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Confocal microscopy, a critical advancement in optical imaging, is widely applied because of its excellent anti-noise ability. However, it has low imaging efficiency and can cause phototoxicity. Optical-sectioning structured illumination…

Optics · Physics 2024-05-27 Weishuai Zhou , Manhong Yao , Xi Lin , Quan Yu , Junzheng Peng , Jingang Zhong

This paper reported a general noninterferometric high-accuracy quantitative phase imaging (QPI) method for arbitrary complex valued objects. Given by a typical 4f optical configuration as the imaging system, three frames of small-window…

Image and Video Processing · Electrical Eng. & Systems 2021-05-05 Jianhui Huang , An Pan , Huiliang Jin , Guoxiang Meng , Qian Ye

Hyperspectral 3D imaging aims to acquire both depth and spectral information of a scene. However, existing methods are either prohibitively expensive and bulky or compromise on spectral and depth accuracy. In this work, we present Dispersed…

Image and Video Processing · Electrical Eng. & Systems 2024-03-26 Suhyun Shin , Seokjun Choi , Felix Heide , Seung-Hwan Baek

The structural complexity and instability of many interference phase microscopy methods are the major obstacles toward high-precision phase measurement. In this vein, improving more efficient configurations as well as proposing new methods…

Optics · Physics 2020-01-08 Samira Ebrahimi , Masoomeh Dashtdar

We present a tomographic imaging technique, termed Deep Prior Diffraction Tomography (DP-DT), to reconstruct the 3D refractive index (RI) of thick biological samples at high resolution from a sequence of low-resolution images collected…

Image and Video Processing · Electrical Eng. & Systems 2021-11-18 Kevin C. Zhou , Roarke Horstmeyer

Super-resolution optical microscopy is a rapidly evolving scientific field dedicated to imaging sub-wavelength sized objects, leaving its mark in multiple branches of biology and technology. While several super-resolution optical microscopy…

In Fourier ptychography, multiple low resolution images are captured and subsequently combined computationally into a high-resolution, large-field of view micrograph. A theoretical image-formation model based on the assumption of plane-wave…

Optics · Physics 2022-06-22 Tomas Aidukas , Lars Loetgering , Andrew Robert Harvey

Modern Flash X-ray diffraction Imaging (FXI) acquires diffraction signals from single biomolecules at a high repetition rate from X-ray Free Electron Lasers (XFELs), easily obtaining millions of 2D diffraction patterns from a single…

Image and Video Processing · Electrical Eng. & Systems 2020-10-28 Jing Liu , Stefan Engblom , Carl Nettelblad

Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the universal…

High-resolution diffusion tensor imaging (DTI) is beneficial for probing tissue microstructure in fine neuroanatomical structures, but long scan times and limited signal-to-noise ratio pose significant barriers to acquiring DTI at…

Image and Video Processing · Electrical Eng. & Systems 2021-02-19 Qiyuan Tian , Ziyu Li , Qiuyun Fan , Chanon Ngamsombat , Yuxin Hu , Congyu Liao , Fuyixue Wang , Kawin Setsompop , Jonathan R. Polimeni , Berkin Bilgic , Susie Y. Huang

Confocal fluorescence microscopy is one of the most accessible and widely used imaging techniques for the study of biological processes at the cellular and subcellular levels. Scanning confocal microscopy allows the capture of high-quality…

Image and Video Processing · Electrical Eng. & Systems 2024-11-01 Soufiane Belharbi , Mara KM Whitford , Phuong Hoang , Shakeeb Murtaza , Luke McCaffrey , Eric Granger

We demonstrate a new computational illumination technique that achieves large space-bandwidth-time product, for quantitative phase imaging of unstained live samples in vitro. Microscope lenses can have either large field of view (FOV) or…

Optics · Physics 2015-10-16 Lei Tian , Ziji Liu , Li-Hao Yeh , Michael Chen , Jingshan Zhong , Laura Waller

The photo-kinetics of fluorescent molecules have enabled the circumvention of far-field optical diffraction-limit. Despite its enormous potential, the necessity to label the sample may adversely influence the delicate biology under…

The principles of quantum optics have yielded a plethora of ideas to surpass the classical limitations of sensitivity and resolution in optical microscopy. While some ideas have been applied in proof-of-principle experiments, imaging a…

Quantitative phase imaging (QPI) enables visualization and quantitative extraction of the optical phase information of transparent samples. However, conventional QPI techniques typically rely on multi-frame acquisition or complex…

Selective plane illumination microscopy (SPIM), also known as light sheet fluorescence microscopy, provides high specificity through fluorescence labeling. However, it lacks complementary structural information from the surrounding context,…

Optics · Physics 2024-12-19 Rui Ma , Olga Lyraki , Daniel Wehner , Jochen Guck

Hyperspectral imaging (HSI) captures spatial and spectral data, enabling analysis of features invisible to conventional systems. The technology is vital in fields such as weather monitoring, food quality control, counterfeit detection,…

Computer Vision and Pattern Recognition · Computer Science 2025-02-12 David S. Bhatti , Yougin Choi , Rahman S M Wahidur , Maleeka Bakhtawar , Sumin Kim , Surin Lee , Yongtae Lee , Heung-No Lee

In this work, we present a highly efficient quantitative phase imaging (QPI) approach using programmable annular LED illumination based on traditional bright-field microscope. As a new type of coded illumination, the LED array provides a…

Optics · Physics 2017-07-14 Jiaji Li , Qian Chen , Jialin Zhang , Yan Zhang , Linpeng Lu , Chao Zuo

Purpose: Diffusion MRI (dMRI) provides a diverse set of quantitative measures and derived datatypes to assess white matter microstructure and macrostructure. Coupled with the increasing size of imaging studies using dMRI, the number of…

This paper presents an innovative set of tools to support a methodology for the multichannel interpolation (MCI) of a discrete signal. It is shown that a bandlimited signal $f$ can be exactly reconstructed from finite samples of $g_k$…

Information Theory · Computer Science 2019-04-12 Dong Cheng , Kit Ian Kou