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Ensemble averaging experiments may conceal many fundamental molecular interactions. To overcome that, a high-throughput detection of single molecules or colloidal nanodots is crucial for biomedical, nanoelectronic, and solid-state…

Biological Physics · Physics 2019-07-15 Siddharth Ghosh , Narain Karedla , Ingo Gregor

Optical-based nanothermometry represents a transformative approach for precise temperature measurements at the nanoscale, which finds versatile applications across biology, medicine, and electronics. The assembly of ratiometric fluorescent…

Optics · Physics 2025-04-01 Mahshid Iraniparast , Nishant Kumar , Igor Sokolov

Particle size is a key variable in understanding the behaviour of the particulate products that underpin much of our modern lives. Typically obtained from suspensions at rest, measuring the particle size under flowing conditions would…

Soft Condensed Matter · Physics 2021-07-22 James A Richards , Vincent A Martinez , Jochen Arlt

We introduce an image cytometer (I-CYT) for the analysis of phytoplankton in fresh and marine water environments. A linear quantification of cell numbers was observed covering several orders of magnitude using cultures of Tetraselmis and…

Quantitative Methods · Quantitative Biology 2016-11-23 J. M. Pérez , M. Jofre , P. Martínez , M. A. Yáñez , V. Catalan , A. Parker , M. Veldhuis , V. Pruneri

Semiconductor nanoparticles (quantum dots) were studied in the context of liquid scintillator development for upcoming neutrino experiments. The unique optical and chemical properties of quantum dots are particularly promising for the use…

Instrumentation and Detectors · Physics 2015-06-16 C. Aberle , J. J. Li , S. Weiss , L. Winslow

The investigation of liquid flow at the nanoscale is a key area of applied research with high relevance to Physics, Chemistry and Biology. We introduce a method and a device that allows to spatially resolve liquid flow by integrating an…

Mesoscale and Nanoscale Physics · Physics 2016-08-24 R. F. Neumann , M. Engel , M. Steiner

Significance: Measuring cerebral blood flow (CBF) is crucial for diagnosing various cerebral diseases. An affordable, wearable, and fiber-free continuous-wave speckle contrast flowmetry (CW-DSCF) technique has been developed for continuous…

Quantitative Methods · Quantitative Biology 2025-02-13 Chaebeom Yeo , Xuhui Liu , Mehrana Mohtasebi , Faezeh Akbari , Faraneh Fathi , Guoqiang Yu

We introduce a new image cytometer design for detection of very small particulate and demonstrate its capability in water analysis. The device is a compact microscope composed of off the shelf components, such as a light emitting diode…

Optics · Physics 2016-02-17 Juan Miguel Pérez , Marc Jofre , Pedro Martínez , Valerio Pruneri

An efficient numerical method based on half-space Green function and spherical harmonics expansion is used to study the light scattering from coupled multiple nanospheres on a substrate. The ellipsometric spectra for various geometries of…

Mesoscale and Nanoscale Physics · Physics 2015-06-16 Huai-Yi Xie , Yia-Chung Chang

Extracellular vesicles (EVs) and plasma membrane-derived exosome-mimetic nanovesicles demonstrate significant potential for drug delivery. Latter synthetic provides higher throughput over physiological EVs. However they face size-stability…

Biological Physics · Physics 2023-12-07 Maryam Sanaee , K. Göran Ronquist , Jane M. Morrell , Muhammet S. Toprak , Katia Gallo

By using scattering in near field techniques, a microscope can be easily turned into a device measuring static and dynamic light scattering, very useful for the characterization of nanoparticle dispersions. Up to now, microscopy based…

Optics · Physics 2015-05-13 D. Brogioli , D. Salerno , V. Cassina , F. Mantegazza

We present a feature-resolved methodology to analyse the photoluminescence dynamics of single emitters using a combination of lifetime, spectral, and photon correlation analyses. By integrating conventional ensemble photon statistics…

Biological nanoparticles (BNPs) are of high interest due to their key role in various biological processes and use as biomarkers. BNP size and molecular composition are decisive for their functions, but simultaneous determination of both…

Biological Physics · Physics 2016-09-26 Stephan Block , Björn Johansson Fast , Anders Lundgren , Vladimir P. Zhdanov , Fredrik Höök

This study introduces chromatic calorimetry, a novel particle detection method that uses strategically layered scintillators with different emission wavelengths. This approach aims to enhance energy measurement by capturing particle…

Instrumentation and Detectors · Physics 2025-01-16 Devanshi Arora , Matteo Salomoni , Yacine Haddad , Vojtech Zabloudil , Michael Doser , Masaki Owari , Etiennette Auffray

Microscopic dynamics reveal the origin of the bulk rheological response in complex fluids. In model systems particle motion can be tracked, but for industrially relevant samples this is often impossible. Here we adapt differential dynamic…

Soft Condensed Matter · Physics 2022-02-23 James A. Richards , Vincent A. Martinez , Jochen Arlt

Single-molecule surface enhanced Raman scattering (SM-SERS) is one of the vital applications of plasmonic nanoparticles. The SM-SERS sensitivity critically depends on plasmonic hot-spots created at the vicinity of such nanoparticles. In…

We report on a new method to characterize nano-hydrodynamic properties at the liquid/solid interface relying solely on the measurement of the thermal motion of confined colloids. Using Fluorescence Correlation Spectroscopy (FCS) to probe…

Soft Condensed Matter · Physics 2015-06-25 L. Joly , C. Ybert , L. Bocquet

The use of a scanning flow cytometer (SFC) to study the evolution of monomers, dimers and higher multimers of latex particles at the initial stage of the immunoagglutination is described. The SFC can measure the light-scattering pattern…

Aerosol injectors applied in single-particle diffractive imaging experiments demonstrated their potential in efficiently delivering nanoparticles with high density. Continuous optimization of injector design is crucial for achieving…

Droplet microfluidics offers a versatile platform for analyzing liquid samples. Despite its potential, there is a lack of techniques that allow to reliably probe individual circulating droplets. The prospect of combining droplet…

Quantitative Methods · Quantitative Biology 2024-03-08 Marc Sulliger , Jaime Ortega Arroyo , Romain Quidant