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Cryo-electron microscopy provides 2-D projection images of the 3-D electron scattering intensity of many instances of the particle under study (e.g., a virus). Both symmetry (rotational point groups) and heterogeneity are important aspects…

Applications · Statistics 2019-05-17 Nan Xu , Peter C Doerschuk

Single particle cryo-electron microscopy (EM) is an increasingly popular method for determining the 3-D structure of macromolecules from noisy 2-D images of single macromolecules whose orientations and positions are random and unknown. One…

Computer Vision and Pattern Recognition · Computer Science 2017-04-11 Roy R. Lederman , Amit Singer

Single-particle electron cryomicroscopy is an essential tool for high-resolution 3D reconstruction of proteins and other biological macromolecules. An important challenge in cryo-EM is the reconstruction of non-rigid molecules with parts…

Image and Video Processing · Electrical Eng. & Systems 2021-05-31 Amit Moscovich , Amit Halevi , Joakim Andén , Amit Singer

One of the difficulties in 3D reconstruction of molecules from images in single particle Cryo-Electron Microscopy (Cryo-EM), in addition to high levels of noise and unknown image orientations, is heterogeneity in samples: in many cases, the…

Computer Vision and Pattern Recognition · Computer Science 2016-07-13 Roy R. Lederman , Amit Singer

Cryo-electron microscopy (Cryo-EM) enables high-resolution imaging of biomolecules, but structural heterogeneity remains a major challenge in 3D reconstruction. Traditional methods assume a discrete set of conformations, limiting their…

Machine Learning · Statistics 2025-09-09 Diego Sanchez Espinosa , Erik H Thiede , Yunan Yang

Cryogenic electron microscopy (cryo-EM) is an invaluable technique for determining high-resolution three-dimensional structures of biological macromolecules using transmission particle images. The inherent symmetry in these macromolecules…

Optimization and Control · Mathematics 2024-11-06 Qi Zhang , Chenglong Bao , Hai Lin , Mingxu Hu

In this paper, we address the problem of 3D object mesh reconstruction from RGB videos. Our approach combines the best of multi-view geometric and data-driven methods for 3D reconstruction by optimizing object meshes for multi-view…

Computer Vision and Pattern Recognition · Computer Science 2019-03-21 Chen-Hsuan Lin , Oliver Wang , Bryan C. Russell , Eli Shechtman , Vladimir G. Kim , Matthew Fisher , Simon Lucey

Recovering the 3D structure of an object from a single image is a challenging task due to its ill-posed nature. One approach is to utilize the plentiful photos of the same object category to learn a strong 3D shape prior for the object.…

Computer Vision and Pattern Recognition · Computer Science 2021-09-08 Long-Nhat Ho , Anh Tuan Tran , Quynh Phung , Minh Hoai

Recovering the 3D geometry of a purely texture-less object with generally unknown surface reflectance (e.g. non-Lambertian) is regarded as a challenging task in multi-view reconstruction. The major obstacle revolves around establishing…

Computer Vision and Pattern Recognition · Computer Science 2021-05-26 Ziang Cheng , Hongdong Li , Yuta Asano , Yinqiang Zheng , Imari Sato

Recent advances have enabled 3d object reconstruction approaches using a single off-the-shelf RGB-D camera. Although these approaches are successful for a wide range of object classes, they rely on stable and distinctive geometric or…

Computer Vision and Pattern Recognition · Computer Science 2017-04-04 Dimitrios Tzionas , Juergen Gall

We investigate the recovery of structures from large-area, low dose exposures that distribute the dose over many identical copies of an object. The reconstruction is done via a maximum likelihood approach that does neither require to…

Materials Science · Physics 2018-03-28 C. Kramberger , J. C. Meyer

The graph projection of a hypergraph is a simple graph with the same vertex set and with an edge between each pair of vertices that appear in a hyperedge. We consider the problem of reconstructing a random $d$-uniform hypergraph from its…

Statistics Theory · Mathematics 2025-02-14 Guy Bresler , Chenghao Guo , Yury Polyanskiy

Cryogenic electron microscopy (cryo-EM) has transformed structural biology by allowing to reconstruct 3D biomolecular structures up to near-atomic resolution. However, the 3D reconstruction process remains challenging, as the 3D structures…

Computer Vision and Pattern Recognition · Computer Science 2023-07-18 Bongjin Koo , Julien Martel , Ariana Peck , Axel Levy , Frédéric Poitevin , Nina Miolane

In this paper, we propose a novel method for joint recovery of camera pose, object geometry and spatially-varying Bidirectional Reflectance Distribution Function (svBRDF) of 3D scenes that exceed object-scale and hence cannot be captured…

Computer Vision and Pattern Recognition · Computer Science 2023-06-07 Carolin Schmitt , Božidar Antić , Andrei Neculai , Joo Ho Lee , Andreas Geiger

We introduce the EMC algorithm for reconstructing a particle's 3D diffraction intensity from very many photon shot-noise limited 2D measurements, when the particle orientation in each measurement is unknown. The algorithm combines a…

Data Analysis, Statistics and Probability · Physics 2010-03-04 Duane Ne-Te Loh , Veit Elser

In this paper, we propose a computational framework for 3D volume reconstruction from 2D histological slices using registration algorithms in feature space. To improve the quality of reconstructed 3D volume, first, intensity variations in…

Computer Vision and Pattern Recognition · Computer Science 2009-07-21 Ulas Bagci , Li Bai

Discovering the 3D atomic structure of molecules such as proteins and viruses is a fundamental research problem in biology and medicine. Electron Cryomicroscopy (Cryo-EM) is a promising vision-based technique for structure estimation which…

Computer Vision and Pattern Recognition · Computer Science 2016-11-18 Marcus A. Brubaker , Ali Punjani , David J. Fleet

A modulated icosahedral shell with an inclusion is a concise description of many viruses, including recently-discovered large double-stranded DNA ones. Many X-ray scattering patterns of such viruses show major polygonal fringes, which can…

Biological Physics · Physics 2014-08-08 Aliakbar Jafarpour

Cryo-electron microscopy (cryo-EM) is an experimental technique for protein structure determination that images an ensemble of macromolecules in near-physiological contexts. While recent advances enable the reconstruction of dynamic…

Electron tomography is becoming an increasingly important tool in materials science for studying the three-dimensional morphologies and chemical compositions of nanostructures. The image quality obtained by many current algorithms is…

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