Yet most modern label-free microscopy modalities target only a fraction of the…

We present novel methods to perform plenoptic imaging at the diffraction limit by measuring intensity correlations of light. The first method is oriented towards plenoptic microscopy, a promising technique which allows refocusing and…

Investigation of cell structure is hardly imaginable without bright-field microscopy. Numerous modifications such as depth-wise scanning or videoenhancement make this method being state-of-the-art. This raises a question what maximal…

Image and Video Processing · Electrical Eng. & Systems 2020-10-29 Kirill Lonhus , Renata Rychtarikova , Ganna Platonova , Dalibor Stys

We present an overview of the performances of a plenoptic microscope which combines the high sensitivity of a laser optical feedback imaging setup , the high resolution of optical synthetic aperture and a shot noise limited signal to noise…

Optics · Physics 2015-06-19 W Glastre , O Hugon , O Jacquin , H Guillet de Chatellus , E Lacot

The extraordinary improvements of modern imaging devices offer access to data with unprecedented information content. However, widely used image processing methodologies fall far short of exploiting the full breadth of information offered…

Computer Vision and Pattern Recognition · Computer Science 2014-03-27 Benjamin Berkels , Peter Binev , Douglas A. Blom , Wolfgang Dahmen , Robert C. Sharpley , Thomas Vogt

Analysis of microscope images is a tedious work which requires patience and time, usually done manually by the microscopist after data collection. Here we introduce an approach of automatic image analysis, which is based on locally applied…

Image and Video Processing · Electrical Eng. & Systems 2019-12-17 Benedykt R. Jany , Arkadiusz Janas , Franciszek Krok

We demonstrate that a deep neural network can significantly improve optical microscopy, enhancing its spatial resolution over a large field-of-view and depth-of-field. After its training, the only input to this network is an image acquired…

Machine Learning · Computer Science 2017-11-21 Yair Rivenson , Zoltan Gorocs , Harun Gunaydin , Yibo Zhang , Hongda Wang , Aydogan Ozcan

Recently, imaging by microspheres and dielectric particle-lenses emerged as a simple solution to obtaining super-resolution images of nanoscale devices and structures. Calibrated resolution of ~{\lambda}/6 - {\lambda}/8 has been…

Optics · Physics 2021-08-26 Zengbo Wang , Boris Luk'yanchuk

Fourier ptychographic (FP) microscope is a coherent imaging method that can synthesize an image with a higher bandwidth using multiple low-bandwidth images captured at different spatial frequency regions. The method's demand for multiple…

Optics · Physics 2016-03-25 Jaebum Chung , Hangwen Lu , Xiaoze Ou , Haojiang Zhou , Changhuei Yang

Image processing and pattern recognition offer a useful and versatile method for optically characterizing drops of a colloidal solution during the drying process and in its final state. This paper exploits image processing techniques…

Soft Condensed Matter · Physics 2020-02-11 Anusuya Pal , Amalesh Gope , Germano S. Iannacchione
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