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Confining light illumination in the three dimensions of space is a challenge for various applications. Among these, optogenetic methods developed for live experiments in cell biology would benefit from such a localized illumination as it…

Super-resolution imaging methods that combine interferometric (z) analysis with single-molecule localization microscopy (iSMLM) have achieved ultra-high 3D precision and contributed to the elucidation of important biological…

Optics · Physics 2025-02-20 Wei Wang , Zengxin Huang , Yilin Wang , Hangfeng Li , Pakorn Kanchanawong

Silicon sensors are the most diffuse position sensitive device in particle physics 8 experiments and in countless applications in science and technology. They had a spectacular progress in performance over almost 40 years since their first…

Instrumentation and Detectors · Physics 2022-05-04 Gianluigi Casse , Nicola Massari , Matthew Franksa , Luca Parmesana

The use of photo-activated fluorescent molecules to create long sequences of low emitter-density diffraction-limited images enables high-precision emitter localization, but at the cost of low temporal resolution. We suggest combining…

Image and Video Processing · Electrical Eng. & Systems 2023-07-19 Gili Dardikman-Yoffe , Yonina C. Eldar

We realize a scanning probe microscope using single trapped $^{87}$Rb atoms to measure optical fields with subwavelength spatial resolution. Our microscope operates by detecting fluorescence from a single atom driven by near-resonant light…

Atomic Physics · Physics 2022-03-02 Emma Deist , Justin A. Gerber , Yue-Hui Lu , Johannes Zeiher , Dan M. Stamper-Kurn

Super-resolution imaging using sub-diffraction field localization by micron sized transparent beads (microspheres) was recently demonstrated [1]. Practical applications in microscopy require control over the positioning of the microspheres.…

Optics · Physics 2013-12-25 Leonid A. Krivitsky , Jia Jun Wang , Zengbo Wang , Boris Lukiyanchuk

The study of optical phenomena on the subwavelength scale is becoming increasingly important in photonics, particularly in the fields of nanoemitters, photonic crystals and plasmonics. Subwavelength field patterns are evanescent and must…

Photographic emulsion is a particle tracking device which features the best spatial resolution among particle detectors. For certain applications, for example muon radiography, large-scale detectors are required. Therefore, a huge surface…

Instrumentation and Detectors · Physics 2016-03-07 T. Ariga , A. Ariga , K. Kuwabara , K. Morishima , M. Moto , A. Nishio , P. Scampoli , M. Vladymyrov

Low-light optical imaging refers to the use of cameras to capture images with minimal photon flux. This area has broad application to diverse fields, including optical microscopy for biological studies. In such studies, it is important to…

Recently, it was discovered that microsphere can generate super-resolution focusing beyond diffraction limit. This has led to the development of an exciting super-resolution imaging technique -microsphere nanoscopy- that features a record…

Optics · Physics 2021-08-26 Zengbo Wang

The size- and fluorescence-based sorting of micro- and nano-scale particles suspended in fluid presents a significant and important challenge for both sample analysis and for manufacturing of nanoparticle-based products. Here we demonstrate…

Soft Condensed Matter · Physics 2015-02-16 Sukumar Rajauria , Christopher Axline , Claudia Gottstein , Andrew N. Cleland

We propose a novel, to the best of our knowledge, approach to superresolution optical imaging by combining quantum optics and near-field optics. Our concept involves the utilization of single-photon quantum emitters to generate a…

Quantum Physics · Physics 2024-03-26 Aziz Kolkiran

Light microscopy as well as image acquisition and processing suffer from physical and technical prejudices which preclude a correct interpretation of biological observations which can be reflected in, e.g., medical and pharmacological…

Quantitative Methods · Quantitative Biology 2019-08-15 Renata Rychtarikova , Georg Steiner , Gero Kramer , Michael B. Fischer , Dalibor Stys

Super-resolution microscopy is rapidly gaining importance as an analytical tool in the life sciences. A compelling feature is the ability to label biological units of interest with fluorescent markers in living cells and to observe them…

Controlling quantum light-matter interactions at scales smaller than the diffraction limit at the single quantum emitter level is a critical challenge to the goal of advancing quantum technologies. We introduce a novel material platform…

We propose a method of spatial resolution enhancement in metrology (thermometry, magnetometry, pH estimation, and similar methods) with blinking fluorescent nanosensors by combining sensing with super-resolution optical fluctuation imaging…

Optics · Physics 2026-05-11 Alexander Mikhalychev , Alex Ulyanenkov

Molecular interactions are key to many chemical and biological processes like protein function. In many signaling processes they occur in sub-cellular areas displaying nanoscale organizations and involving molecular assemblies. The…

Here, we report analysis and summary of research in the field of localization microscopy for optical imaging. We introduce the basic elements of super-resolved localization microscopy methods for PALM and STORM, commonly used both in vivo…

Optics · Physics 2021-02-25 Jack W Shepherd , Mark C Leake

Traceability to the International System of Units (SI) is fundamental to measurement accuracy and reliability. In this study, we demonstrate subnanometer traceability of localization microscopy, establishing a metrological foundation for…

Event-based image sensors provide microsecond temporal resolution but lack spectral discrimination, whereas diffractive spectral imagers encode wavelength information at conventional frame rates. We introduce a fluorescence microscopy…

Optics · Physics 2026-03-20 Richard G. Baird , Boyden Myers , Erik M. Jorgensen , Rajesh Menon